Molecular evidence for a novel encapsulated genotype of Trichinella from Patagonia, Argentina

At present, Trichinella spiralis is the only species of this genus reported from South America. Herein, we detail a molecular analysis of a new encapsulated isolate of muscle larvae of Trichinella, found in a mountain lion (Puma concolor) coming from the Patagonia, Argentina. We studied three DNA re...

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Autores principales:	Krivokapich, S.J., Prous, C.L.G., Gatti, G.M., Confalonieri, V., Molina, V., Matarasso, H., Guarnera, E.
Formato:	JOUR
Materias:	Patagonia Phylogeny Puma concolor Trichinella cytochrome c oxidase DNA 5S Argentina article controlled study DNA sequence female genetic difference genotype lion nonhuman nucleotide sequence phylogeny swine Trichinella spiralis Animals Genotype Puma Trichinosis
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03044017_v156_n3-4_p234_Krivokapich
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_03044017_v156_n3-4_p234_Krivokapich2023-10-03T15:20:33Z Molecular evidence for a novel encapsulated genotype of Trichinella from Patagonia, Argentina Krivokapich, S.J. Prous, C.L.G. Gatti, G.M. Confalonieri, V. Molina, V. Matarasso, H. Guarnera, E. Patagonia Phylogeny Puma concolor Trichinella cytochrome c oxidase DNA 5S Argentina article controlled study DNA sequence female genetic difference genotype lion nonhuman nucleotide sequence phylogeny swine Trichinella spiralis Animals Argentina Genotype Phylogeny Puma Trichinella Trichinosis Puma concolor Trichinella Trichinella spiralis At present, Trichinella spiralis is the only species of this genus reported from South America. Herein, we detail a molecular analysis of a new encapsulated isolate of muscle larvae of Trichinella, found in a mountain lion (Puma concolor) coming from the Patagonia, Argentina. We studied three DNA regions previously probed to be useful for the identification of all eleven recognized Trichinella genotypes: expansion segment 5 (ES5), cytochrome c-oxidase subunit I (COI) and 5S ribosomal DNA intergenic spacer region (5S ISR). BLAST searches with these DNA sequences showed that the mitochondrial and nuclear ribosomal regions most closely resemble other Trichinella sequences available in GenBank. However, they did not exactly match any of the eleven recognized genotypes. The phylogenetic analysis from COI and 5S ISR sequences showed that the mountain lion isolate is grouped with encapsulated members, in concordance with morphological data. Furthermore, this new isolate was located at the base of the encapsulated genotypes, signifying that it is an old genotype that could have emerged earliest in this group. These data strongly suggest that this isolate from the Patagonia represents the twelfth genotype (T12) described in the genus Trichinella. Nevertheless, further studies are necessary to adequately establish this isolate as a unique genotype. © 2008 Elsevier B.V. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03044017_v156_n3-4_p234_Krivokapich
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Patagonia Phylogeny Puma concolor Trichinella cytochrome c oxidase DNA 5S Argentina article controlled study DNA sequence female genetic difference genotype lion nonhuman nucleotide sequence phylogeny swine Trichinella spiralis Animals Argentina Genotype Phylogeny Puma Trichinella Trichinosis Puma concolor Trichinella Trichinella spiralis
spellingShingle	Patagonia Phylogeny Puma concolor Trichinella cytochrome c oxidase DNA 5S Argentina article controlled study DNA sequence female genetic difference genotype lion nonhuman nucleotide sequence phylogeny swine Trichinella spiralis Animals Argentina Genotype Phylogeny Puma Trichinella Trichinosis Puma concolor Trichinella Trichinella spiralis Krivokapich, S.J. Prous, C.L.G. Gatti, G.M. Confalonieri, V. Molina, V. Matarasso, H. Guarnera, E. Molecular evidence for a novel encapsulated genotype of Trichinella from Patagonia, Argentina
topic_facet	Patagonia Phylogeny Puma concolor Trichinella cytochrome c oxidase DNA 5S Argentina article controlled study DNA sequence female genetic difference genotype lion nonhuman nucleotide sequence phylogeny swine Trichinella spiralis Animals Argentina Genotype Phylogeny Puma Trichinella Trichinosis Puma concolor Trichinella Trichinella spiralis
description	At present, Trichinella spiralis is the only species of this genus reported from South America. Herein, we detail a molecular analysis of a new encapsulated isolate of muscle larvae of Trichinella, found in a mountain lion (Puma concolor) coming from the Patagonia, Argentina. We studied three DNA regions previously probed to be useful for the identification of all eleven recognized Trichinella genotypes: expansion segment 5 (ES5), cytochrome c-oxidase subunit I (COI) and 5S ribosomal DNA intergenic spacer region (5S ISR). BLAST searches with these DNA sequences showed that the mitochondrial and nuclear ribosomal regions most closely resemble other Trichinella sequences available in GenBank. However, they did not exactly match any of the eleven recognized genotypes. The phylogenetic analysis from COI and 5S ISR sequences showed that the mountain lion isolate is grouped with encapsulated members, in concordance with morphological data. Furthermore, this new isolate was located at the base of the encapsulated genotypes, signifying that it is an old genotype that could have emerged earliest in this group. These data strongly suggest that this isolate from the Patagonia represents the twelfth genotype (T12) described in the genus Trichinella. Nevertheless, further studies are necessary to adequately establish this isolate as a unique genotype. © 2008 Elsevier B.V. All rights reserved.
format	JOUR
author	Krivokapich, S.J. Prous, C.L.G. Gatti, G.M. Confalonieri, V. Molina, V. Matarasso, H. Guarnera, E.
author_facet	Krivokapich, S.J. Prous, C.L.G. Gatti, G.M. Confalonieri, V. Molina, V. Matarasso, H. Guarnera, E.
author_sort	Krivokapich, S.J.
title	Molecular evidence for a novel encapsulated genotype of Trichinella from Patagonia, Argentina
title_short	Molecular evidence for a novel encapsulated genotype of Trichinella from Patagonia, Argentina
title_full	Molecular evidence for a novel encapsulated genotype of Trichinella from Patagonia, Argentina
title_fullStr	Molecular evidence for a novel encapsulated genotype of Trichinella from Patagonia, Argentina
title_full_unstemmed	Molecular evidence for a novel encapsulated genotype of Trichinella from Patagonia, Argentina
title_sort	molecular evidence for a novel encapsulated genotype of trichinella from patagonia, argentina
url	http://hdl.handle.net/20.500.12110/paper_03044017_v156_n3-4_p234_Krivokapich
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Molecular evidence for a novel encapsulated genotype of Trichinella from Patagonia, Argentina

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