Alterations in the prolactin secretion in streptozotocin-induced diabetic rats. Correlation with pituitary and hypothalamus estradiol receptors
The present study examined the effects of streptozotocin-induced diabetes on prolactin (Prl) secretion and its correlation with estrogen receptor levels in the anterior pituitary and hypothalamus. Prl was measured in adult ovariectomized rats and after treatment (10 μg estradiol benzoate (Eb) 48.24...
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todo:paper_03037207_v43_n2-3_p135_Tesone2023-10-03T15:19:58Z Alterations in the prolactin secretion in streptozotocin-induced diabetic rats. Correlation with pituitary and hypothalamus estradiol receptors Tesone, M. Ladenheim, R.G. Charreau, E.H. diabetic rats estradiol receptors prolactin estradiol benzoate estradiol receptor insulin protirelin radioisotope streptozocin animal cell animal experiment animal model central nervous system drug mechanism drug receptor binding drug sensitivity endocrine system estradiol h 3 etiology female genital system hypophysis hypothalamus intravenous drug administration nonhuman ovariectomy pancreas pharmacokinetics priority journal prolactin release rat reproduction streptozocin diabetes subcutaneous drug administration therapy Animal Diabetes Mellitus, Experimental Estradiol Female Hypothalamus Insulin Ovariectomy Pituitary Gland Prolactin Protirelin Rats Rats, Inbred Strains Receptors, Estradiol Receptors, Estrogen Support, Non-U.S. Gov't The present study examined the effects of streptozotocin-induced diabetes on prolactin (Prl) secretion and its correlation with estrogen receptor levels in the anterior pituitary and hypothalamus. Prl was measured in adult ovariectomized rats and after treatment (10 μg estradiol benzoate (Eb) 48.24 and 1 h before experiments) or acute TRH administration (4 μg/kg body weight). Substantial decreases in estradiol- and TRH-induced Prl release were observed in diabetic rats. Insulin therapy was able to restore this response. Measurement of nuclear estradiol receptors by exchange assay in the pituitary of Eb-treated rats revealed a significant reduction in receptor levels in the diabetic group and a restoration to normal values in insulin-treated diabetic rats. Similar results were obtained by measuring total pituitary receptor content (cytosolic plus nuclear receptor). No significant changes were observed observed in nuclear hypothalamic estradiol receptors. However, the number of total hypothalamic estradiol receptors was diminished in diabetic rats although the translocation was proportionally greater in these animals. These results indicate that the disrupted reproductive functions described in streptozotocin diabetic rats may be due, at least in part, to deficiencies in Prl secretions and pituitary estradiol action. © 1985. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ladenheim, R.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03037207_v43_n2-3_p135_Tesone |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
diabetic rats estradiol receptors prolactin estradiol benzoate estradiol receptor insulin protirelin radioisotope streptozocin animal cell animal experiment animal model central nervous system drug mechanism drug receptor binding drug sensitivity endocrine system estradiol h 3 etiology female genital system hypophysis hypothalamus intravenous drug administration nonhuman ovariectomy pancreas pharmacokinetics priority journal prolactin release rat reproduction streptozocin diabetes subcutaneous drug administration therapy Animal Diabetes Mellitus, Experimental Estradiol Female Hypothalamus Insulin Ovariectomy Pituitary Gland Prolactin Protirelin Rats Rats, Inbred Strains Receptors, Estradiol Receptors, Estrogen Support, Non-U.S. Gov't |
| spellingShingle |
diabetic rats estradiol receptors prolactin estradiol benzoate estradiol receptor insulin protirelin radioisotope streptozocin animal cell animal experiment animal model central nervous system drug mechanism drug receptor binding drug sensitivity endocrine system estradiol h 3 etiology female genital system hypophysis hypothalamus intravenous drug administration nonhuman ovariectomy pancreas pharmacokinetics priority journal prolactin release rat reproduction streptozocin diabetes subcutaneous drug administration therapy Animal Diabetes Mellitus, Experimental Estradiol Female Hypothalamus Insulin Ovariectomy Pituitary Gland Prolactin Protirelin Rats Rats, Inbred Strains Receptors, Estradiol Receptors, Estrogen Support, Non-U.S. Gov't Tesone, M. Ladenheim, R.G. Charreau, E.H. Alterations in the prolactin secretion in streptozotocin-induced diabetic rats. Correlation with pituitary and hypothalamus estradiol receptors |
| topic_facet |
diabetic rats estradiol receptors prolactin estradiol benzoate estradiol receptor insulin protirelin radioisotope streptozocin animal cell animal experiment animal model central nervous system drug mechanism drug receptor binding drug sensitivity endocrine system estradiol h 3 etiology female genital system hypophysis hypothalamus intravenous drug administration nonhuman ovariectomy pancreas pharmacokinetics priority journal prolactin release rat reproduction streptozocin diabetes subcutaneous drug administration therapy Animal Diabetes Mellitus, Experimental Estradiol Female Hypothalamus Insulin Ovariectomy Pituitary Gland Prolactin Protirelin Rats Rats, Inbred Strains Receptors, Estradiol Receptors, Estrogen Support, Non-U.S. Gov't |
| description |
The present study examined the effects of streptozotocin-induced diabetes on prolactin (Prl) secretion and its correlation with estrogen receptor levels in the anterior pituitary and hypothalamus. Prl was measured in adult ovariectomized rats and after treatment (10 μg estradiol benzoate (Eb) 48.24 and 1 h before experiments) or acute TRH administration (4 μg/kg body weight). Substantial decreases in estradiol- and TRH-induced Prl release were observed in diabetic rats. Insulin therapy was able to restore this response. Measurement of nuclear estradiol receptors by exchange assay in the pituitary of Eb-treated rats revealed a significant reduction in receptor levels in the diabetic group and a restoration to normal values in insulin-treated diabetic rats. Similar results were obtained by measuring total pituitary receptor content (cytosolic plus nuclear receptor). No significant changes were observed observed in nuclear hypothalamic estradiol receptors. However, the number of total hypothalamic estradiol receptors was diminished in diabetic rats although the translocation was proportionally greater in these animals. These results indicate that the disrupted reproductive functions described in streptozotocin diabetic rats may be due, at least in part, to deficiencies in Prl secretions and pituitary estradiol action. © 1985. |
| format |
JOUR |
| author |
Tesone, M. Ladenheim, R.G. Charreau, E.H. |
| author_facet |
Tesone, M. Ladenheim, R.G. Charreau, E.H. |
| author_sort |
Tesone, M. |
| title |
Alterations in the prolactin secretion in streptozotocin-induced diabetic rats. Correlation with pituitary and hypothalamus estradiol receptors |
| title_short |
Alterations in the prolactin secretion in streptozotocin-induced diabetic rats. Correlation with pituitary and hypothalamus estradiol receptors |
| title_full |
Alterations in the prolactin secretion in streptozotocin-induced diabetic rats. Correlation with pituitary and hypothalamus estradiol receptors |
| title_fullStr |
Alterations in the prolactin secretion in streptozotocin-induced diabetic rats. Correlation with pituitary and hypothalamus estradiol receptors |
| title_full_unstemmed |
Alterations in the prolactin secretion in streptozotocin-induced diabetic rats. Correlation with pituitary and hypothalamus estradiol receptors |
| title_sort |
alterations in the prolactin secretion in streptozotocin-induced diabetic rats. correlation with pituitary and hypothalamus estradiol receptors |
| url |
http://hdl.handle.net/20.500.12110/paper_03037207_v43_n2-3_p135_Tesone |
| work_keys_str_mv |
AT tesonem alterationsintheprolactinsecretioninstreptozotocininduceddiabeticratscorrelationwithpituitaryandhypothalamusestradiolreceptors AT ladenheimrg alterationsintheprolactinsecretioninstreptozotocininduceddiabeticratscorrelationwithpituitaryandhypothalamusestradiolreceptors AT charreaueh alterationsintheprolactinsecretioninstreptozotocininduceddiabeticratscorrelationwithpituitaryandhypothalamusestradiolreceptors |
| _version_ |
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