The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein

1. 1. The complex of glycoconjugates extracted with phenol-water from culture epimastigotes of a non-infective line (To) of Trypanosoma cruzi, derived from the Tulahuén strain showed only two components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. 2. The slower migrating glycocon...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: de Lederkremer, R.M., Waksman, N., de Martini, G.J.W., Segura, E.L.
Formato: JOUR
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03050491_v72_n2_p313_deLederkremer
Aporte de:
id todo:paper_03050491_v72_n2_p313_deLederkremer
record_format dspace
spelling todo:paper_03050491_v72_n2_p313_deLederkremer2023-10-03T15:21:15Z The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein de Lederkremer, R.M. Waksman, N. de Martini, G.J.W. Segura, E.L. carbohydrate glycoprotein animal article gas chromatography isolation and purification macromolecule molecular weight polyacrylamide gel electrophoresis Trypanosoma cruzi Animal Carbohydrates Chromatography, Gas Electrophoresis, Polyacrylamide Gel Glycoproteins Macromolecular Systems Molecular Weight Support, Non-U.S. Gov't Trypanosoma cruzi 1. 1. The complex of glycoconjugates extracted with phenol-water from culture epimastigotes of a non-infective line (To) of Trypanosoma cruzi, derived from the Tulahuén strain showed only two components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. 2. The slower migrating glycoconjugate, a glycoprotein of molecular weight 45,000 was purified to apparent homogeneity. 3. 3. A content of 76% protein, 17% neutral sugars and 6% glucosamine was analysed. Galactose and mannose occur in a ratio of 3:1 as determined by gas-liquid chromatography. A trace of glucose was detected in some samples. © 1982. Fil:de Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Waksman, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03050491_v72_n2_p313_deLederkremer
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic carbohydrate
glycoprotein
animal
article
gas chromatography
isolation and purification
macromolecule
molecular weight
polyacrylamide gel electrophoresis
Trypanosoma cruzi
Animal
Carbohydrates
Chromatography, Gas
Electrophoresis, Polyacrylamide Gel
Glycoproteins
Macromolecular Systems
Molecular Weight
Support, Non-U.S. Gov't
Trypanosoma cruzi
spellingShingle carbohydrate
glycoprotein
animal
article
gas chromatography
isolation and purification
macromolecule
molecular weight
polyacrylamide gel electrophoresis
Trypanosoma cruzi
Animal
Carbohydrates
Chromatography, Gas
Electrophoresis, Polyacrylamide Gel
Glycoproteins
Macromolecular Systems
Molecular Weight
Support, Non-U.S. Gov't
Trypanosoma cruzi
de Lederkremer, R.M.
Waksman, N.
de Martini, G.J.W.
Segura, E.L.
The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein
topic_facet carbohydrate
glycoprotein
animal
article
gas chromatography
isolation and purification
macromolecule
molecular weight
polyacrylamide gel electrophoresis
Trypanosoma cruzi
Animal
Carbohydrates
Chromatography, Gas
Electrophoresis, Polyacrylamide Gel
Glycoproteins
Macromolecular Systems
Molecular Weight
Support, Non-U.S. Gov't
Trypanosoma cruzi
description 1. 1. The complex of glycoconjugates extracted with phenol-water from culture epimastigotes of a non-infective line (To) of Trypanosoma cruzi, derived from the Tulahuén strain showed only two components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. 2. The slower migrating glycoconjugate, a glycoprotein of molecular weight 45,000 was purified to apparent homogeneity. 3. 3. A content of 76% protein, 17% neutral sugars and 6% glucosamine was analysed. Galactose and mannose occur in a ratio of 3:1 as determined by gas-liquid chromatography. A trace of glucose was detected in some samples. © 1982.
format JOUR
author de Lederkremer, R.M.
Waksman, N.
de Martini, G.J.W.
Segura, E.L.
author_facet de Lederkremer, R.M.
Waksman, N.
de Martini, G.J.W.
Segura, E.L.
author_sort de Lederkremer, R.M.
title The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein
title_short The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein
title_full The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein
title_fullStr The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein
title_full_unstemmed The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein
title_sort glycoconjugates from trypanosoma cruzi, purification and composition of a glycoprotein
url http://hdl.handle.net/20.500.12110/paper_03050491_v72_n2_p313_deLederkremer
work_keys_str_mv AT delederkremerrm theglycoconjugatesfromtrypanosomacruzipurificationandcompositionofaglycoprotein
AT waksmann theglycoconjugatesfromtrypanosomacruzipurificationandcompositionofaglycoprotein
AT demartinigjw theglycoconjugatesfromtrypanosomacruzipurificationandcompositionofaglycoprotein
AT segurael theglycoconjugatesfromtrypanosomacruzipurificationandcompositionofaglycoprotein
AT delederkremerrm glycoconjugatesfromtrypanosomacruzipurificationandcompositionofaglycoprotein
AT waksmann glycoconjugatesfromtrypanosomacruzipurificationandcompositionofaglycoprotein
AT demartinigjw glycoconjugatesfromtrypanosomacruzipurificationandcompositionofaglycoprotein
AT segurael glycoconjugatesfromtrypanosomacruzipurificationandcompositionofaglycoprotein
_version_ 1807318472395325440