Patterns of variation in desiccation resistance in a set of recombinant inbred lines in Drosophila melanogaster

Desiccation, resulting from extremely dry environmental conditions, is a serious obstacle to the survival of organisms. Water is vital for the maintenance of intracellular structure and prevents the irreversible formation of aggregates, an occurrence leading to loss of cellular function. To characte...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Gomez, F.H., Sambucetti, P.D., Loeschcke, V., Norry, F.M.
Formato: JOUR
Materias:
Dehydration stress
Environmental stress
Small insects
Thermal stress
dehydration
desiccation
environmental conditions
environmental stress
fly
genetic structure
genetic variation
humidity
inbreeding
recombination
survival
temperature effect
Drosophila melanogaster
Hexapoda
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03076962_v40_n3_p205_Gomez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03076962_v40_n3_p205_Gomez
record_format dspace
spelling todo:paper_03076962_v40_n3_p205_Gomez2023-10-03T15:22:43Z Patterns of variation in desiccation resistance in a set of recombinant inbred lines in Drosophila melanogaster Gomez, F.H. Sambucetti, P.D. Loeschcke, V. Norry, F.M. Dehydration stress Environmental stress Small insects Thermal stress dehydration desiccation environmental conditions environmental stress fly genetic structure genetic variation humidity inbreeding recombination survival temperature effect Drosophila melanogaster Hexapoda Desiccation, resulting from extremely dry environmental conditions, is a serious obstacle to the survival of organisms. Water is vital for the maintenance of intracellular structure and prevents the irreversible formation of aggregates, an occurrence leading to loss of cellular function. To characterize genetic variation in desiccation stress resistance (DSR) in Drosophila melanogaster Meigen, an intercontinental set of recombinant inbred lines (RIL) is used. Flies are exposed to a low humidity environment (<10% relative humidity) at a constant temperature of 25°C. Desiccation stress resistance is higher in RIL derived from a backcross to the parental stock sensitive to heat stress (from Denmark) than in RIL derived from the reciprocal backcross to the heat-stress resistant stock (from Australia). Composite interval mapping reveals significant quantitative trail loci (QTL) for DSR in the set of RIL. Both major and minor effects QTL are detected, suggesting a complex genetic architecture. When compared with a previous investigation performed on the same set of RIL, the present study indicates that not all traits of resistance to environmental stressors are affected in the same direction by segregating co-localized QTL. © 2015 The Royal Entomological Society. Fil:Gomez, F.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sambucetti, P.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Norry, F.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03076962_v40_n3_p205_Gomez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Dehydration stress
Environmental stress
Small insects
Thermal stress
dehydration
desiccation
environmental conditions
environmental stress
fly
genetic structure
genetic variation
humidity
inbreeding
recombination
survival
temperature effect
Drosophila melanogaster
Hexapoda
spellingShingle Dehydration stress
Environmental stress
Small insects
Thermal stress
dehydration
desiccation
environmental conditions
environmental stress
fly
genetic structure
genetic variation
humidity
inbreeding
recombination
survival
temperature effect
Drosophila melanogaster
Hexapoda
Gomez, F.H.
Sambucetti, P.D.
Loeschcke, V.
Norry, F.M.
Patterns of variation in desiccation resistance in a set of recombinant inbred lines in Drosophila melanogaster
topic_facet Dehydration stress
Environmental stress
Small insects
Thermal stress
dehydration
desiccation
environmental conditions
environmental stress
fly
genetic structure
genetic variation
humidity
inbreeding
recombination
survival
temperature effect
Drosophila melanogaster
Hexapoda
description Desiccation, resulting from extremely dry environmental conditions, is a serious obstacle to the survival of organisms. Water is vital for the maintenance of intracellular structure and prevents the irreversible formation of aggregates, an occurrence leading to loss of cellular function. To characterize genetic variation in desiccation stress resistance (DSR) in Drosophila melanogaster Meigen, an intercontinental set of recombinant inbred lines (RIL) is used. Flies are exposed to a low humidity environment (<10% relative humidity) at a constant temperature of 25°C. Desiccation stress resistance is higher in RIL derived from a backcross to the parental stock sensitive to heat stress (from Denmark) than in RIL derived from the reciprocal backcross to the heat-stress resistant stock (from Australia). Composite interval mapping reveals significant quantitative trail loci (QTL) for DSR in the set of RIL. Both major and minor effects QTL are detected, suggesting a complex genetic architecture. When compared with a previous investigation performed on the same set of RIL, the present study indicates that not all traits of resistance to environmental stressors are affected in the same direction by segregating co-localized QTL. © 2015 The Royal Entomological Society.
format JOUR
author Gomez, F.H.
Sambucetti, P.D.
Loeschcke, V.
Norry, F.M.
author_facet Gomez, F.H.
Sambucetti, P.D.
Loeschcke, V.
Norry, F.M.
author_sort Gomez, F.H.
title Patterns of variation in desiccation resistance in a set of recombinant inbred lines in Drosophila melanogaster
title_short Patterns of variation in desiccation resistance in a set of recombinant inbred lines in Drosophila melanogaster
title_full Patterns of variation in desiccation resistance in a set of recombinant inbred lines in Drosophila melanogaster
title_fullStr Patterns of variation in desiccation resistance in a set of recombinant inbred lines in Drosophila melanogaster
title_full_unstemmed Patterns of variation in desiccation resistance in a set of recombinant inbred lines in Drosophila melanogaster
title_sort patterns of variation in desiccation resistance in a set of recombinant inbred lines in drosophila melanogaster
url http://hdl.handle.net/20.500.12110/paper_03076962_v40_n3_p205_Gomez
work_keys_str_mv AT gomezfh patternsofvariationindesiccationresistanceinasetofrecombinantinbredlinesindrosophilamelanogaster
AT sambucettipd patternsofvariationindesiccationresistanceinasetofrecombinantinbredlinesindrosophilamelanogaster
AT loeschckev patternsofvariationindesiccationresistanceinasetofrecombinantinbredlinesindrosophilamelanogaster
AT norryfm patternsofvariationindesiccationresistanceinasetofrecombinantinbredlinesindrosophilamelanogaster
_version_ 1782026306640150528

Ejemplares similares