Involvement of vacuolar proton ATPase in Junin virus multiplication

The role of vacuolar-proton ATPase (V-H+ATPAse) on Junin virus (JV) replication was evaluated by analyzing the effect of specific inhibitors of the enzyme activity on different steps of virus multiplication cycle. The presence of the macrolide antibiotics bafilomycin A1 and concanamycin A during the...

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Autores principales: Castilla, V., Palermo, L.M., Coto, C.E.
Formato: JOUR
Materias:
bafilomycin
cell adhesion
cell vacuole
concanamycin A
endocytosis
enzyme activity
enzyme inhibition
Junin virus
proton transporting adenosine triphosphatase
virus enzyme
virus infection
virus replication
Adsorption
Animals
Anti-Bacterial Agents
Cell Line
Cell Survival
Cercopithecus aethiops
Cricetinae
Enzyme Inhibitors
Fluorescent Antibody Technique, Indirect
Macrolides
Protein Transport
Proton-Translocating ATPases
Vacuolar Proton-Translocating ATPases
Vero Cells
Viral Proteins
Virus Replication
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03048608_v146_n2_p251_Castilla
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03048608_v146_n2_p251_Castilla
record_format dspace
spelling todo:paper_03048608_v146_n2_p251_Castilla2023-10-03T15:20:57Z Involvement of vacuolar proton ATPase in Junin virus multiplication Castilla, V. Palermo, L.M. Coto, C.E. bafilomycin cell adhesion cell vacuole concanamycin A endocytosis enzyme activity enzyme inhibition Junin virus proton transporting adenosine triphosphatase virus enzyme virus infection virus replication Adsorption Animals Anti-Bacterial Agents Cell Line Cell Survival Cercopithecus aethiops Cricetinae Enzyme Inhibitors Fluorescent Antibody Technique, Indirect Junin virus Macrolides Protein Transport Proton-Translocating ATPases Vacuolar Proton-Translocating ATPases Vero Cells Viral Proteins Virus Replication Junin virus The role of vacuolar-proton ATPase (V-H+ATPAse) on Junin virus (JV) replication was evaluated by analyzing the effect of specific inhibitors of the enzyme activity on different steps of virus multiplication cycle. The presence of the macrolide antibiotics bafilomycin A1 and concanamycin A during the first two hours of infection caused a significant reduction of extracellular infectious virus production and viral protein expression in Vero and BHK-21 cells. The inhibitory action of the compounds was mainly exerted at an early stage of the JV multiplication cycle, without affecting virus attachment to the cell but preventing virus penetration. A correlation between the inhibitory action of the compounds on intracellular compartments acidification and the reduction of JV yield was observed. The addition of concanamycin A at different times after infection indicated that the compound also interferes with the release of infectious particles to the extracellular medium. Although, intracellular transport of JV glycoproteins to the cell membrane, seems not to be affected as revealed by immunofluorescence staining. The results confirm that JV enters into the cell through the endocytic pathway as previously suggested by using lysosomotropic compounds. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Palermo, L.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03048608_v146_n2_p251_Castilla
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic bafilomycin
cell adhesion
cell vacuole
concanamycin A
endocytosis
enzyme activity
enzyme inhibition
Junin virus
proton transporting adenosine triphosphatase
virus enzyme
virus infection
virus replication
Adsorption
Animals
Anti-Bacterial Agents
Cell Line
Cell Survival
Cercopithecus aethiops
Cricetinae
Enzyme Inhibitors
Fluorescent Antibody Technique, Indirect
Junin virus
Macrolides
Protein Transport
Proton-Translocating ATPases
Vacuolar Proton-Translocating ATPases
Vero Cells
Viral Proteins
Virus Replication
Junin virus
spellingShingle bafilomycin
cell adhesion
cell vacuole
concanamycin A
endocytosis
enzyme activity
enzyme inhibition
Junin virus
proton transporting adenosine triphosphatase
virus enzyme
virus infection
virus replication
Adsorption
Animals
Anti-Bacterial Agents
Cell Line
Cell Survival
Cercopithecus aethiops
Cricetinae
Enzyme Inhibitors
Fluorescent Antibody Technique, Indirect
Junin virus
Macrolides
Protein Transport
Proton-Translocating ATPases
Vacuolar Proton-Translocating ATPases
Vero Cells
Viral Proteins
Virus Replication
Junin virus
Castilla, V.
Palermo, L.M.
Coto, C.E.
Involvement of vacuolar proton ATPase in Junin virus multiplication
topic_facet bafilomycin
cell adhesion
cell vacuole
concanamycin A
endocytosis
enzyme activity
enzyme inhibition
Junin virus
proton transporting adenosine triphosphatase
virus enzyme
virus infection
virus replication
Adsorption
Animals
Anti-Bacterial Agents
Cell Line
Cell Survival
Cercopithecus aethiops
Cricetinae
Enzyme Inhibitors
Fluorescent Antibody Technique, Indirect
Junin virus
Macrolides
Protein Transport
Proton-Translocating ATPases
Vacuolar Proton-Translocating ATPases
Vero Cells
Viral Proteins
Virus Replication
Junin virus
description The role of vacuolar-proton ATPase (V-H+ATPAse) on Junin virus (JV) replication was evaluated by analyzing the effect of specific inhibitors of the enzyme activity on different steps of virus multiplication cycle. The presence of the macrolide antibiotics bafilomycin A1 and concanamycin A during the first two hours of infection caused a significant reduction of extracellular infectious virus production and viral protein expression in Vero and BHK-21 cells. The inhibitory action of the compounds was mainly exerted at an early stage of the JV multiplication cycle, without affecting virus attachment to the cell but preventing virus penetration. A correlation between the inhibitory action of the compounds on intracellular compartments acidification and the reduction of JV yield was observed. The addition of concanamycin A at different times after infection indicated that the compound also interferes with the release of infectious particles to the extracellular medium. Although, intracellular transport of JV glycoproteins to the cell membrane, seems not to be affected as revealed by immunofluorescence staining. The results confirm that JV enters into the cell through the endocytic pathway as previously suggested by using lysosomotropic compounds.
format JOUR
author Castilla, V.
Palermo, L.M.
Coto, C.E.
author_facet Castilla, V.
Palermo, L.M.
Coto, C.E.
author_sort Castilla, V.
title Involvement of vacuolar proton ATPase in Junin virus multiplication
title_short Involvement of vacuolar proton ATPase in Junin virus multiplication
title_full Involvement of vacuolar proton ATPase in Junin virus multiplication
title_fullStr Involvement of vacuolar proton ATPase in Junin virus multiplication
title_full_unstemmed Involvement of vacuolar proton ATPase in Junin virus multiplication
title_sort involvement of vacuolar proton atpase in junin virus multiplication
url http://hdl.handle.net/20.500.12110/paper_03048608_v146_n2_p251_Castilla
work_keys_str_mv AT castillav involvementofvacuolarprotonatpaseinjuninvirusmultiplication
AT palermolm involvementofvacuolarprotonatpaseinjuninvirusmultiplication
AT cotoce involvementofvacuolarprotonatpaseinjuninvirusmultiplication
_version_ 1782028716892749824

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