Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice
Neonatal androgenization masculinizes the GH axis and thus may impact on liver gene regulation. Neonatal testosterone administration to female mice decreased (defeminized) female predominant GH-dependent liver gene expression (Hnf6, Adh1, Prlr, Cyp3a41) and did not modify male predominant genes (Cyp...
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todo:paper_03037207_v382_n2_p825_Ramirez2023-10-03T15:19:56Z Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice Ramirez, M.C. Zubeldía-Brenner, L. Wargon, V. Ornstein, A.M. Becu-Villalobos, D. Cyps DNA methylation GH Liver Neonatal testosterone Sexual differences androgen DNA endocrine disruptor growth hormone messenger RNA testosterone animal experiment animal tissue article Cyp7b1 gene DNA methylation female gene expression Hnf6 gene liver male mouse newborn nonhuman nucleotide sequence priority journal promoter region sex difference sex differentiation sexual development signal transduction Cyps DNA methylation GH Liver Neonatal testosterone Sexual differences Androgens Animals Animals, Newborn DNA Methylation Female Gene Expression Regulation, Developmental Growth Hormone Hepatocyte Nuclear Factor 6 Liver Male Mice Promoter Regions, Genetic Sex Characteristics Signal Transduction Steroid Hydroxylases Testosterone Virilism Neonatal androgenization masculinizes the GH axis and thus may impact on liver gene regulation. Neonatal testosterone administration to female mice decreased (defeminized) female predominant GH-dependent liver gene expression (Hnf6, Adh1, Prlr, Cyp3a41) and did not modify male predominant genes (Cyp7b1, Cyp4a12, Slp). Female predominance of Cis mRNA, an inhibitor of episodic GH signaling pathway, was unaltered. At birth, Cyp7b1 promoter exhibited a higher methylation status in female livers, while the Hnf6 promoter was equally methylated in both sexes; no differences in gene expression were detected at this age. In adulthood, consistent with sex specific predominance, lower methylation status was determined for the Cyp7b1 promoter in males, and for the Hnf6 promoter in females, and this last difference was prevented by neonatal androgenization. Therefore, early steroid treatment or eventually endocrine disruptor exposure may alter methylation status and sexual dimorphic expression of liver genes, and consequently modify liver physiology in females. © 2013 Elsevier Ireland Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03037207_v382_n2_p825_Ramirez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cyps DNA methylation GH Liver Neonatal testosterone Sexual differences androgen DNA endocrine disruptor growth hormone messenger RNA testosterone animal experiment animal tissue article Cyp7b1 gene DNA methylation female gene expression Hnf6 gene liver male mouse newborn nonhuman nucleotide sequence priority journal promoter region sex difference sex differentiation sexual development signal transduction Cyps DNA methylation GH Liver Neonatal testosterone Sexual differences Androgens Animals Animals, Newborn DNA Methylation Female Gene Expression Regulation, Developmental Growth Hormone Hepatocyte Nuclear Factor 6 Liver Male Mice Promoter Regions, Genetic Sex Characteristics Signal Transduction Steroid Hydroxylases Testosterone Virilism |
| spellingShingle |
Cyps DNA methylation GH Liver Neonatal testosterone Sexual differences androgen DNA endocrine disruptor growth hormone messenger RNA testosterone animal experiment animal tissue article Cyp7b1 gene DNA methylation female gene expression Hnf6 gene liver male mouse newborn nonhuman nucleotide sequence priority journal promoter region sex difference sex differentiation sexual development signal transduction Cyps DNA methylation GH Liver Neonatal testosterone Sexual differences Androgens Animals Animals, Newborn DNA Methylation Female Gene Expression Regulation, Developmental Growth Hormone Hepatocyte Nuclear Factor 6 Liver Male Mice Promoter Regions, Genetic Sex Characteristics Signal Transduction Steroid Hydroxylases Testosterone Virilism Ramirez, M.C. Zubeldía-Brenner, L. Wargon, V. Ornstein, A.M. Becu-Villalobos, D. Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice |
| topic_facet |
Cyps DNA methylation GH Liver Neonatal testosterone Sexual differences androgen DNA endocrine disruptor growth hormone messenger RNA testosterone animal experiment animal tissue article Cyp7b1 gene DNA methylation female gene expression Hnf6 gene liver male mouse newborn nonhuman nucleotide sequence priority journal promoter region sex difference sex differentiation sexual development signal transduction Cyps DNA methylation GH Liver Neonatal testosterone Sexual differences Androgens Animals Animals, Newborn DNA Methylation Female Gene Expression Regulation, Developmental Growth Hormone Hepatocyte Nuclear Factor 6 Liver Male Mice Promoter Regions, Genetic Sex Characteristics Signal Transduction Steroid Hydroxylases Testosterone Virilism |
| description |
Neonatal androgenization masculinizes the GH axis and thus may impact on liver gene regulation. Neonatal testosterone administration to female mice decreased (defeminized) female predominant GH-dependent liver gene expression (Hnf6, Adh1, Prlr, Cyp3a41) and did not modify male predominant genes (Cyp7b1, Cyp4a12, Slp). Female predominance of Cis mRNA, an inhibitor of episodic GH signaling pathway, was unaltered. At birth, Cyp7b1 promoter exhibited a higher methylation status in female livers, while the Hnf6 promoter was equally methylated in both sexes; no differences in gene expression were detected at this age. In adulthood, consistent with sex specific predominance, lower methylation status was determined for the Cyp7b1 promoter in males, and for the Hnf6 promoter in females, and this last difference was prevented by neonatal androgenization. Therefore, early steroid treatment or eventually endocrine disruptor exposure may alter methylation status and sexual dimorphic expression of liver genes, and consequently modify liver physiology in females. © 2013 Elsevier Ireland Ltd. |
| format |
JOUR |
| author |
Ramirez, M.C. Zubeldía-Brenner, L. Wargon, V. Ornstein, A.M. Becu-Villalobos, D. |
| author_facet |
Ramirez, M.C. Zubeldía-Brenner, L. Wargon, V. Ornstein, A.M. Becu-Villalobos, D. |
| author_sort |
Ramirez, M.C. |
| title |
Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice |
| title_short |
Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice |
| title_full |
Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice |
| title_fullStr |
Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice |
| title_full_unstemmed |
Expression and methylation status of female-predominant GH-dependent liver genes are modified by neonatal androgenization in female mice |
| title_sort |
expression and methylation status of female-predominant gh-dependent liver genes are modified by neonatal androgenization in female mice |
| url |
http://hdl.handle.net/20.500.12110/paper_03037207_v382_n2_p825_Ramirez |
| work_keys_str_mv |
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1782030705960681472 |