The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein
1. 1. The complex of glycoconjugates extracted with phenol-water from culture epimastigotes of a non-infective line (To) of Trypanosoma cruzi, derived from the Tulahuén strain showed only two components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. 2. The slower migrating glycocon...
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todo:paper_03050491_v72_n2_p313_deLederkremer2023-10-03T15:21:15Z The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein de Lederkremer, R.M. Waksman, N. de Martini, G.J.W. Segura, E.L. carbohydrate glycoprotein animal article gas chromatography isolation and purification macromolecule molecular weight polyacrylamide gel electrophoresis Trypanosoma cruzi Animal Carbohydrates Chromatography, Gas Electrophoresis, Polyacrylamide Gel Glycoproteins Macromolecular Systems Molecular Weight Support, Non-U.S. Gov't Trypanosoma cruzi 1. 1. The complex of glycoconjugates extracted with phenol-water from culture epimastigotes of a non-infective line (To) of Trypanosoma cruzi, derived from the Tulahuén strain showed only two components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. 2. The slower migrating glycoconjugate, a glycoprotein of molecular weight 45,000 was purified to apparent homogeneity. 3. 3. A content of 76% protein, 17% neutral sugars and 6% glucosamine was analysed. Galactose and mannose occur in a ratio of 3:1 as determined by gas-liquid chromatography. A trace of glucose was detected in some samples. © 1982. Fil:de Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Waksman, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03050491_v72_n2_p313_deLederkremer |
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Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
carbohydrate glycoprotein animal article gas chromatography isolation and purification macromolecule molecular weight polyacrylamide gel electrophoresis Trypanosoma cruzi Animal Carbohydrates Chromatography, Gas Electrophoresis, Polyacrylamide Gel Glycoproteins Macromolecular Systems Molecular Weight Support, Non-U.S. Gov't Trypanosoma cruzi |
spellingShingle |
carbohydrate glycoprotein animal article gas chromatography isolation and purification macromolecule molecular weight polyacrylamide gel electrophoresis Trypanosoma cruzi Animal Carbohydrates Chromatography, Gas Electrophoresis, Polyacrylamide Gel Glycoproteins Macromolecular Systems Molecular Weight Support, Non-U.S. Gov't Trypanosoma cruzi de Lederkremer, R.M. Waksman, N. de Martini, G.J.W. Segura, E.L. The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein |
topic_facet |
carbohydrate glycoprotein animal article gas chromatography isolation and purification macromolecule molecular weight polyacrylamide gel electrophoresis Trypanosoma cruzi Animal Carbohydrates Chromatography, Gas Electrophoresis, Polyacrylamide Gel Glycoproteins Macromolecular Systems Molecular Weight Support, Non-U.S. Gov't Trypanosoma cruzi |
description |
1. 1. The complex of glycoconjugates extracted with phenol-water from culture epimastigotes of a non-infective line (To) of Trypanosoma cruzi, derived from the Tulahuén strain showed only two components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. 2. The slower migrating glycoconjugate, a glycoprotein of molecular weight 45,000 was purified to apparent homogeneity. 3. 3. A content of 76% protein, 17% neutral sugars and 6% glucosamine was analysed. Galactose and mannose occur in a ratio of 3:1 as determined by gas-liquid chromatography. A trace of glucose was detected in some samples. © 1982. |
format |
JOUR |
author |
de Lederkremer, R.M. Waksman, N. de Martini, G.J.W. Segura, E.L. |
author_facet |
de Lederkremer, R.M. Waksman, N. de Martini, G.J.W. Segura, E.L. |
author_sort |
de Lederkremer, R.M. |
title |
The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein |
title_short |
The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein |
title_full |
The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein |
title_fullStr |
The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein |
title_full_unstemmed |
The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein |
title_sort |
glycoconjugates from trypanosoma cruzi, purification and composition of a glycoprotein |
url |
http://hdl.handle.net/20.500.12110/paper_03050491_v72_n2_p313_deLederkremer |
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