Protein deterioration and longevity of quinoa seeds during long-term storage

Seed protein stability and seed deterioration during storage were studied in seeds of two different quinoa (Chenopodium quinoa Willd.) cultivars, i.e., cv. Ollagüe and cv. Baer II. Germination and viability tests proved that cv. Baer II was more longevous than cv. Ollagüe. Protein insolubilisation w...

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Autores principales: Castellión, M., Matiacevich, S., Buera, P., Maldonado, S.
Formato: JOUR
Materias:
Chenopodium quinoa
Maillard
Protein solubility
Seed ageing
Seed conservation
Seed storage proteins
advanced glycation end product
article
conservational seed bank
controlled study
deterioration
germination
glycation
nonhuman
plant seed
protein solubility
protein stability
protein structure, function and variability
seed storage
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03088146_v121_n4_p952_Castellion
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03088146_v121_n4_p952_Castellion
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spelling todo:paper_03088146_v121_n4_p952_Castellion2023-10-03T15:22:52Z Protein deterioration and longevity of quinoa seeds during long-term storage Castellión, M. Matiacevich, S. Buera, P. Maldonado, S. Chenopodium quinoa Maillard Protein solubility Seed ageing Seed conservation Seed storage proteins advanced glycation end product article Chenopodium quinoa conservational seed bank controlled study deterioration germination glycation nonhuman plant seed protein solubility protein stability protein structure, function and variability seed storage Chenopodium quinoa Seed protein stability and seed deterioration during storage were studied in seeds of two different quinoa (Chenopodium quinoa Willd.) cultivars, i.e., cv. Ollagüe and cv. Baer II. Germination and viability tests proved that cv. Baer II was more longevous than cv. Ollagüe. Protein insolubilisation was detected during storage and correlated with longevity. However, protein solubility was restored by priming in both cultivars, disregarding their germination capability. Extremely high contents of advanced glycation end-products (AGEs), assessed by carboxymethyllysine (CML) quantification, were obtained from stored seeds as viability was reduced; primed seeds showed slightly lower AGE content, indicating a strong association between quinoa seed ageing and Maillard products accumulation. High intensity fluorescence values were observed in seeds stored in the gene bank bearing 100% germination values, which indicate that the detection of fluorescence is not by itself an appropriate indicator of protein damage by Maillard-type product accumulation in quinoa seeds. This work establishes for the first time a direct association between seed viability and AGE accumulation, due to Maillard reaction, in quinoa seeds. © 2010 Elsevier Ltd. All rights reserved. Fil:Castellión, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matiacevich, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Buera, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03088146_v121_n4_p952_Castellion
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Chenopodium quinoa
Maillard
Protein solubility
Seed ageing
Seed conservation
Seed storage proteins
advanced glycation end product
article
Chenopodium quinoa
conservational seed bank
controlled study
deterioration
germination
glycation
nonhuman
plant seed
protein solubility
protein stability
protein structure, function and variability
seed storage
Chenopodium quinoa
spellingShingle Chenopodium quinoa
Maillard
Protein solubility
Seed ageing
Seed conservation
Seed storage proteins
advanced glycation end product
article
Chenopodium quinoa
conservational seed bank
controlled study
deterioration
germination
glycation
nonhuman
plant seed
protein solubility
protein stability
protein structure, function and variability
seed storage
Chenopodium quinoa
Castellión, M.
Matiacevich, S.
Buera, P.
Maldonado, S.
Protein deterioration and longevity of quinoa seeds during long-term storage
topic_facet Chenopodium quinoa
Maillard
Protein solubility
Seed ageing
Seed conservation
Seed storage proteins
advanced glycation end product
article
Chenopodium quinoa
conservational seed bank
controlled study
deterioration
germination
glycation
nonhuman
plant seed
protein solubility
protein stability
protein structure, function and variability
seed storage
Chenopodium quinoa
description Seed protein stability and seed deterioration during storage were studied in seeds of two different quinoa (Chenopodium quinoa Willd.) cultivars, i.e., cv. Ollagüe and cv. Baer II. Germination and viability tests proved that cv. Baer II was more longevous than cv. Ollagüe. Protein insolubilisation was detected during storage and correlated with longevity. However, protein solubility was restored by priming in both cultivars, disregarding their germination capability. Extremely high contents of advanced glycation end-products (AGEs), assessed by carboxymethyllysine (CML) quantification, were obtained from stored seeds as viability was reduced; primed seeds showed slightly lower AGE content, indicating a strong association between quinoa seed ageing and Maillard products accumulation. High intensity fluorescence values were observed in seeds stored in the gene bank bearing 100% germination values, which indicate that the detection of fluorescence is not by itself an appropriate indicator of protein damage by Maillard-type product accumulation in quinoa seeds. This work establishes for the first time a direct association between seed viability and AGE accumulation, due to Maillard reaction, in quinoa seeds. © 2010 Elsevier Ltd. All rights reserved.
format JOUR
author Castellión, M.
Matiacevich, S.
Buera, P.
Maldonado, S.
author_facet Castellión, M.
Matiacevich, S.
Buera, P.
Maldonado, S.
author_sort Castellión, M.
title Protein deterioration and longevity of quinoa seeds during long-term storage
title_short Protein deterioration and longevity of quinoa seeds during long-term storage
title_full Protein deterioration and longevity of quinoa seeds during long-term storage
title_fullStr Protein deterioration and longevity of quinoa seeds during long-term storage
title_full_unstemmed Protein deterioration and longevity of quinoa seeds during long-term storage
title_sort protein deterioration and longevity of quinoa seeds during long-term storage
url http://hdl.handle.net/20.500.12110/paper_03088146_v121_n4_p952_Castellion
work_keys_str_mv AT castellionm proteindeteriorationandlongevityofquinoaseedsduringlongtermstorage
AT matiacevichs proteindeteriorationandlongevityofquinoaseedsduringlongtermstorage
AT buerap proteindeteriorationandlongevityofquinoaseedsduringlongtermstorage
AT maldonados proteindeteriorationandlongevityofquinoaseedsduringlongtermstorage
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