Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV)

Partial genomic sequences from an unknown garlic potyvirus and from an onion isolate of the onion yellow dwarf potyvirus (OYDV) were obtained. Comparison of the deduced amine acid sequences showed it similarity of 88% between the respective vital coat proteins. The garlic potyvirus coat protein was...

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Autores principales: Kobayashi, K., Rabinowicz, P., Bravo-Almonacid, F., Helguera, M., Conci, V., Lot, H., Mentaberry, A.
Formato: JOUR
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03048608_v141_n12_p2277_Kobayashi
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_03048608_v141_n12_p2277_Kobayashi2023-10-03T15:20:55Z Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV) Kobayashi, K. Rabinowicz, P. Bravo-Almonacid, F. Helguera, M. Conci, V. Lot, H. Mentaberry, A. Partial genomic sequences from an unknown garlic potyvirus and from an onion isolate of the onion yellow dwarf potyvirus (OYDV) were obtained. Comparison of the deduced amine acid sequences showed it similarity of 88% between the respective vital coat proteins. The garlic potyvirus coat protein was expressed in E. coli cells, purified, and subjected to Western blot analysis using antibodies raised against different garlic-infecting viruses. The expression protein was consistently recognised by anti-OYDV antibodies and did not react with antibodies specific for leek yellow stripe potyvirus (LYSV), garlic common latent carlavirus (GCLV) and shallot latent carlavirus (SLV). Besides, the garlic potyvirus coat protein was obtained as a fusion protein and used as antigen to produce polyclonal antibodies. These antibodies reacted with purified OYDV virions, but failed to recognise LYSV particles. In the light of this evidence the garlic potyvirus was identified as the garlic strain of OYDV. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03048608_v141_n12_p2277_Kobayashi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description Partial genomic sequences from an unknown garlic potyvirus and from an onion isolate of the onion yellow dwarf potyvirus (OYDV) were obtained. Comparison of the deduced amine acid sequences showed it similarity of 88% between the respective vital coat proteins. The garlic potyvirus coat protein was expressed in E. coli cells, purified, and subjected to Western blot analysis using antibodies raised against different garlic-infecting viruses. The expression protein was consistently recognised by anti-OYDV antibodies and did not react with antibodies specific for leek yellow stripe potyvirus (LYSV), garlic common latent carlavirus (GCLV) and shallot latent carlavirus (SLV). Besides, the garlic potyvirus coat protein was obtained as a fusion protein and used as antigen to produce polyclonal antibodies. These antibodies reacted with purified OYDV virions, but failed to recognise LYSV particles. In the light of this evidence the garlic potyvirus was identified as the garlic strain of OYDV.
format JOUR
author Kobayashi, K.
Rabinowicz, P.
Bravo-Almonacid, F.
Helguera, M.
Conci, V.
Lot, H.
Mentaberry, A.
spellingShingle Kobayashi, K.
Rabinowicz, P.
Bravo-Almonacid, F.
Helguera, M.
Conci, V.
Lot, H.
Mentaberry, A.
Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV)
author_facet Kobayashi, K.
Rabinowicz, P.
Bravo-Almonacid, F.
Helguera, M.
Conci, V.
Lot, H.
Mentaberry, A.
author_sort Kobayashi, K.
title Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV)
title_short Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV)
title_full Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV)
title_fullStr Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV)
title_full_unstemmed Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV)
title_sort coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (oydv)
url http://hdl.handle.net/20.500.12110/paper_03048608_v141_n12_p2277_Kobayashi
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AT bravoalmonacidf coatproteingenesequencesofgarlicandonionisolatesoftheonionyellowdwarfpotyvirusoydv
AT helgueram coatproteingenesequencesofgarlicandonionisolatesoftheonionyellowdwarfpotyvirusoydv
AT conciv coatproteingenesequencesofgarlicandonionisolatesoftheonionyellowdwarfpotyvirusoydv
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AT mentaberrya coatproteingenesequencesofgarlicandonionisolatesoftheonionyellowdwarfpotyvirusoydv
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