Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype

Heme biosynthesis was studied in the segregants of Saccharomyces cerevisiae (DW10 tetrade 2) from D27 and D27/C6 mating, as a function of the carbon source in the growth medium and the physiological state of the cells. The effects of the HEM R+ gene on the 5-aminolevulinate synthase (ALA-S) and 5-am...

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Autores principales:	De Siervi, A., Rossetti, M.V., Lezama, D., Batlle, A.M.D.C.
Formato:	JOUR
Materias:	5-aminolevulinate dehydratase 5-aminolevulinate synthase catabolite repression and de-repression HEM R+ mutants heme biosynthesis intracellular 5-aminolevulinate acid porphobilinogen-deaminase Saccharomyces cerevisiae heme article heme synthesis mutant phenotype priority journal
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03050491_v115_n2_p167_DeSiervi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03050491_v115_n2_p167_DeSiervi
record_format	dspace
spelling	todo:paper_03050491_v115_n2_p167_DeSiervi2023-10-03T15:21:14Z Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype De Siervi, A. Rossetti, M.V. Lezama, D. Batlle, A.M.D.C. 5-aminolevulinate dehydratase 5-aminolevulinate synthase catabolite repression and de-repression HEM R+ mutants heme biosynthesis intracellular 5-aminolevulinate acid porphobilinogen-deaminase Saccharomyces cerevisiae heme article heme synthesis mutant phenotype priority journal Saccharomyces cerevisiae Saccharomyces cerevisiae Heme biosynthesis was studied in the segregants of Saccharomyces cerevisiae (DW10 tetrade 2) from D27 and D27/C6 mating, as a function of the carbon source in the growth medium and the physiological state of the cells. The effects of the HEM R+ gene on the 5-aminolevulinate synthase (ALA-S) and 5-aminolevulinate dehydratase (ALA-D) activities of heme biosynthesis in cells grown on nonfermentable and fermentable carbon sources were compared. Profiles obtained for both strains grown on a fermentable carbon source (glucose) were identical. However, in the presence of a nonfermentable carbon source (ethanol), they behave quite differently, as if the mutation could only be expressed under these growth conditions. Moreover, their behavior is similar to that found for the parental strains, indicating that for the mutant its particular behavior might be inheritedly linked to the HEM R+ gene, which in turn affects some regulatory aspects of ALA synthesis explaining its characteristic phenotype. Fil:De Siervi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A.M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03050491_v115_n2_p167_DeSiervi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	5-aminolevulinate dehydratase 5-aminolevulinate synthase catabolite repression and de-repression HEM R+ mutants heme biosynthesis intracellular 5-aminolevulinate acid porphobilinogen-deaminase Saccharomyces cerevisiae heme article heme synthesis mutant phenotype priority journal Saccharomyces cerevisiae Saccharomyces cerevisiae
spellingShingle	5-aminolevulinate dehydratase 5-aminolevulinate synthase catabolite repression and de-repression HEM R+ mutants heme biosynthesis intracellular 5-aminolevulinate acid porphobilinogen-deaminase Saccharomyces cerevisiae heme article heme synthesis mutant phenotype priority journal Saccharomyces cerevisiae Saccharomyces cerevisiae De Siervi, A. Rossetti, M.V. Lezama, D. Batlle, A.M.D.C. Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype
topic_facet	5-aminolevulinate dehydratase 5-aminolevulinate synthase catabolite repression and de-repression HEM R+ mutants heme biosynthesis intracellular 5-aminolevulinate acid porphobilinogen-deaminase Saccharomyces cerevisiae heme article heme synthesis mutant phenotype priority journal Saccharomyces cerevisiae Saccharomyces cerevisiae
description	Heme biosynthesis was studied in the segregants of Saccharomyces cerevisiae (DW10 tetrade 2) from D27 and D27/C6 mating, as a function of the carbon source in the growth medium and the physiological state of the cells. The effects of the HEM R+ gene on the 5-aminolevulinate synthase (ALA-S) and 5-aminolevulinate dehydratase (ALA-D) activities of heme biosynthesis in cells grown on nonfermentable and fermentable carbon sources were compared. Profiles obtained for both strains grown on a fermentable carbon source (glucose) were identical. However, in the presence of a nonfermentable carbon source (ethanol), they behave quite differently, as if the mutation could only be expressed under these growth conditions. Moreover, their behavior is similar to that found for the parental strains, indicating that for the mutant its particular behavior might be inheritedly linked to the HEM R+ gene, which in turn affects some regulatory aspects of ALA synthesis explaining its characteristic phenotype.
format	JOUR
author	De Siervi, A. Rossetti, M.V. Lezama, D. Batlle, A.M.D.C.
author_facet	De Siervi, A. Rossetti, M.V. Lezama, D. Batlle, A.M.D.C.
author_sort	De Siervi, A.
title	Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype
title_short	Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype
title_full	Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype
title_fullStr	Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype
title_full_unstemmed	Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype
title_sort	porphyrin biosynthesis in normal and haem mutants of saccharomyces cerevisiae. studies on the inheritance of the hem r+ phenotype
url	http://hdl.handle.net/20.500.12110/paper_03050491_v115_n2_p167_DeSiervi
work_keys_str_mv	AT desiervia porphyrinbiosynthesisinnormalandhaemmutantsofsaccharomycescerevisiaestudiesontheinheritanceofthehemrphenotype AT rossettimv porphyrinbiosynthesisinnormalandhaemmutantsofsaccharomycescerevisiaestudiesontheinheritanceofthehemrphenotype AT lezamad porphyrinbiosynthesisinnormalandhaemmutantsofsaccharomycescerevisiaestudiesontheinheritanceofthehemrphenotype AT batlleamdc porphyrinbiosynthesisinnormalandhaemmutantsofsaccharomycescerevisiaestudiesontheinheritanceofthehemrphenotype
_version_	1782025026782887936

Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype

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