Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii
Inner membranes of Rhizobium leguminosarum biovars viciae, phaseoli, and trifolii synthesized a heterogenous family of neutral cyclic β-(1,2)-glucans in a reaction system that used oligosaccharide intermediates covalently bound to a large protein. This glucoprotein showed a slightly lower mobility o...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03028933_v163_n6_p454_Castro |
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todo:paper_03028933_v163_n6_p454_Castro2023-10-03T15:18:43Z Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii Castro, O.A. Zorreguieta, A. Semino, C. Ielpi, L. Biovar phaseoli Biovar trifolii Biovar viciae Cyclic β-(1,2)-glucan Glucoprotein intermediate Polysaccharide synthesis Rhizobium leguminosarum biovars beta glucan glycerophosphate glycoprotein oligosaccharide article bacterial membrane bacterial metabolism carbohydrate synthesis priority journal Rhizobium leguminosarum Rhizobium radiobacter Rhizobium trifolii Sinorhizobium meliloti Agrobacterium tumefaciens Bacteria (microorganisms) Rhizobium leguminosarum Sinorhizobium meliloti Inner membranes of Rhizobium leguminosarum biovars viciae, phaseoli, and trifolii synthesized a heterogenous family of neutral cyclic β-(1,2)-glucans in a reaction system that used oligosaccharide intermediates covalently bound to a large protein. This glucoprotein showed a slightly lower mobility on SDS-polyacrylamide gels (apparent mol. mass of 320 kDa) than the β-(1,2)-glucan intermediate protein of Rhizobium meliloti. In vivo pulse-label experiments with growing cells of R. leguminosarum biovar trifolii RS800 using radioactive glucose showed that few species of cyclic β-(1,2)-glucans were synthesized and up to 30% were substituted with charged non-glycosidic residues, probably sn-1-phosphoglycerol. © 1995 Springer-Verlag. Fil:Castro, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Zorreguieta, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Semino, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03028933_v163_n6_p454_Castro |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biovar phaseoli Biovar trifolii Biovar viciae Cyclic β-(1,2)-glucan Glucoprotein intermediate Polysaccharide synthesis Rhizobium leguminosarum biovars beta glucan glycerophosphate glycoprotein oligosaccharide article bacterial membrane bacterial metabolism carbohydrate synthesis priority journal Rhizobium leguminosarum Rhizobium radiobacter Rhizobium trifolii Sinorhizobium meliloti Agrobacterium tumefaciens Bacteria (microorganisms) Rhizobium leguminosarum Sinorhizobium meliloti |
| spellingShingle |
Biovar phaseoli Biovar trifolii Biovar viciae Cyclic β-(1,2)-glucan Glucoprotein intermediate Polysaccharide synthesis Rhizobium leguminosarum biovars beta glucan glycerophosphate glycoprotein oligosaccharide article bacterial membrane bacterial metabolism carbohydrate synthesis priority journal Rhizobium leguminosarum Rhizobium radiobacter Rhizobium trifolii Sinorhizobium meliloti Agrobacterium tumefaciens Bacteria (microorganisms) Rhizobium leguminosarum Sinorhizobium meliloti Castro, O.A. Zorreguieta, A. Semino, C. Ielpi, L. Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii |
| topic_facet |
Biovar phaseoli Biovar trifolii Biovar viciae Cyclic β-(1,2)-glucan Glucoprotein intermediate Polysaccharide synthesis Rhizobium leguminosarum biovars beta glucan glycerophosphate glycoprotein oligosaccharide article bacterial membrane bacterial metabolism carbohydrate synthesis priority journal Rhizobium leguminosarum Rhizobium radiobacter Rhizobium trifolii Sinorhizobium meliloti Agrobacterium tumefaciens Bacteria (microorganisms) Rhizobium leguminosarum Sinorhizobium meliloti |
| description |
Inner membranes of Rhizobium leguminosarum biovars viciae, phaseoli, and trifolii synthesized a heterogenous family of neutral cyclic β-(1,2)-glucans in a reaction system that used oligosaccharide intermediates covalently bound to a large protein. This glucoprotein showed a slightly lower mobility on SDS-polyacrylamide gels (apparent mol. mass of 320 kDa) than the β-(1,2)-glucan intermediate protein of Rhizobium meliloti. In vivo pulse-label experiments with growing cells of R. leguminosarum biovar trifolii RS800 using radioactive glucose showed that few species of cyclic β-(1,2)-glucans were synthesized and up to 30% were substituted with charged non-glycosidic residues, probably sn-1-phosphoglycerol. © 1995 Springer-Verlag. |
| format |
JOUR |
| author |
Castro, O.A. Zorreguieta, A. Semino, C. Ielpi, L. |
| author_facet |
Castro, O.A. Zorreguieta, A. Semino, C. Ielpi, L. |
| author_sort |
Castro, O.A. |
| title |
Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii |
| title_short |
Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii |
| title_full |
Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii |
| title_fullStr |
Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii |
| title_full_unstemmed |
Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii |
| title_sort |
biosynthesis of cyclic β-(1,2)-glucans in rhizobium leguminosarum biovars viciae, phaseoli and trifolii |
| url |
http://hdl.handle.net/20.500.12110/paper_03028933_v163_n6_p454_Castro |
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1807324359326433280 |