The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein

1. 1. The complex of glycoconjugates extracted with phenol-water from culture epimastigotes of a non-infective line (To) of Trypanosoma cruzi, derived from the Tulahuén strain showed only two components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. 2. The slower migrating glycocon...

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Autores principales: de Lederkremer, R.M., Waksman, N., de Martini, G.J.W., Segura, E.L.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03050491_v72_n2_p313_deLederkremer
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Sumario:1. 1. The complex of glycoconjugates extracted with phenol-water from culture epimastigotes of a non-infective line (To) of Trypanosoma cruzi, derived from the Tulahuén strain showed only two components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. 2. The slower migrating glycoconjugate, a glycoprotein of molecular weight 45,000 was purified to apparent homogeneity. 3. 3. A content of 76% protein, 17% neutral sugars and 6% glucosamine was analysed. Galactose and mannose occur in a ratio of 3:1 as determined by gas-liquid chromatography. A trace of glucose was detected in some samples. © 1982.