Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract
Rhodopseudomonas viridis was grown in the presence and in the absence of yeast extract. The cells grown under this latter condition present a ten fold diminished bacteriochlorophyll (bchl) content. This decrease was paralleled by a similar increase in the ratio lipid phosphorous/bchl, whereas the or...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03028933_v101_n1_p259_Pucheu |
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todo:paper_03028933_v101_n1_p259_Pucheu2023-10-03T15:18:42Z Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract Pucheu, N.L. Kerber, N.L. García, A.F. Lipid and Protein Composition Photosynthetic Membrane Reaction Center bacteriochlorophyll lipid ornithine protein bacterial cell wall in vitro study microorganism rhodopseudomonas viridis theoretical study Bacterial Proteins Cell Fractionation Cell Membrane Chlorophyll Chromatography, Thin Layer Comparative Study Culture Media Electrophoresis Fatty Acids Lipids Ornithine Phosphorus Rhodopseudomonas Saccharomyces cerevisiae Rhodopseudomonas viridis was grown in the presence and in the absence of yeast extract. The cells grown under this latter condition present a ten fold diminished bacteriochlorophyll (bchl) content. This decrease was paralleled by a similar increase in the ratio lipid phosphorous/bchl, whereas the ornithine lipid/bchl ratio remains constant. Some quantitative differences in the fatty acid composition are also reported. The protein composition of both membranes was also studied, only indicating quantitative differences. An active reaction center preparation was obtained from both types of cells. When isolated from cells grown in the presence of yeast extract, this reaction center preparation shows the presence of proteins a, b, c and d. Further treatment of this active reaction center results, in cells grown under either condition, in the isolation of green (oxidized behl) and brown (inactive reaction center) bchl containing fractions. The protein composition and absorption spectrum of the inactive reaction centers obtained from both types of cells were identical (proteins a, c and d). On the other hand the green complexes differ in their protein composition as well as in their absorption spectrum. © 1974 Springer-Verlag. Fil:Pucheu, N.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kerber, N.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:García, A.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03028933_v101_n1_p259_Pucheu |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Lipid and Protein Composition Photosynthetic Membrane Reaction Center bacteriochlorophyll lipid ornithine protein bacterial cell wall in vitro study microorganism rhodopseudomonas viridis theoretical study Bacterial Proteins Cell Fractionation Cell Membrane Chlorophyll Chromatography, Thin Layer Comparative Study Culture Media Electrophoresis Fatty Acids Lipids Ornithine Phosphorus Rhodopseudomonas Saccharomyces cerevisiae |
| spellingShingle |
Lipid and Protein Composition Photosynthetic Membrane Reaction Center bacteriochlorophyll lipid ornithine protein bacterial cell wall in vitro study microorganism rhodopseudomonas viridis theoretical study Bacterial Proteins Cell Fractionation Cell Membrane Chlorophyll Chromatography, Thin Layer Comparative Study Culture Media Electrophoresis Fatty Acids Lipids Ornithine Phosphorus Rhodopseudomonas Saccharomyces cerevisiae Pucheu, N.L. Kerber, N.L. García, A.F. Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract |
| topic_facet |
Lipid and Protein Composition Photosynthetic Membrane Reaction Center bacteriochlorophyll lipid ornithine protein bacterial cell wall in vitro study microorganism rhodopseudomonas viridis theoretical study Bacterial Proteins Cell Fractionation Cell Membrane Chlorophyll Chromatography, Thin Layer Comparative Study Culture Media Electrophoresis Fatty Acids Lipids Ornithine Phosphorus Rhodopseudomonas Saccharomyces cerevisiae |
| description |
Rhodopseudomonas viridis was grown in the presence and in the absence of yeast extract. The cells grown under this latter condition present a ten fold diminished bacteriochlorophyll (bchl) content. This decrease was paralleled by a similar increase in the ratio lipid phosphorous/bchl, whereas the ornithine lipid/bchl ratio remains constant. Some quantitative differences in the fatty acid composition are also reported. The protein composition of both membranes was also studied, only indicating quantitative differences. An active reaction center preparation was obtained from both types of cells. When isolated from cells grown in the presence of yeast extract, this reaction center preparation shows the presence of proteins a, b, c and d. Further treatment of this active reaction center results, in cells grown under either condition, in the isolation of green (oxidized behl) and brown (inactive reaction center) bchl containing fractions. The protein composition and absorption spectrum of the inactive reaction centers obtained from both types of cells were identical (proteins a, c and d). On the other hand the green complexes differ in their protein composition as well as in their absorption spectrum. © 1974 Springer-Verlag. |
| format |
JOUR |
| author |
Pucheu, N.L. Kerber, N.L. García, A.F. |
| author_facet |
Pucheu, N.L. Kerber, N.L. García, A.F. |
| author_sort |
Pucheu, N.L. |
| title |
Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract |
| title_short |
Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract |
| title_full |
Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract |
| title_fullStr |
Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract |
| title_full_unstemmed |
Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract |
| title_sort |
comparative studies on membranes isolated from rhodopseudomonas viridis grown in the presence and in the absence of yeast extract |
| url |
http://hdl.handle.net/20.500.12110/paper_03028933_v101_n1_p259_Pucheu |
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