Polyamines and antibiotic effects on translation

Protein synthesis was studied in polyamine-auxotrophic mutants of E. coli. The decreased protein synthesizing rate observed both in vivo and in vitro in polyamine-starved bacteria is due to defective 30S ribosomal subparticles which are impaired in the initiation step of translation. Analysis of pep...

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Autores principales:	Goldemberg, S.H., Algranati, I.D.
Formato:	JOUR
Materias:	kanamycin kasugamycin neomycin polyamine putrescine radioisotope spermidine spermine streptomycin tetracycline cell malformation dihydrostreptomycin h 3 dose response drug comparison drug response escherichia coli in vitro study leucine c 14 methionine s 35 phenylalanine c 14 protein synthesis Aminoglycosides Antibiotics Bacterial Proteins Cell-Free System Escherichia coli Polyamines Putrescine Ribosomes RNA, Transfer, Amino Acyl Streptomycin Support, Non-U.S. Gov't Translation, Genetic
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03022137_v59_n5-6_p360_Goldemberg
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03022137_v59_n5-6_p360_Goldemberg
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spelling	todo:paper_03022137_v59_n5-6_p360_Goldemberg2023-10-03T15:18:39Z Polyamines and antibiotic effects on translation Goldemberg, S.H. Algranati, I.D. kanamycin kasugamycin neomycin polyamine putrescine radioisotope spermidine spermine streptomycin tetracycline cell malformation dihydrostreptomycin h 3 dose response drug comparison drug response escherichia coli in vitro study leucine c 14 methionine s 35 phenylalanine c 14 protein synthesis Aminoglycosides Antibiotics Bacterial Proteins Cell-Free System Escherichia coli Polyamines Putrescine Ribosomes RNA, Transfer, Amino Acyl Streptomycin Support, Non-U.S. Gov't Translation, Genetic Protein synthesis was studied in polyamine-auxotrophic mutants of E. coli. The decreased protein synthesizing rate observed both in vivo and in vitro in polyamine-starved bacteria is due to defective 30S ribosomal subparticles which are impaired in the initiation step of translation. Analysis of peptides synthesized in vivo suggests a more extensive misreading by putrescine-depleted bacteria. Cells grown without polyamine show a markedly decreased response to the inhibitory effect and the misreading inducing action of streptomycin in vivo and in vitro. The polyamine-starved bacteria are also less sensitive to other aminoglycoside antibiotics such as neomycin, kanamycin and kasugamycin. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03022137_v59_n5-6_p360_Goldemberg
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	kanamycin kasugamycin neomycin polyamine putrescine radioisotope spermidine spermine streptomycin tetracycline cell malformation dihydrostreptomycin h 3 dose response drug comparison drug response escherichia coli in vitro study leucine c 14 methionine s 35 phenylalanine c 14 protein synthesis Aminoglycosides Antibiotics Bacterial Proteins Cell-Free System Escherichia coli Polyamines Putrescine Ribosomes RNA, Transfer, Amino Acyl Streptomycin Support, Non-U.S. Gov't Translation, Genetic
spellingShingle	kanamycin kasugamycin neomycin polyamine putrescine radioisotope spermidine spermine streptomycin tetracycline cell malformation dihydrostreptomycin h 3 dose response drug comparison drug response escherichia coli in vitro study leucine c 14 methionine s 35 phenylalanine c 14 protein synthesis Aminoglycosides Antibiotics Bacterial Proteins Cell-Free System Escherichia coli Polyamines Putrescine Ribosomes RNA, Transfer, Amino Acyl Streptomycin Support, Non-U.S. Gov't Translation, Genetic Goldemberg, S.H. Algranati, I.D. Polyamines and antibiotic effects on translation
topic_facet	kanamycin kasugamycin neomycin polyamine putrescine radioisotope spermidine spermine streptomycin tetracycline cell malformation dihydrostreptomycin h 3 dose response drug comparison drug response escherichia coli in vitro study leucine c 14 methionine s 35 phenylalanine c 14 protein synthesis Aminoglycosides Antibiotics Bacterial Proteins Cell-Free System Escherichia coli Polyamines Putrescine Ribosomes RNA, Transfer, Amino Acyl Streptomycin Support, Non-U.S. Gov't Translation, Genetic
description	Protein synthesis was studied in polyamine-auxotrophic mutants of E. coli. The decreased protein synthesizing rate observed both in vivo and in vitro in polyamine-starved bacteria is due to defective 30S ribosomal subparticles which are impaired in the initiation step of translation. Analysis of peptides synthesized in vivo suggests a more extensive misreading by putrescine-depleted bacteria. Cells grown without polyamine show a markedly decreased response to the inhibitory effect and the misreading inducing action of streptomycin in vivo and in vitro. The polyamine-starved bacteria are also less sensitive to other aminoglycoside antibiotics such as neomycin, kanamycin and kasugamycin.
format	JOUR
author	Goldemberg, S.H. Algranati, I.D.
author_facet	Goldemberg, S.H. Algranati, I.D.
author_sort	Goldemberg, S.H.
title	Polyamines and antibiotic effects on translation
title_short	Polyamines and antibiotic effects on translation
title_full	Polyamines and antibiotic effects on translation
title_fullStr	Polyamines and antibiotic effects on translation
title_full_unstemmed	Polyamines and antibiotic effects on translation
title_sort	polyamines and antibiotic effects on translation
url	http://hdl.handle.net/20.500.12110/paper_03022137_v59_n5-6_p360_Goldemberg
work_keys_str_mv	AT goldembergsh polyaminesandantibioticeffectsontranslation AT algranatiid polyaminesandantibioticeffectsontranslation
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Polyamines and antibiotic effects on translation

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