Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa)

Background and Aims In mature quinoa (Chenopodium quinoa) seeds, the lasting endosperm forms a micropylar cone covering the radicle. The suspensor cells lie within the centre of the cone. During the final stage of seed development, the cells of the lasting endosperm accumulate protein and lipids whi...

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Autores principales:	López-Fernández, M.P., Maldonado, S.
Formato:	JOUR
Materias:	Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot Cysteine endopeptidase Endosperm Micropylar endosperm PCD Programmed cell death Ricinosomes Suspensor TUNEL. abnormality bioassay biological development DNA enzyme activity germination immune response lipid maturation protein seed sperm cysteine proteinase apoptosis article cell death cell fractionation cell nucleus cell organelle cytology DNA fragmentation endosperm enzymology flow cytometry growth, development and aging metabolism micropylar endosperm nick end labeling ricinosomes suspensor ultrastructure cysteine endopeptidase programmed cell death TUNEL Cell Death Cell Nucleus Cysteine Endopeptidases DNA Fragmentation Flow Cytometry Organelles Subcellular Fractions
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03057364_v112_n7_p1253_LopezFernandez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03057364_v112_n7_p1253_LopezFernandez
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spelling	todo:paper_03057364_v112_n7_p1253_LopezFernandez2023-10-03T15:21:55Z Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa) López-Fernández, M.P. Maldonado, S. Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot Cysteine endopeptidase Endosperm Micropylar endosperm PCD Programmed cell death Ricinosomes Suspensor TUNEL. abnormality bioassay biological development DNA enzyme activity germination immune response lipid maturation protein seed sperm cysteine proteinase Amaranthaceae apoptosis article cell death cell fractionation cell nucleus cell organelle Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cytology DNA fragmentation endosperm enzymology flow cytometry growth, development and aging metabolism micropylar endosperm nick end labeling PCD ricinosomes suspensor ultrastructure Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cysteine endopeptidase endosperm micropylar endosperm PCD programmed cell death ricinosomes suspensor TUNEL Cell Death Cell Nucleus Chenopodium quinoa Cysteine Endopeptidases DNA Fragmentation Endosperm Flow Cytometry Organelles Subcellular Fractions Background and Aims In mature quinoa (Chenopodium quinoa) seeds, the lasting endosperm forms a micropylar cone covering the radicle. The suspensor cells lie within the centre of the cone. During the final stage of seed development, the cells of the lasting endosperm accumulate protein and lipids while the rest are crushed and disintegrated. Both the suspensor and endosperm die progressively from the innermost layers surrounding the embryo and extending towards the nucellar tissue. Ricinosomes are endoplasmic reticulum-derived organelles that accumulate both the pro-form and the mature form of cysteine endopeptidase (Cys-EP), first identified in castor bean (Ricinus communis) endosperm during germination. This study sought to identify associations between the presence of ricinosomes and programmed cell death (PCD) hallmarks in suspensor and endosperm cells predestined to die during quinoa seed development. MethodsAstructural study using light microscopy and transmission electron microscopywas performed. To detect the presence of Cys-EP, both western blot and in situ immunolocalization assays were carried out using anti- R. communis Cys-EP antibody. ATUNEL assay was used to determine DNA fragmentation. Results and Conclusions Except for the one or two cell layers that constitute the lasting endosperm in the mature seed, ricinosomes were found in suspensor and endosperm cells. These cells were also the site of morphological abnormalities, including misshapen and fragmented nuclei, vesiculation of the cytosol, vacuole collapse and cell wall disorganization. It is proposed that, in suspensor and endosperm cells, the early detection of Cys-EP in ricinosomes predicts the occurrence of PCD during late seed development. © The Author 2013. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03057364_v112_n7_p1253_LopezFernandez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot Cysteine endopeptidase Endosperm Micropylar endosperm PCD Programmed cell death Ricinosomes Suspensor TUNEL. abnormality bioassay biological development DNA enzyme activity germination immune response lipid maturation protein seed sperm cysteine proteinase Amaranthaceae apoptosis article cell death cell fractionation cell nucleus cell organelle Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cytology DNA fragmentation endosperm enzymology flow cytometry growth, development and aging metabolism micropylar endosperm nick end labeling PCD ricinosomes suspensor ultrastructure Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cysteine endopeptidase endosperm micropylar endosperm PCD programmed cell death ricinosomes suspensor TUNEL Cell Death Cell Nucleus Chenopodium quinoa Cysteine Endopeptidases DNA Fragmentation Endosperm Flow Cytometry Organelles Subcellular Fractions
spellingShingle	Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot Cysteine endopeptidase Endosperm Micropylar endosperm PCD Programmed cell death Ricinosomes Suspensor TUNEL. abnormality bioassay biological development DNA enzyme activity germination immune response lipid maturation protein seed sperm cysteine proteinase Amaranthaceae apoptosis article cell death cell fractionation cell nucleus cell organelle Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cytology DNA fragmentation endosperm enzymology flow cytometry growth, development and aging metabolism micropylar endosperm nick end labeling PCD ricinosomes suspensor ultrastructure Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cysteine endopeptidase endosperm micropylar endosperm PCD programmed cell death ricinosomes suspensor TUNEL Cell Death Cell Nucleus Chenopodium quinoa Cysteine Endopeptidases DNA Fragmentation Endosperm Flow Cytometry Organelles Subcellular Fractions López-Fernández, M.P. Maldonado, S. Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa)
topic_facet	Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot Cysteine endopeptidase Endosperm Micropylar endosperm PCD Programmed cell death Ricinosomes Suspensor TUNEL. abnormality bioassay biological development DNA enzyme activity germination immune response lipid maturation protein seed sperm cysteine proteinase Amaranthaceae apoptosis article cell death cell fractionation cell nucleus cell organelle Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cytology DNA fragmentation endosperm enzymology flow cytometry growth, development and aging metabolism micropylar endosperm nick end labeling PCD ricinosomes suspensor ultrastructure Amaranthaceae Chenopodiaceae Chenopodium quinoa Cys-EP Cys-EP in situ immunolocalization Cys-EP western blot cysteine endopeptidase endosperm micropylar endosperm PCD programmed cell death ricinosomes suspensor TUNEL Cell Death Cell Nucleus Chenopodium quinoa Cysteine Endopeptidases DNA Fragmentation Endosperm Flow Cytometry Organelles Subcellular Fractions
description	Background and Aims In mature quinoa (Chenopodium quinoa) seeds, the lasting endosperm forms a micropylar cone covering the radicle. The suspensor cells lie within the centre of the cone. During the final stage of seed development, the cells of the lasting endosperm accumulate protein and lipids while the rest are crushed and disintegrated. Both the suspensor and endosperm die progressively from the innermost layers surrounding the embryo and extending towards the nucellar tissue. Ricinosomes are endoplasmic reticulum-derived organelles that accumulate both the pro-form and the mature form of cysteine endopeptidase (Cys-EP), first identified in castor bean (Ricinus communis) endosperm during germination. This study sought to identify associations between the presence of ricinosomes and programmed cell death (PCD) hallmarks in suspensor and endosperm cells predestined to die during quinoa seed development. MethodsAstructural study using light microscopy and transmission electron microscopywas performed. To detect the presence of Cys-EP, both western blot and in situ immunolocalization assays were carried out using anti- R. communis Cys-EP antibody. ATUNEL assay was used to determine DNA fragmentation. Results and Conclusions Except for the one or two cell layers that constitute the lasting endosperm in the mature seed, ricinosomes were found in suspensor and endosperm cells. These cells were also the site of morphological abnormalities, including misshapen and fragmented nuclei, vesiculation of the cytosol, vacuole collapse and cell wall disorganization. It is proposed that, in suspensor and endosperm cells, the early detection of Cys-EP in ricinosomes predicts the occurrence of PCD during late seed development. © The Author 2013.
format	JOUR
author	López-Fernández, M.P. Maldonado, S.
author_facet	López-Fernández, M.P. Maldonado, S.
author_sort	López-Fernández, M.P.
title	Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa)
title_short	Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa)
title_full	Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa)
title_fullStr	Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa)
title_full_unstemmed	Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa)
title_sort	ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (chenopodium quinoa)
url	http://hdl.handle.net/20.500.12110/paper_03057364_v112_n7_p1253_LopezFernandez
work_keys_str_mv	AT lopezfernandezmp ricinosomesprovideanearlyindicatorofsuspensorandendospermcellsdestinedtodieduringlateseeddevelopmentinquinoachenopodiumquinoa AT maldonados ricinosomesprovideanearlyindicatorofsuspensorandendospermcellsdestinedtodieduringlateseeddevelopmentinquinoachenopodiumquinoa
_version_	1807318748320759808

Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa)

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