Effect of 1,5-gluconolactone on the formation of a glucoprotein in potato tuber
The occurrence of a two-step mechanism for the synthesis of a protein bound glucan in potato tuber was confirmed based on a different behaviour of the engaged enzymes towards 1,5-D-gluconolactone. The absence of [14C]erythritol after Smith periodate degradation of the labelled product synthesized du...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03044211_v15_n2_p151_Tandecarz |
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todo:paper_03044211_v15_n2_p151_Tandecarz2023-10-03T15:20:53Z Effect of 1,5-gluconolactone on the formation of a glucoprotein in potato tuber Tandecarz, J.S. Cardini, C.E. The occurrence of a two-step mechanism for the synthesis of a protein bound glucan in potato tuber was confirmed based on a different behaviour of the engaged enzymes towards 1,5-D-gluconolactone. The absence of [14C]erythritol after Smith periodate degradation of the labelled product synthesized during both steps in the presence of 1,5-D-gluconolactone indicated a lack of 1,4 glucosidic linkages. On the contrary, the first step of the reaction, i.e., the formation of a peptidyl-glucose linkage, was not affected by the lactone. These results reinforce the hypothesis of a common mechanism for glucan chain elongation. © 1979. Fil:Tandecarz, J.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03044211_v15_n2_p151_Tandecarz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| description |
The occurrence of a two-step mechanism for the synthesis of a protein bound glucan in potato tuber was confirmed based on a different behaviour of the engaged enzymes towards 1,5-D-gluconolactone. The absence of [14C]erythritol after Smith periodate degradation of the labelled product synthesized during both steps in the presence of 1,5-D-gluconolactone indicated a lack of 1,4 glucosidic linkages. On the contrary, the first step of the reaction, i.e., the formation of a peptidyl-glucose linkage, was not affected by the lactone. These results reinforce the hypothesis of a common mechanism for glucan chain elongation. © 1979. |
| format |
JOUR |
| author |
Tandecarz, J.S. Cardini, C.E. |
| spellingShingle |
Tandecarz, J.S. Cardini, C.E. Effect of 1,5-gluconolactone on the formation of a glucoprotein in potato tuber |
| author_facet |
Tandecarz, J.S. Cardini, C.E. |
| author_sort |
Tandecarz, J.S. |
| title |
Effect of 1,5-gluconolactone on the formation of a glucoprotein in potato tuber |
| title_short |
Effect of 1,5-gluconolactone on the formation of a glucoprotein in potato tuber |
| title_full |
Effect of 1,5-gluconolactone on the formation of a glucoprotein in potato tuber |
| title_fullStr |
Effect of 1,5-gluconolactone on the formation of a glucoprotein in potato tuber |
| title_full_unstemmed |
Effect of 1,5-gluconolactone on the formation of a glucoprotein in potato tuber |
| title_sort |
effect of 1,5-gluconolactone on the formation of a glucoprotein in potato tuber |
| url |
http://hdl.handle.net/20.500.12110/paper_03044211_v15_n2_p151_Tandecarz |
| work_keys_str_mv |
AT tandecarzjs effectof15gluconolactoneontheformationofaglucoproteininpotatotuber AT cardinice effectof15gluconolactoneontheformationofaglucoproteininpotatotuber |
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1807323652208721920 |