Experimental cystic echinococcosis therapy: In vitro and in vivo combined 5-fluorouracil/albendazole treatment
Human cystic echinococcosis is a zoonosis caused by the larval stage of the tapeworm Echinococcus granulosus sensu lato (s. l.). Although benzimidazole compounds such as albendazole (ABZ) and mebendazole have been the cornerstone of chemotherapy for the disease, there is often no complete recovery a...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03044017_v245_n_p62_Pensel |
| Aporte de: |
| id |
todo:paper_03044017_v245_n_p62_Pensel |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_03044017_v245_n_p62_Pensel2023-10-03T15:20:36Z Experimental cystic echinococcosis therapy: In vitro and in vivo combined 5-fluorouracil/albendazole treatment Pensel, P.E. Elissondo, N. Gambino, G. Gamboa, G.U. Benoit, J.P. Elissondo, M.C. 5-fluorouracil Albendazole Cystic echinococcosis Echinococcus granulosus sensu stricto albendazole fluorouracil albendazole fluorouracil animal experiment animal model Article concentration response controlled study drug efficacy echinococcosis Echinococcus granulosus experimental infection female in vitro study in vivo study larval stage metacestode monotherapy mouse nonhuman parasite viability scanning electron microscopy suspension treatment response ultrastructure animal combination drug therapy dose response echinococcosis Albendazole Animals Dose-Response Relationship, Drug Drug Therapy, Combination Echinococcosis Echinococcus granulosus Female Fluorouracil Mice Human cystic echinococcosis is a zoonosis caused by the larval stage of the tapeworm Echinococcus granulosus sensu lato (s. l.). Although benzimidazole compounds such as albendazole (ABZ) and mebendazole have been the cornerstone of chemotherapy for the disease, there is often no complete recovery after treatment. Hence, new strategies are required to improve treatment of human cystic echinococcosis. The goals of the current study were as follows: (i) to evaluate the in vitro efficacy of the 5-fluorouracil (5-FU) and ABZ combination against E. granulosus s. l. protoscoleces and cysts, (ii) to compare the clinical efficacy of 5-FU alone or in combination with ABZ in infected mice. The combination of 5-FU + ABZ had a stronger in vitro effect against larval stage than that did both drugs alone. Even at the lowest concentration of 5-FU + ABZ combination (1 μg/ml), the reduction of the viability of protoscoleces and cysts was greater than that observed with drugs alone at 10 μg/ml. The results were confirmed at the ultrastructural level by scanning electron microscopy. These data helped to justify the in vivo investigations assessing the therapeutic potential of the combination of 5-FU and ABZ suspension in CF-1 mice infected with E. granulosus sensu stricto (s. s.) metacestodes. Treatment with 5-FU (10 mg/kg) or 5-FU (10 mg/kg) + ABZ suspension (5 mg/kg) reduced the weight of cysts recovered from mice compared with control groups. Interestingly, the effect of 5-FU given weekly for 5 consecutive weeks was comparable to that observed with ABZ suspension under a daily schedule during 30 days. Co-administration of 5-FU with ABZ did not enhance the in vivo efficacy of drugs alone calculated in relation to cysts weights. However, the combination provoked greater ultrastructural alterations compared to the monotherapy. In conclusion, we demonstrated the efficacy of 5-FU either alone or co-administrated with ABZ against murine experimental cystic echinococcosis. Since 5-FU treatments did not cause toxic effect in mice, further in vivo studies will be performed by adjusting the dosage and the frequency of treatments. © 2017 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03044017_v245_n_p62_Pensel |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
5-fluorouracil Albendazole Cystic echinococcosis Echinococcus granulosus sensu stricto albendazole fluorouracil albendazole fluorouracil animal experiment animal model Article concentration response controlled study drug efficacy echinococcosis Echinococcus granulosus experimental infection female in vitro study in vivo study larval stage metacestode monotherapy mouse nonhuman parasite viability scanning electron microscopy suspension treatment response ultrastructure animal combination drug therapy dose response echinococcosis Albendazole Animals Dose-Response Relationship, Drug Drug Therapy, Combination Echinococcosis Echinococcus granulosus Female Fluorouracil Mice |
| spellingShingle |
5-fluorouracil Albendazole Cystic echinococcosis Echinococcus granulosus sensu stricto albendazole fluorouracil albendazole fluorouracil animal experiment animal model Article concentration response controlled study drug efficacy echinococcosis Echinococcus granulosus experimental infection female in vitro study in vivo study larval stage metacestode monotherapy mouse nonhuman parasite viability scanning electron microscopy suspension treatment response ultrastructure animal combination drug therapy dose response echinococcosis Albendazole Animals Dose-Response Relationship, Drug Drug Therapy, Combination Echinococcosis Echinococcus granulosus Female Fluorouracil Mice Pensel, P.E. Elissondo, N. Gambino, G. Gamboa, G.U. Benoit, J.P. Elissondo, M.C. Experimental cystic echinococcosis therapy: In vitro and in vivo combined 5-fluorouracil/albendazole treatment |
| topic_facet |
5-fluorouracil Albendazole Cystic echinococcosis Echinococcus granulosus sensu stricto albendazole fluorouracil albendazole fluorouracil animal experiment animal model Article concentration response controlled study drug efficacy echinococcosis Echinococcus granulosus experimental infection female in vitro study in vivo study larval stage metacestode monotherapy mouse nonhuman parasite viability scanning electron microscopy suspension treatment response ultrastructure animal combination drug therapy dose response echinococcosis Albendazole Animals Dose-Response Relationship, Drug Drug Therapy, Combination Echinococcosis Echinococcus granulosus Female Fluorouracil Mice |
| description |
Human cystic echinococcosis is a zoonosis caused by the larval stage of the tapeworm Echinococcus granulosus sensu lato (s. l.). Although benzimidazole compounds such as albendazole (ABZ) and mebendazole have been the cornerstone of chemotherapy for the disease, there is often no complete recovery after treatment. Hence, new strategies are required to improve treatment of human cystic echinococcosis. The goals of the current study were as follows: (i) to evaluate the in vitro efficacy of the 5-fluorouracil (5-FU) and ABZ combination against E. granulosus s. l. protoscoleces and cysts, (ii) to compare the clinical efficacy of 5-FU alone or in combination with ABZ in infected mice. The combination of 5-FU + ABZ had a stronger in vitro effect against larval stage than that did both drugs alone. Even at the lowest concentration of 5-FU + ABZ combination (1 μg/ml), the reduction of the viability of protoscoleces and cysts was greater than that observed with drugs alone at 10 μg/ml. The results were confirmed at the ultrastructural level by scanning electron microscopy. These data helped to justify the in vivo investigations assessing the therapeutic potential of the combination of 5-FU and ABZ suspension in CF-1 mice infected with E. granulosus sensu stricto (s. s.) metacestodes. Treatment with 5-FU (10 mg/kg) or 5-FU (10 mg/kg) + ABZ suspension (5 mg/kg) reduced the weight of cysts recovered from mice compared with control groups. Interestingly, the effect of 5-FU given weekly for 5 consecutive weeks was comparable to that observed with ABZ suspension under a daily schedule during 30 days. Co-administration of 5-FU with ABZ did not enhance the in vivo efficacy of drugs alone calculated in relation to cysts weights. However, the combination provoked greater ultrastructural alterations compared to the monotherapy. In conclusion, we demonstrated the efficacy of 5-FU either alone or co-administrated with ABZ against murine experimental cystic echinococcosis. Since 5-FU treatments did not cause toxic effect in mice, further in vivo studies will be performed by adjusting the dosage and the frequency of treatments. © 2017 Elsevier B.V. |
| format |
JOUR |
| author |
Pensel, P.E. Elissondo, N. Gambino, G. Gamboa, G.U. Benoit, J.P. Elissondo, M.C. |
| author_facet |
Pensel, P.E. Elissondo, N. Gambino, G. Gamboa, G.U. Benoit, J.P. Elissondo, M.C. |
| author_sort |
Pensel, P.E. |
| title |
Experimental cystic echinococcosis therapy: In vitro and in vivo combined 5-fluorouracil/albendazole treatment |
| title_short |
Experimental cystic echinococcosis therapy: In vitro and in vivo combined 5-fluorouracil/albendazole treatment |
| title_full |
Experimental cystic echinococcosis therapy: In vitro and in vivo combined 5-fluorouracil/albendazole treatment |
| title_fullStr |
Experimental cystic echinococcosis therapy: In vitro and in vivo combined 5-fluorouracil/albendazole treatment |
| title_full_unstemmed |
Experimental cystic echinococcosis therapy: In vitro and in vivo combined 5-fluorouracil/albendazole treatment |
| title_sort |
experimental cystic echinococcosis therapy: in vitro and in vivo combined 5-fluorouracil/albendazole treatment |
| url |
http://hdl.handle.net/20.500.12110/paper_03044017_v245_n_p62_Pensel |
| work_keys_str_mv |
AT penselpe experimentalcysticechinococcosistherapyinvitroandinvivocombined5fluorouracilalbendazoletreatment AT elissondon experimentalcysticechinococcosistherapyinvitroandinvivocombined5fluorouracilalbendazoletreatment AT gambinog experimentalcysticechinococcosistherapyinvitroandinvivocombined5fluorouracilalbendazoletreatment AT gamboagu experimentalcysticechinococcosistherapyinvitroandinvivocombined5fluorouracilalbendazoletreatment AT benoitjp experimentalcysticechinococcosistherapyinvitroandinvivocombined5fluorouracilalbendazoletreatment AT elissondomc experimentalcysticechinococcosistherapyinvitroandinvivocombined5fluorouracilalbendazoletreatment |
| _version_ |
1807322938681065472 |