Resistance to superinfection of Vero cells persistently infected with Junin virus

Two non-virogenic Vero cell lines persistently infected with the arenavirus Junin (JUNV), named V3 and V7, were characterized with respect to their resistance to superinfection with homologous and antigenically related viruses. Both lines were refractory to JUNV multiplication and partially resistan...

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Autores principales:	Ellenberg, P., Edreira, M., Scolaro, L.
Formato:	JOUR
Materias:	nucleocapsid protein animal article cell line Cercopithecus genetics giant cell hamster heat Junin virus metabolism pathogenicity physiology superinfection Vero cell virology virus culture virus interference virus replication Animals Cell Line Cercopithecus aethiops Cricetinae Giant Cells Heat Nucleocapsid Proteins Plaque Assay Superinfection Vero Cells Viral Interference Virus Replication Animalia Arenavirus RNA viruses
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03048608_v149_n3_p507_Ellenberg
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_03048608_v149_n3_p507_Ellenberg2023-10-03T15:20:58Z Resistance to superinfection of Vero cells persistently infected with Junin virus Ellenberg, P. Edreira, M. Scolaro, L. nucleocapsid protein animal article cell line Cercopithecus genetics giant cell hamster heat Junin virus metabolism pathogenicity physiology superinfection Vero cell virology virus culture virus interference virus replication Animals Cell Line Cercopithecus aethiops Cricetinae Giant Cells Heat Junin virus Nucleocapsid Proteins Plaque Assay Superinfection Vero Cells Viral Interference Virus Replication Animalia Arenavirus Cercopithecus Cricetinae Junin virus RNA viruses Two non-virogenic Vero cell lines persistently infected with the arenavirus Junin (JUNV), named V3 and V7, were characterized with respect to their resistance to superinfection with homologous and antigenically related viruses. Both lines were refractory to JUNV multiplication and partially resistant to other arenaviruses. JUNV was able to adsorb and penetrate persistently infected cells and, although V3 and V7 were able to support synthesis of antigenomic sense viral RNA, protein production of superinfecting virus was totally blocked. This resistance was not mediated by defective interfering particles but rather by a "cell-associated factor" that could be cell to cell transmitted. Prolonged thermal treatment of V3 and V7 abrogated expression of the viral nucleoprotein (N) and turned persistently infected cells permissive to JUNV multiplication. Thermal treated cells cultured at 37°C resumed the expression of N in association to the recovery of resistance. Results strongly suggest a correlation between the presence of the viral nucleoprotein and superinfection exclusion. Fil:Ellenberg, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Edreira, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scolaro, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03048608_v149_n3_p507_Ellenberg
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	nucleocapsid protein animal article cell line Cercopithecus genetics giant cell hamster heat Junin virus metabolism pathogenicity physiology superinfection Vero cell virology virus culture virus interference virus replication Animals Cell Line Cercopithecus aethiops Cricetinae Giant Cells Heat Junin virus Nucleocapsid Proteins Plaque Assay Superinfection Vero Cells Viral Interference Virus Replication Animalia Arenavirus Cercopithecus Cricetinae Junin virus RNA viruses
spellingShingle	nucleocapsid protein animal article cell line Cercopithecus genetics giant cell hamster heat Junin virus metabolism pathogenicity physiology superinfection Vero cell virology virus culture virus interference virus replication Animals Cell Line Cercopithecus aethiops Cricetinae Giant Cells Heat Junin virus Nucleocapsid Proteins Plaque Assay Superinfection Vero Cells Viral Interference Virus Replication Animalia Arenavirus Cercopithecus Cricetinae Junin virus RNA viruses Ellenberg, P. Edreira, M. Scolaro, L. Resistance to superinfection of Vero cells persistently infected with Junin virus
topic_facet	nucleocapsid protein animal article cell line Cercopithecus genetics giant cell hamster heat Junin virus metabolism pathogenicity physiology superinfection Vero cell virology virus culture virus interference virus replication Animals Cell Line Cercopithecus aethiops Cricetinae Giant Cells Heat Junin virus Nucleocapsid Proteins Plaque Assay Superinfection Vero Cells Viral Interference Virus Replication Animalia Arenavirus Cercopithecus Cricetinae Junin virus RNA viruses
description	Two non-virogenic Vero cell lines persistently infected with the arenavirus Junin (JUNV), named V3 and V7, were characterized with respect to their resistance to superinfection with homologous and antigenically related viruses. Both lines were refractory to JUNV multiplication and partially resistant to other arenaviruses. JUNV was able to adsorb and penetrate persistently infected cells and, although V3 and V7 were able to support synthesis of antigenomic sense viral RNA, protein production of superinfecting virus was totally blocked. This resistance was not mediated by defective interfering particles but rather by a "cell-associated factor" that could be cell to cell transmitted. Prolonged thermal treatment of V3 and V7 abrogated expression of the viral nucleoprotein (N) and turned persistently infected cells permissive to JUNV multiplication. Thermal treated cells cultured at 37°C resumed the expression of N in association to the recovery of resistance. Results strongly suggest a correlation between the presence of the viral nucleoprotein and superinfection exclusion.
format	JOUR
author	Ellenberg, P. Edreira, M. Scolaro, L.
author_facet	Ellenberg, P. Edreira, M. Scolaro, L.
author_sort	Ellenberg, P.
title	Resistance to superinfection of Vero cells persistently infected with Junin virus
title_short	Resistance to superinfection of Vero cells persistently infected with Junin virus
title_full	Resistance to superinfection of Vero cells persistently infected with Junin virus
title_fullStr	Resistance to superinfection of Vero cells persistently infected with Junin virus
title_full_unstemmed	Resistance to superinfection of Vero cells persistently infected with Junin virus
title_sort	resistance to superinfection of vero cells persistently infected with junin virus
url	http://hdl.handle.net/20.500.12110/paper_03048608_v149_n3_p507_Ellenberg
work_keys_str_mv	AT ellenbergp resistancetosuperinfectionofverocellspersistentlyinfectedwithjuninvirus AT edreiram resistancetosuperinfectionofverocellspersistentlyinfectedwithjuninvirus AT scolarol resistancetosuperinfectionofverocellspersistentlyinfectedwithjuninvirus
_version_	1807322166724657152

Resistance to superinfection of Vero cells persistently infected with Junin virus

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