N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants

Drosophila melanogaster Meigen mutants for N-β-alanyldopamine (NBAD) metabolism have altered levels of NBAD, dopamine and other neurotransmitters. The ebony1 mutant strain has very low levels of NBAD and higher levels of dopamine, whereas the opposite situation is observed in the tan1 mutant. Dopami...

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Autores principales: Rossi, F.A., Bochicchio, P.A., Quesada-Allué, L.A., Pérez, M.M.
Formato: JOUR
Materias:
Dopamine
ebony
Locomotor activity
N-β-alanyldopamine
Sleep
tan
Wild-type
enzyme activity
fly
locomotion
metabolism
mutation
sleep
Drosophila melanogaster
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03076962_v40_n2_p166_Rossi
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_03076962_v40_n2_p166_Rossi2023-10-03T15:22:43Z N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants Rossi, F.A. Bochicchio, P.A. Quesada-Allué, L.A. Pérez, M.M. Dopamine ebony Locomotor activity N-β-alanyldopamine Sleep tan Wild-type enzyme activity fly locomotion metabolism mutation sleep Drosophila melanogaster Drosophila melanogaster Meigen mutants for N-β-alanyldopamine (NBAD) metabolism have altered levels of NBAD, dopamine and other neurotransmitters. The ebony1 mutant strain has very low levels of NBAD and higher levels of dopamine, whereas the opposite situation is observed in the tan1 mutant. Dopamine is implicated in the control of movement, memory and arousal, as well as in the regulation of sleep and wakefulness in D. melanogaster. N-β-alanyldopamine, which is best known as a cuticle cross-linking agent, is also present in nervous tissue and has been proposed to promote locomotor activity in this fly. The daily locomotor activity and the sleep patterns of ebony1 and tan1 mutants are analyzed, and are compared with wild-type flies. The tan1 mutant shows reduced locomotor activity, whereas ebony1 shows higher levels of activity than wild-type flies, suggesting that NBAD does not promote locomotor activity. Both mutants spend less time asleep than wild-type flies during night-time; ebony shows more consolidated activity during night-time and increased sleep latency, whereas tan is unable to consolidate locomotor activity and sleep in either phase of the day. The daily level of NBAD-synthase activity is measured in vitro using wild-type and tan1 protein extracts, and the lowest NBAD synthesis is observed at the time of higher locomotor activity. The abnormalities in several parameters of the waking/sleep cycle indicate some dysfunction in the processes that regulates these behaviours in both mutants. © 2015 The Royal Entomological Society. Fil:Quesada-Allué, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérez, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03076962_v40_n2_p166_Rossi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Dopamine
ebony
Locomotor activity
N-β-alanyldopamine
Sleep
tan
Wild-type
enzyme activity
fly
locomotion
metabolism
mutation
sleep
Drosophila melanogaster
spellingShingle Dopamine
ebony
Locomotor activity
N-β-alanyldopamine
Sleep
tan
Wild-type
enzyme activity
fly
locomotion
metabolism
mutation
sleep
Drosophila melanogaster
Rossi, F.A.
Bochicchio, P.A.
Quesada-Allué, L.A.
Pérez, M.M.
N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
topic_facet Dopamine
ebony
Locomotor activity
N-β-alanyldopamine
Sleep
tan
Wild-type
enzyme activity
fly
locomotion
metabolism
mutation
sleep
Drosophila melanogaster
description Drosophila melanogaster Meigen mutants for N-β-alanyldopamine (NBAD) metabolism have altered levels of NBAD, dopamine and other neurotransmitters. The ebony1 mutant strain has very low levels of NBAD and higher levels of dopamine, whereas the opposite situation is observed in the tan1 mutant. Dopamine is implicated in the control of movement, memory and arousal, as well as in the regulation of sleep and wakefulness in D. melanogaster. N-β-alanyldopamine, which is best known as a cuticle cross-linking agent, is also present in nervous tissue and has been proposed to promote locomotor activity in this fly. The daily locomotor activity and the sleep patterns of ebony1 and tan1 mutants are analyzed, and are compared with wild-type flies. The tan1 mutant shows reduced locomotor activity, whereas ebony1 shows higher levels of activity than wild-type flies, suggesting that NBAD does not promote locomotor activity. Both mutants spend less time asleep than wild-type flies during night-time; ebony shows more consolidated activity during night-time and increased sleep latency, whereas tan is unable to consolidate locomotor activity and sleep in either phase of the day. The daily level of NBAD-synthase activity is measured in vitro using wild-type and tan1 protein extracts, and the lowest NBAD synthesis is observed at the time of higher locomotor activity. The abnormalities in several parameters of the waking/sleep cycle indicate some dysfunction in the processes that regulates these behaviours in both mutants. © 2015 The Royal Entomological Society.
format JOUR
author Rossi, F.A.
Bochicchio, P.A.
Quesada-Allué, L.A.
Pérez, M.M.
author_facet Rossi, F.A.
Bochicchio, P.A.
Quesada-Allué, L.A.
Pérez, M.M.
author_sort Rossi, F.A.
title N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title_short N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title_full N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title_fullStr N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title_full_unstemmed N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title_sort n-β-alanyldopamine metabolism, locomotor activity and sleep in drosophila melanogaster ebony and tan mutants
url http://hdl.handle.net/20.500.12110/paper_03076962_v40_n2_p166_Rossi
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AT bochicchiopa nbalanyldopaminemetabolismlocomotoractivityandsleepindrosophilamelanogasterebonyandtanmutants
AT quesadaalluela nbalanyldopaminemetabolismlocomotoractivityandsleepindrosophilamelanogasterebonyandtanmutants
AT perezmm nbalanyldopaminemetabolismlocomotoractivityandsleepindrosophilamelanogasterebonyandtanmutants
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