Combined effects of trehalose and cations on the thermal resistance of β-galactosidase in freeze-dried systems
The purpose of this study was to investigate the combined effects of trehalose and cations on the preservation of β-galactosidase in freeze-dried systems and their relationship to physical properties. Differential scanning calorimetry was employed to measure the glass transition temperature (T(g)) a...
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todo:paper_03044165_v1473_n2-3_p337_Mazzobre2023-10-03T15:20:41Z Combined effects of trehalose and cations on the thermal resistance of β-galactosidase in freeze-dried systems Mazzobre, M.F. Del Pilar Buera, M. β-Galactosidase Cation Crystallization Enzyme stabilization Freeze-drying Trehalose beta galactosidase cation cesium chloride magnesium chloride potassium chloride sodium chloride trehalose article crystallization differential scanning calorimetry enzyme stability freeze drying heat tolerance moisture priority journal X ray diffraction beta-Galactosidase Calorimetry, Differential Scanning Cesium Chlorides Crystallization Enzyme Stability Freeze Drying Heat Lactase Magnesium Chloride Potassium Chloride Sodium Chloride Trehalose The purpose of this study was to investigate the combined effects of trehalose and cations on the preservation of β-galactosidase in freeze-dried systems and their relationship to physical properties. Differential scanning calorimetry was employed to measure the glass transition temperature (T(g)) and the endothermal peak area, related to the amount of crystalline trehalose dihydrate present in the samples. In systems in which the trehalose matrix was humidified to conditions which allowed a high proportion of trehalose to crystallize, the enzyme was rapidly inactivated upon heating at 70°C. In these conditions the addition of CsCl, NaCl and particularly KCl or MgCl2, improved the enzyme stability with respect to that observed in matrices containing only trehalose. For a given moisture content, addition of salts produced very little change on the glass transition temperature; therefore the protective effect could not be attributed to a higher T(g) value. The crystallization of trehalose dihydrate in the humidified samples was delayed in the trehalose/salt systems (principally in the presence of Mg2+) and a parallel improvement of enzyme stability was observed. Copyright (C) 1999 Elsevier Science B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03044165_v1473_n2-3_p337_Mazzobre |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
β-Galactosidase Cation Crystallization Enzyme stabilization Freeze-drying Trehalose beta galactosidase cation cesium chloride magnesium chloride potassium chloride sodium chloride trehalose article crystallization differential scanning calorimetry enzyme stability freeze drying heat tolerance moisture priority journal X ray diffraction beta-Galactosidase Calorimetry, Differential Scanning Cesium Chlorides Crystallization Enzyme Stability Freeze Drying Heat Lactase Magnesium Chloride Potassium Chloride Sodium Chloride Trehalose |
| spellingShingle |
β-Galactosidase Cation Crystallization Enzyme stabilization Freeze-drying Trehalose beta galactosidase cation cesium chloride magnesium chloride potassium chloride sodium chloride trehalose article crystallization differential scanning calorimetry enzyme stability freeze drying heat tolerance moisture priority journal X ray diffraction beta-Galactosidase Calorimetry, Differential Scanning Cesium Chlorides Crystallization Enzyme Stability Freeze Drying Heat Lactase Magnesium Chloride Potassium Chloride Sodium Chloride Trehalose Mazzobre, M.F. Del Pilar Buera, M. Combined effects of trehalose and cations on the thermal resistance of β-galactosidase in freeze-dried systems |
| topic_facet |
β-Galactosidase Cation Crystallization Enzyme stabilization Freeze-drying Trehalose beta galactosidase cation cesium chloride magnesium chloride potassium chloride sodium chloride trehalose article crystallization differential scanning calorimetry enzyme stability freeze drying heat tolerance moisture priority journal X ray diffraction beta-Galactosidase Calorimetry, Differential Scanning Cesium Chlorides Crystallization Enzyme Stability Freeze Drying Heat Lactase Magnesium Chloride Potassium Chloride Sodium Chloride Trehalose |
| description |
The purpose of this study was to investigate the combined effects of trehalose and cations on the preservation of β-galactosidase in freeze-dried systems and their relationship to physical properties. Differential scanning calorimetry was employed to measure the glass transition temperature (T(g)) and the endothermal peak area, related to the amount of crystalline trehalose dihydrate present in the samples. In systems in which the trehalose matrix was humidified to conditions which allowed a high proportion of trehalose to crystallize, the enzyme was rapidly inactivated upon heating at 70°C. In these conditions the addition of CsCl, NaCl and particularly KCl or MgCl2, improved the enzyme stability with respect to that observed in matrices containing only trehalose. For a given moisture content, addition of salts produced very little change on the glass transition temperature; therefore the protective effect could not be attributed to a higher T(g) value. The crystallization of trehalose dihydrate in the humidified samples was delayed in the trehalose/salt systems (principally in the presence of Mg2+) and a parallel improvement of enzyme stability was observed. Copyright (C) 1999 Elsevier Science B.V. |
| format |
JOUR |
| author |
Mazzobre, M.F. Del Pilar Buera, M. |
| author_facet |
Mazzobre, M.F. Del Pilar Buera, M. |
| author_sort |
Mazzobre, M.F. |
| title |
Combined effects of trehalose and cations on the thermal resistance of β-galactosidase in freeze-dried systems |
| title_short |
Combined effects of trehalose and cations on the thermal resistance of β-galactosidase in freeze-dried systems |
| title_full |
Combined effects of trehalose and cations on the thermal resistance of β-galactosidase in freeze-dried systems |
| title_fullStr |
Combined effects of trehalose and cations on the thermal resistance of β-galactosidase in freeze-dried systems |
| title_full_unstemmed |
Combined effects of trehalose and cations on the thermal resistance of β-galactosidase in freeze-dried systems |
| title_sort |
combined effects of trehalose and cations on the thermal resistance of β-galactosidase in freeze-dried systems |
| url |
http://hdl.handle.net/20.500.12110/paper_03044165_v1473_n2-3_p337_Mazzobre |
| work_keys_str_mv |
AT mazzobremf combinedeffectsoftrehaloseandcationsonthethermalresistanceofbgalactosidaseinfreezedriedsystems AT delpilarbueram combinedeffectsoftrehaloseandcationsonthethermalresistanceofbgalactosidaseinfreezedriedsystems |
| _version_ |
1807319583783124992 |