Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis
1. 1. The effect of growing Euglena gracilis cells under light (photoheterotrophic) and dark (heterotrophic) conditions on growth, chlorophyll accumulation, ALA-S, DOVA-T activities and total soluble protein content was studied. 2. 2. Growth is greater in dark-grown cells. 3. 3. When cells previousl...
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todo:paper_03050491_v91_n2_p279_Lombardo2023-10-03T15:21:20Z Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis Lombardo, M.E. Araujo, L.S. Juknat, A.A. Del C. Batllet, A.M. 1. 1. The effect of growing Euglena gracilis cells under light (photoheterotrophic) and dark (heterotrophic) conditions on growth, chlorophyll accumulation, ALA-S, DOVA-T activities and total soluble protein content was studied. 2. 2. Growth is greater in dark-grown cells. 3. 3. When cells previously grown in dark are transferred to light, chlorophyll synthesis is rapidly induced, although maximal greening attained is lower than that reached when cells are grown photoheterotrophically from start. 4. 4. Ninety to ninety-five percent of total green pigment is chlorophyll α. Its increased formation with light parallels that of carotenoids. 5. 5. Both ALA-S and DOVA-T activities are greater in light-grown cells. When chloroplast development is light-induced in dark-preincubated cells shifted to light, the levels of both enzymes are also enhanced. 6. 6. Total soluble protein content of cells is greater in greening cultures and decreases with time. 7. 7. Independently of growing conditions, DOVA-T activity is always 10-15 times higher than that of ALA-S. © 1988. Fil:Lombardo, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Araujo, L.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Juknat, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03050491_v91_n2_p279_Lombardo |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| description |
1. 1. The effect of growing Euglena gracilis cells under light (photoheterotrophic) and dark (heterotrophic) conditions on growth, chlorophyll accumulation, ALA-S, DOVA-T activities and total soluble protein content was studied. 2. 2. Growth is greater in dark-grown cells. 3. 3. When cells previously grown in dark are transferred to light, chlorophyll synthesis is rapidly induced, although maximal greening attained is lower than that reached when cells are grown photoheterotrophically from start. 4. 4. Ninety to ninety-five percent of total green pigment is chlorophyll α. Its increased formation with light parallels that of carotenoids. 5. 5. Both ALA-S and DOVA-T activities are greater in light-grown cells. When chloroplast development is light-induced in dark-preincubated cells shifted to light, the levels of both enzymes are also enhanced. 6. 6. Total soluble protein content of cells is greater in greening cultures and decreases with time. 7. 7. Independently of growing conditions, DOVA-T activity is always 10-15 times higher than that of ALA-S. © 1988. |
| format |
JOUR |
| author |
Lombardo, M.E. Araujo, L.S. Juknat, A.A. Del C. Batllet, A.M. |
| spellingShingle |
Lombardo, M.E. Araujo, L.S. Juknat, A.A. Del C. Batllet, A.M. Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis |
| author_facet |
Lombardo, M.E. Araujo, L.S. Juknat, A.A. Del C. Batllet, A.M. |
| author_sort |
Lombardo, M.E. |
| title |
Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis |
| title_short |
Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis |
| title_full |
Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis |
| title_fullStr |
Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis |
| title_full_unstemmed |
Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis |
| title_sort |
effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in euglena gracilis |
| url |
http://hdl.handle.net/20.500.12110/paper_03050491_v91_n2_p279_Lombardo |
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