Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells

Epidermal growth factor (EGF) has been suggested to play a key role in the maintenance of epithelial cell survival during lactation. Previously, we demonstrated that EGF dependent activation of PI3K pathway prevents apoptosis in confluent murine HC11 cells cultured under low nutrient conditions. The...

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Autores principales:	Grinman, D.Y., Romorini, L., Presman, D.M., Rocha-Viegas, L., Coso, O.A., Davio, C., Pecci, A.
Formato:	JOUR
Materias:	Apoptosis Bcl-X CAMP CREB EGF Mammary epithelial cells 3' 5' cyclic adenosine monophosphate adenosine phosphate adenylate cyclase cyclic AMP responsive element binding protein epidermal growth factor phosphatidylinositol 3 kinase protein bcl x protein bcl xl protein kinase B unclassified drug Bcl2l1 protein, mouse Creb1 protein, mouse culture medium cyclic AMP animal cell apoptosis Article breast epithelium cell cell death cell survival cell viability controlled study correlational study enzyme activation mouse nonhuman priority journal promoter region protein expression protein function protein protein interaction signal transduction upregulation animal cell culture technique cell line chemistry cytology drug effects female genetics metabolism pharmacology udder Animals bcl-X Protein Cell Culture Techniques Cell Line Cell Survival Culture Media Cyclic AMP Cyclic AMP Response Element-Binding Protein Epidermal Growth Factor Female Mammary Glands, Animal Mice Promoter Regions, Genetic Signal Transduction
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03037207_v419_n_p259_Grinman
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_03037207_v419_n_p259_Grinman2023-10-03T15:19:58Z Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells Grinman, D.Y. Romorini, L. Presman, D.M. Rocha-Viegas, L. Coso, O.A. Davio, C. Pecci, A. Apoptosis Bcl-X CAMP CREB EGF Mammary epithelial cells 3' 5' cyclic adenosine monophosphate adenosine phosphate adenylate cyclase cyclic AMP responsive element binding protein epidermal growth factor phosphatidylinositol 3 kinase protein bcl x protein bcl xl protein kinase B unclassified drug Bcl2l1 protein, mouse Creb1 protein, mouse culture medium cyclic AMP cyclic AMP responsive element binding protein epidermal growth factor protein bcl x animal cell apoptosis Article breast epithelium cell cell death cell survival cell viability controlled study correlational study enzyme activation mouse nonhuman priority journal promoter region protein expression protein function protein protein interaction signal transduction upregulation animal cell culture technique cell line cell survival chemistry culture medium cytology drug effects female genetics metabolism pharmacology udder Animals bcl-X Protein Cell Culture Techniques Cell Line Cell Survival Culture Media Cyclic AMP Cyclic AMP Response Element-Binding Protein Epidermal Growth Factor Female Mammary Glands, Animal Mice Promoter Regions, Genetic Signal Transduction Epidermal growth factor (EGF) has been suggested to play a key role in the maintenance of epithelial cell survival during lactation. Previously, we demonstrated that EGF dependent activation of PI3K pathway prevents apoptosis in confluent murine HC11 cells cultured under low nutrient conditions. The EGF protective effect is associated with increased levels of the antiapoptotic protein Bcl-XL. Here, we identify the EGF-dependent mechanism involved in cell survival that converges in the regulation of bcl-X expression by activated CREB. EGF induces Bcl-XL expression through activation of a unique bcl-X promoter, the P1; being not only the PI3K/AKT signaling pathway but also the increase in cAMP levels and the concomitant PKA/CREB activation necessary for both bcl-XL upregulation and apoptosis avoidance. Results presented in this work suggest the existence of a novel connection between the EGF receptor and the adenylate cyclase that would have an impact in preventing apoptosis under low nutrient conditions. © 2015 Elsevier Ireland Ltd. Fil:Grinman, D.Y. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romorini, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Presman, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rocha-Viegas, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pecci, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03037207_v419_n_p259_Grinman
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Apoptosis Bcl-X CAMP CREB EGF Mammary epithelial cells 3' 5' cyclic adenosine monophosphate adenosine phosphate adenylate cyclase cyclic AMP responsive element binding protein epidermal growth factor phosphatidylinositol 3 kinase protein bcl x protein bcl xl protein kinase B unclassified drug Bcl2l1 protein, mouse Creb1 protein, mouse culture medium cyclic AMP cyclic AMP responsive element binding protein epidermal growth factor protein bcl x animal cell apoptosis Article breast epithelium cell cell death cell survival cell viability controlled study correlational study enzyme activation mouse nonhuman priority journal promoter region protein expression protein function protein protein interaction signal transduction upregulation animal cell culture technique cell line cell survival chemistry culture medium cytology drug effects female genetics metabolism pharmacology udder Animals bcl-X Protein Cell Culture Techniques Cell Line Cell Survival Culture Media Cyclic AMP Cyclic AMP Response Element-Binding Protein Epidermal Growth Factor Female Mammary Glands, Animal Mice Promoter Regions, Genetic Signal Transduction
spellingShingle	Apoptosis Bcl-X CAMP CREB EGF Mammary epithelial cells 3' 5' cyclic adenosine monophosphate adenosine phosphate adenylate cyclase cyclic AMP responsive element binding protein epidermal growth factor phosphatidylinositol 3 kinase protein bcl x protein bcl xl protein kinase B unclassified drug Bcl2l1 protein, mouse Creb1 protein, mouse culture medium cyclic AMP cyclic AMP responsive element binding protein epidermal growth factor protein bcl x animal cell apoptosis Article breast epithelium cell cell death cell survival cell viability controlled study correlational study enzyme activation mouse nonhuman priority journal promoter region protein expression protein function protein protein interaction signal transduction upregulation animal cell culture technique cell line cell survival chemistry culture medium cytology drug effects female genetics metabolism pharmacology udder Animals bcl-X Protein Cell Culture Techniques Cell Line Cell Survival Culture Media Cyclic AMP Cyclic AMP Response Element-Binding Protein Epidermal Growth Factor Female Mammary Glands, Animal Mice Promoter Regions, Genetic Signal Transduction Grinman, D.Y. Romorini, L. Presman, D.M. Rocha-Viegas, L. Coso, O.A. Davio, C. Pecci, A. Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells
topic_facet	Apoptosis Bcl-X CAMP CREB EGF Mammary epithelial cells 3' 5' cyclic adenosine monophosphate adenosine phosphate adenylate cyclase cyclic AMP responsive element binding protein epidermal growth factor phosphatidylinositol 3 kinase protein bcl x protein bcl xl protein kinase B unclassified drug Bcl2l1 protein, mouse Creb1 protein, mouse culture medium cyclic AMP cyclic AMP responsive element binding protein epidermal growth factor protein bcl x animal cell apoptosis Article breast epithelium cell cell death cell survival cell viability controlled study correlational study enzyme activation mouse nonhuman priority journal promoter region protein expression protein function protein protein interaction signal transduction upregulation animal cell culture technique cell line cell survival chemistry culture medium cytology drug effects female genetics metabolism pharmacology udder Animals bcl-X Protein Cell Culture Techniques Cell Line Cell Survival Culture Media Cyclic AMP Cyclic AMP Response Element-Binding Protein Epidermal Growth Factor Female Mammary Glands, Animal Mice Promoter Regions, Genetic Signal Transduction
description	Epidermal growth factor (EGF) has been suggested to play a key role in the maintenance of epithelial cell survival during lactation. Previously, we demonstrated that EGF dependent activation of PI3K pathway prevents apoptosis in confluent murine HC11 cells cultured under low nutrient conditions. The EGF protective effect is associated with increased levels of the antiapoptotic protein Bcl-XL. Here, we identify the EGF-dependent mechanism involved in cell survival that converges in the regulation of bcl-X expression by activated CREB. EGF induces Bcl-XL expression through activation of a unique bcl-X promoter, the P1; being not only the PI3K/AKT signaling pathway but also the increase in cAMP levels and the concomitant PKA/CREB activation necessary for both bcl-XL upregulation and apoptosis avoidance. Results presented in this work suggest the existence of a novel connection between the EGF receptor and the adenylate cyclase that would have an impact in preventing apoptosis under low nutrient conditions. © 2015 Elsevier Ireland Ltd.
format	JOUR
author	Grinman, D.Y. Romorini, L. Presman, D.M. Rocha-Viegas, L. Coso, O.A. Davio, C. Pecci, A.
author_facet	Grinman, D.Y. Romorini, L. Presman, D.M. Rocha-Viegas, L. Coso, O.A. Davio, C. Pecci, A.
author_sort	Grinman, D.Y.
title	Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells
title_short	Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells
title_full	Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells
title_fullStr	Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells
title_full_unstemmed	Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells
title_sort	role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells
url	http://hdl.handle.net/20.500.12110/paper_03037207_v419_n_p259_Grinman
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Role of 3'-5'-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells

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