Isolation and characterization of specific rat epididymal proteins

The partial purification and characterization of specific rat epididymal proteins (SEP) is reported. Starting from the cytosol fraction obtained from epididymal homogenates, protein C was purified 15-fold and proteins D-E were purified 19-fold. The molecular weight, determined by molecular sieving,...

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Autores principales:	Garberi, J.C., Kohane, A.C., Cameo, M.S., Blaquier, J.A.
Formato:	JOUR
Materias:	sperm maturation glycoprotein protein animal article epididymis immunodiffusion isoelectric focusing male molecular weight rat Animal Epididymis Glycoproteins Immunodiffusion Isoelectric Focusing Male Molecular Weight Proteins Rats
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03037207_v13_n1_p73_Garberi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_03037207_v13_n1_p73_Garberi2023-10-03T15:19:44Z Isolation and characterization of specific rat epididymal proteins Garberi, J.C. Kohane, A.C. Cameo, M.S. Blaquier, J.A. sperm maturation glycoprotein protein animal article epididymis immunodiffusion isoelectric focusing male molecular weight rat Animal Epididymis Glycoproteins Immunodiffusion Isoelectric Focusing Male Molecular Weight Proteins Rats The partial purification and characterization of specific rat epididymal proteins (SEP) is reported. Starting from the cytosol fraction obtained from epididymal homogenates, protein C was purified 15-fold and proteins D-E were purified 19-fold. The molecular weight, determined by molecular sieving, of protein C was 22 400 while that of D-E was 37 000. These proteins stained as glycoproteins with periodic acid-Schiff reagent. The isoelectric point of protein D was 5.13 while that of protein E was 4.95. Protein C separated into 3 bands during isoelectric focussing. The major component focussed at 5.56 and the two minor components at pH 5.38 and 5.79. Using a specific antiserum we could confirm the organ specificity of SEP and their androgen-dependence. © 1979. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03037207_v13_n1_p73_Garberi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	sperm maturation glycoprotein protein animal article epididymis immunodiffusion isoelectric focusing male molecular weight rat Animal Epididymis Glycoproteins Immunodiffusion Isoelectric Focusing Male Molecular Weight Proteins Rats
spellingShingle	sperm maturation glycoprotein protein animal article epididymis immunodiffusion isoelectric focusing male molecular weight rat Animal Epididymis Glycoproteins Immunodiffusion Isoelectric Focusing Male Molecular Weight Proteins Rats Garberi, J.C. Kohane, A.C. Cameo, M.S. Blaquier, J.A. Isolation and characterization of specific rat epididymal proteins
topic_facet	sperm maturation glycoprotein protein animal article epididymis immunodiffusion isoelectric focusing male molecular weight rat Animal Epididymis Glycoproteins Immunodiffusion Isoelectric Focusing Male Molecular Weight Proteins Rats
description	The partial purification and characterization of specific rat epididymal proteins (SEP) is reported. Starting from the cytosol fraction obtained from epididymal homogenates, protein C was purified 15-fold and proteins D-E were purified 19-fold. The molecular weight, determined by molecular sieving, of protein C was 22 400 while that of D-E was 37 000. These proteins stained as glycoproteins with periodic acid-Schiff reagent. The isoelectric point of protein D was 5.13 while that of protein E was 4.95. Protein C separated into 3 bands during isoelectric focussing. The major component focussed at 5.56 and the two minor components at pH 5.38 and 5.79. Using a specific antiserum we could confirm the organ specificity of SEP and their androgen-dependence. © 1979.
format	JOUR
author	Garberi, J.C. Kohane, A.C. Cameo, M.S. Blaquier, J.A.
author_facet	Garberi, J.C. Kohane, A.C. Cameo, M.S. Blaquier, J.A.
author_sort	Garberi, J.C.
title	Isolation and characterization of specific rat epididymal proteins
title_short	Isolation and characterization of specific rat epididymal proteins
title_full	Isolation and characterization of specific rat epididymal proteins
title_fullStr	Isolation and characterization of specific rat epididymal proteins
title_full_unstemmed	Isolation and characterization of specific rat epididymal proteins
title_sort	isolation and characterization of specific rat epididymal proteins
url	http://hdl.handle.net/20.500.12110/paper_03037207_v13_n1_p73_Garberi
work_keys_str_mv	AT garberijc isolationandcharacterizationofspecificratepididymalproteins AT kohaneac isolationandcharacterizationofspecificratepididymalproteins AT cameoms isolationandcharacterizationofspecificratepididymalproteins AT blaquierja isolationandcharacterizationofspecificratepididymalproteins
_version_	1782026449716248576

Isolation and characterization of specific rat epididymal proteins

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