Transcriptional regulation of interleukin-6 in pituitary folliculo-stellate TtT/GF cells
Interleukin-6 (IL-6) secreted by pituitary folliculo stellate (FS) cells plays an important role in the control of pituitary function and proliferation. We demonstrate that in FS TtT/GF cells, estradiol (E2) inhibits dose dependently pituitary adenylate cyclase activating polypeptide (PACAP)-stimula...
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todo:paper_03037207_v201_n1-2_p47_CarbiaNagashima2023-10-03T15:19:47Z Transcriptional regulation of interleukin-6 in pituitary folliculo-stellate TtT/GF cells Carbia Nagashima, A.C. Giacomini, D. Perez Castro, C. Paez Pereda, M. Renner, U. Stalla, G.K. Arzt, E. AP-1 CREB Estrogens Folliculo-stellate cells IL-6 PACAP estradiol hypophysis adenylate cyclase activating polypeptide interleukin 6 transcription factor transcription factor AP 1 transcription factor CREB transcription factor TRE unclassified drug animal cell article cell proliferation concentration response controlled study cytokine release hypophysis function mouse nonhuman point mutation priority journal stellate cell transcription initiation site transcription regulation Interleukin-6 (IL-6) secreted by pituitary folliculo stellate (FS) cells plays an important role in the control of pituitary function and proliferation. We demonstrate that in FS TtT/GF cells, estradiol (E2) inhibits dose dependently pituitary adenylate cyclase activating polypeptide (PACAP)-stimulated IL-6 secretion and transcription. We studied transcription factors involved in IL-6 stimulation by PACAP. Point mutations in κB, TRE, NF-IL-6 and CRE sites in the IL-6 promoter show that PACAP stimulates IL-6 through TRE and CRE sites. Accordingly, PACAP stimulated AP-1 and CREB transcriptional activity and E2 inhibited TRE-LUC but not CRE-LUC activation. Thus, we demonstrate that transcription factors of the CREB and AP-1 family are critical for the stimulation of IL-6 by PACAP in TtT/GF cells and that estrogens block this stimulation by inhibiting AP-1 activity. The regulatory elements involved in IL-6 transcription in TtT/GF FS cells contribute to understand the specificity of the anterior pituitary gland paracrine pathways. © 2003 Elsevier Science Ireland Ltd. All rights reserved. Fil:Carbia Nagashima, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giacomini, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paez Pereda, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03037207_v201_n1-2_p47_CarbiaNagashima |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
AP-1 CREB Estrogens Folliculo-stellate cells IL-6 PACAP estradiol hypophysis adenylate cyclase activating polypeptide interleukin 6 transcription factor transcription factor AP 1 transcription factor CREB transcription factor TRE unclassified drug animal cell article cell proliferation concentration response controlled study cytokine release hypophysis function mouse nonhuman point mutation priority journal stellate cell transcription initiation site transcription regulation |
| spellingShingle |
AP-1 CREB Estrogens Folliculo-stellate cells IL-6 PACAP estradiol hypophysis adenylate cyclase activating polypeptide interleukin 6 transcription factor transcription factor AP 1 transcription factor CREB transcription factor TRE unclassified drug animal cell article cell proliferation concentration response controlled study cytokine release hypophysis function mouse nonhuman point mutation priority journal stellate cell transcription initiation site transcription regulation Carbia Nagashima, A.C. Giacomini, D. Perez Castro, C. Paez Pereda, M. Renner, U. Stalla, G.K. Arzt, E. Transcriptional regulation of interleukin-6 in pituitary folliculo-stellate TtT/GF cells |
| topic_facet |
AP-1 CREB Estrogens Folliculo-stellate cells IL-6 PACAP estradiol hypophysis adenylate cyclase activating polypeptide interleukin 6 transcription factor transcription factor AP 1 transcription factor CREB transcription factor TRE unclassified drug animal cell article cell proliferation concentration response controlled study cytokine release hypophysis function mouse nonhuman point mutation priority journal stellate cell transcription initiation site transcription regulation |
| description |
Interleukin-6 (IL-6) secreted by pituitary folliculo stellate (FS) cells plays an important role in the control of pituitary function and proliferation. We demonstrate that in FS TtT/GF cells, estradiol (E2) inhibits dose dependently pituitary adenylate cyclase activating polypeptide (PACAP)-stimulated IL-6 secretion and transcription. We studied transcription factors involved in IL-6 stimulation by PACAP. Point mutations in κB, TRE, NF-IL-6 and CRE sites in the IL-6 promoter show that PACAP stimulates IL-6 through TRE and CRE sites. Accordingly, PACAP stimulated AP-1 and CREB transcriptional activity and E2 inhibited TRE-LUC but not CRE-LUC activation. Thus, we demonstrate that transcription factors of the CREB and AP-1 family are critical for the stimulation of IL-6 by PACAP in TtT/GF cells and that estrogens block this stimulation by inhibiting AP-1 activity. The regulatory elements involved in IL-6 transcription in TtT/GF FS cells contribute to understand the specificity of the anterior pituitary gland paracrine pathways. © 2003 Elsevier Science Ireland Ltd. All rights reserved. |
| format |
JOUR |
| author |
Carbia Nagashima, A.C. Giacomini, D. Perez Castro, C. Paez Pereda, M. Renner, U. Stalla, G.K. Arzt, E. |
| author_facet |
Carbia Nagashima, A.C. Giacomini, D. Perez Castro, C. Paez Pereda, M. Renner, U. Stalla, G.K. Arzt, E. |
| author_sort |
Carbia Nagashima, A.C. |
| title |
Transcriptional regulation of interleukin-6 in pituitary folliculo-stellate TtT/GF cells |
| title_short |
Transcriptional regulation of interleukin-6 in pituitary folliculo-stellate TtT/GF cells |
| title_full |
Transcriptional regulation of interleukin-6 in pituitary folliculo-stellate TtT/GF cells |
| title_fullStr |
Transcriptional regulation of interleukin-6 in pituitary folliculo-stellate TtT/GF cells |
| title_full_unstemmed |
Transcriptional regulation of interleukin-6 in pituitary folliculo-stellate TtT/GF cells |
| title_sort |
transcriptional regulation of interleukin-6 in pituitary folliculo-stellate ttt/gf cells |
| url |
http://hdl.handle.net/20.500.12110/paper_03037207_v201_n1-2_p47_CarbiaNagashima |
| work_keys_str_mv |
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