Activin and follistatin in rat mammary gland
Mammary gland morphogenesis and differentiation are mediated through the combined activities of systemic hormones and locally synthesized growth factors. Activin, a member of the transforming growth factor (TGF)-β superfamily, is known to regulate the growth and differentiation of several cell types...
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todo:paper_03037207_v221_n1-2_p9_Bussmann2023-10-03T15:19:48Z Activin and follistatin in rat mammary gland Bussmann, U.A. Lanuza, G.M. Bussmann, L.E. Differentiation Growth Growth factors Lactogenesis Pregnancy activin activin A activin receptor activin receptor 2a activin receptor 2b follistatin ligand messenger RNA myelopid unclassified drug acinar cell animal experiment article cell differentiation cell isolation cell proliferation controlled study female growth regulation mammary gland milk production nonhuman pregnancy priority journal protein expression rat Activin Receptors Activins Animals Caseins Cell Differentiation Cell Proliferation Female Follistatin Gene Expression Regulation Inhibin-beta Subunits Inhibins Lactation Mammary Glands, Animal Pregnancy Rats Rats, Sprague-Dawley RNA, Messenger Transforming Growth Factor beta Mammary gland morphogenesis and differentiation are mediated through the combined activities of systemic hormones and locally synthesized growth factors. Activin, a member of the transforming growth factor (TGF)-β superfamily, is known to regulate the growth and differentiation of several cell types. In the present study, we investigated the role of activin in rat mammary gland on different stages of development. We found that activin A in vitro inhibits the proliferation of isolated acini, and this effect increases with the development of the gland. This factor also produces in vitro an inhibition of the final differentiation of acini obtained from 19th day pregnant rats. We also report the expression of activin receptors IIA and IIB mRNA in whole rat mammary gland and acini, with decreased levels of expression of type IIA (in both compartments) and IIB (in acini) during pregnancy and lactogenesis. In addition, we show that activin βB-subunit mRNA decreases throughout pregnancy, and that the mRNA levels of follistatin (Fst) (its ligand protein) are high in cycling rats and at the beginning of pregnancy and diminish thereafter, having the acini higher levels of expression. Our data show that activin βB-subunit, follistatin and ActRIIA and IIB transcripts are expressed in rat mammary gland at appropriate times and locations during development, allowing an interplay that might regulate activin action on growth and differentiation of the gland. © 2004 Elsevier Ireland Ltd. All rights reserved. Fil:Bussmann, U.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lanuza, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03037207_v221_n1-2_p9_Bussmann |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Differentiation Growth Growth factors Lactogenesis Pregnancy activin activin A activin receptor activin receptor 2a activin receptor 2b follistatin ligand messenger RNA myelopid unclassified drug acinar cell animal experiment article cell differentiation cell isolation cell proliferation controlled study female growth regulation mammary gland milk production nonhuman pregnancy priority journal protein expression rat Activin Receptors Activins Animals Caseins Cell Differentiation Cell Proliferation Female Follistatin Gene Expression Regulation Inhibin-beta Subunits Inhibins Lactation Mammary Glands, Animal Pregnancy Rats Rats, Sprague-Dawley RNA, Messenger Transforming Growth Factor beta |
| spellingShingle |
Differentiation Growth Growth factors Lactogenesis Pregnancy activin activin A activin receptor activin receptor 2a activin receptor 2b follistatin ligand messenger RNA myelopid unclassified drug acinar cell animal experiment article cell differentiation cell isolation cell proliferation controlled study female growth regulation mammary gland milk production nonhuman pregnancy priority journal protein expression rat Activin Receptors Activins Animals Caseins Cell Differentiation Cell Proliferation Female Follistatin Gene Expression Regulation Inhibin-beta Subunits Inhibins Lactation Mammary Glands, Animal Pregnancy Rats Rats, Sprague-Dawley RNA, Messenger Transforming Growth Factor beta Bussmann, U.A. Lanuza, G.M. Bussmann, L.E. Activin and follistatin in rat mammary gland |
| topic_facet |
Differentiation Growth Growth factors Lactogenesis Pregnancy activin activin A activin receptor activin receptor 2a activin receptor 2b follistatin ligand messenger RNA myelopid unclassified drug acinar cell animal experiment article cell differentiation cell isolation cell proliferation controlled study female growth regulation mammary gland milk production nonhuman pregnancy priority journal protein expression rat Activin Receptors Activins Animals Caseins Cell Differentiation Cell Proliferation Female Follistatin Gene Expression Regulation Inhibin-beta Subunits Inhibins Lactation Mammary Glands, Animal Pregnancy Rats Rats, Sprague-Dawley RNA, Messenger Transforming Growth Factor beta |
| description |
Mammary gland morphogenesis and differentiation are mediated through the combined activities of systemic hormones and locally synthesized growth factors. Activin, a member of the transforming growth factor (TGF)-β superfamily, is known to regulate the growth and differentiation of several cell types. In the present study, we investigated the role of activin in rat mammary gland on different stages of development. We found that activin A in vitro inhibits the proliferation of isolated acini, and this effect increases with the development of the gland. This factor also produces in vitro an inhibition of the final differentiation of acini obtained from 19th day pregnant rats. We also report the expression of activin receptors IIA and IIB mRNA in whole rat mammary gland and acini, with decreased levels of expression of type IIA (in both compartments) and IIB (in acini) during pregnancy and lactogenesis. In addition, we show that activin βB-subunit mRNA decreases throughout pregnancy, and that the mRNA levels of follistatin (Fst) (its ligand protein) are high in cycling rats and at the beginning of pregnancy and diminish thereafter, having the acini higher levels of expression. Our data show that activin βB-subunit, follistatin and ActRIIA and IIB transcripts are expressed in rat mammary gland at appropriate times and locations during development, allowing an interplay that might regulate activin action on growth and differentiation of the gland. © 2004 Elsevier Ireland Ltd. All rights reserved. |
| format |
JOUR |
| author |
Bussmann, U.A. Lanuza, G.M. Bussmann, L.E. |
| author_facet |
Bussmann, U.A. Lanuza, G.M. Bussmann, L.E. |
| author_sort |
Bussmann, U.A. |
| title |
Activin and follistatin in rat mammary gland |
| title_short |
Activin and follistatin in rat mammary gland |
| title_full |
Activin and follistatin in rat mammary gland |
| title_fullStr |
Activin and follistatin in rat mammary gland |
| title_full_unstemmed |
Activin and follistatin in rat mammary gland |
| title_sort |
activin and follistatin in rat mammary gland |
| url |
http://hdl.handle.net/20.500.12110/paper_03037207_v221_n1-2_p9_Bussmann |
| work_keys_str_mv |
AT bussmannua activinandfollistatininratmammarygland AT lanuzagm activinandfollistatininratmammarygland AT bussmannle activinandfollistatininratmammarygland |
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1782027481948094464 |