Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase
1. 1. Further evidence for the formation of pyrrolic intermediates resulting from action of soybean callus porphobilinogenase on porphobilinogen in the absence or presence of ammonia, under anaerobic conditions, is reported. 2. 2. It was found that the activity of porphobilinogenase was affected by...
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todo:paper_03044165_v252_n3_p481_Stella2023-10-03T15:20:47Z Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase Stella, A.M. Parera, V.E. Llambías, E.B.C. Del C. Batlle, A.M. ammonia ammonium sulfate carbon hydrolase isomerase lyase oxygen porphyrin propionic acid derivative pyrrole derivative anaerobic growth article enzymology gel chromatography kinetics plant stereoisomerism synthesis thin layer chromatography Aminohydrolases Ammonia Ammonium Sulfate Anaerobiosis Carbon Isotopes Chromatography, Gel Chromatography, Thin Layer Isomerases Kinetics Lyases Oxygen Plants Porphyrins Propionates Pyrroles Stereoisomerism 1. 1. Further evidence for the formation of pyrrolic intermediates resulting from action of soybean callus porphobilinogenase on porphobilinogen in the absence or presence of ammonia, under anaerobic conditions, is reported. 2. 2. It was found that the activity of porphobilinogenase was affected by the presence of air; the yield of uroporphyrinogens was very low, although consumption of substrate was not greatly modified. These results suggested that some intermediate in the reaction was still being formed, but that this compound must be in the reduced form to be further converted into uroporphyrinogens. It was observed that ammonia probably produced as accumulation of pyrryl intermediates, but that they synthesize uroporphyrinogen III at a slower rate. 3. 3. Attempts were made to detect the formation of an enzyme-substrate complex, but they all failed. © 1971. Fil:Stella, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Parera, V.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Llambías, E.B.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03044165_v252_n3_p481_Stella |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
ammonia ammonium sulfate carbon hydrolase isomerase lyase oxygen porphyrin propionic acid derivative pyrrole derivative anaerobic growth article enzymology gel chromatography kinetics plant stereoisomerism synthesis thin layer chromatography Aminohydrolases Ammonia Ammonium Sulfate Anaerobiosis Carbon Isotopes Chromatography, Gel Chromatography, Thin Layer Isomerases Kinetics Lyases Oxygen Plants Porphyrins Propionates Pyrroles Stereoisomerism |
| spellingShingle |
ammonia ammonium sulfate carbon hydrolase isomerase lyase oxygen porphyrin propionic acid derivative pyrrole derivative anaerobic growth article enzymology gel chromatography kinetics plant stereoisomerism synthesis thin layer chromatography Aminohydrolases Ammonia Ammonium Sulfate Anaerobiosis Carbon Isotopes Chromatography, Gel Chromatography, Thin Layer Isomerases Kinetics Lyases Oxygen Plants Porphyrins Propionates Pyrroles Stereoisomerism Stella, A.M. Parera, V.E. Llambías, E.B.C. Del C. Batlle, A.M. Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase |
| topic_facet |
ammonia ammonium sulfate carbon hydrolase isomerase lyase oxygen porphyrin propionic acid derivative pyrrole derivative anaerobic growth article enzymology gel chromatography kinetics plant stereoisomerism synthesis thin layer chromatography Aminohydrolases Ammonia Ammonium Sulfate Anaerobiosis Carbon Isotopes Chromatography, Gel Chromatography, Thin Layer Isomerases Kinetics Lyases Oxygen Plants Porphyrins Propionates Pyrroles Stereoisomerism |
| description |
1. 1. Further evidence for the formation of pyrrolic intermediates resulting from action of soybean callus porphobilinogenase on porphobilinogen in the absence or presence of ammonia, under anaerobic conditions, is reported. 2. 2. It was found that the activity of porphobilinogenase was affected by the presence of air; the yield of uroporphyrinogens was very low, although consumption of substrate was not greatly modified. These results suggested that some intermediate in the reaction was still being formed, but that this compound must be in the reduced form to be further converted into uroporphyrinogens. It was observed that ammonia probably produced as accumulation of pyrryl intermediates, but that they synthesize uroporphyrinogen III at a slower rate. 3. 3. Attempts were made to detect the formation of an enzyme-substrate complex, but they all failed. © 1971. |
| format |
JOUR |
| author |
Stella, A.M. Parera, V.E. Llambías, E.B.C. Del C. Batlle, A.M. |
| author_facet |
Stella, A.M. Parera, V.E. Llambías, E.B.C. Del C. Batlle, A.M. |
| author_sort |
Stella, A.M. |
| title |
Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase |
| title_short |
Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase |
| title_full |
Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase |
| title_fullStr |
Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase |
| title_full_unstemmed |
Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase |
| title_sort |
pyrrylmethane intermediates in the synthesis of uroporphirigonen iii by soybean callus porphobilinogenase |
| url |
http://hdl.handle.net/20.500.12110/paper_03044165_v252_n3_p481_Stella |
| work_keys_str_mv |
AT stellaam pyrrylmethaneintermediatesinthesynthesisofuroporphirigoneniiibysoybeancallusporphobilinogenase AT parerave pyrrylmethaneintermediatesinthesynthesisofuroporphirigoneniiibysoybeancallusporphobilinogenase AT llambiasebc pyrrylmethaneintermediatesinthesynthesisofuroporphirigoneniiibysoybeancallusporphobilinogenase AT delcbatlleam pyrrylmethaneintermediatesinthesynthesisofuroporphirigoneniiibysoybeancallusporphobilinogenase |
| _version_ |
1782030660999839744 |