Cyclic AMP-dependent protein kinase from Ustilago maydis

Ustilago maydis was surveyed for cyclic AMP-dependent protein kinase activity. Using a combination of ion-exchange and molecular filtration techniques, we demonstrate that there is only one form of cyclic AMP-dependent protein kinase in the cytosolic fraction of the fungus. The kinase activity is sp...

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Detalles Bibliográficos
Autores principales: Kerner, N., Passeron, S.
Formato: JOUR
Materias:
cyclic AMP
protein kinase
article
Basidiomycetes
comparative study
density gradient centrifugation
drug effect
enzymology
isolation and purification
metabolism
molecular weight
protein conformation
Ustilago
Basidiomycota
Centrifugation, Density Gradient
Comparative Study
Cyclic AMP
Molecular Weight
Protein Conformation
Protein Kinases
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03008177_v60_n2_p115_Kerner
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Ustilago maydis was surveyed for cyclic AMP-dependent protein kinase activity. Using a combination of ion-exchange and molecular filtration techniques, we demonstrate that there is only one form of cyclic AMP-dependent protein kinase in the cytosolic fraction of the fungus. The kinase activity is specifically activated by cyclic AMP and utilizes protamine and kemptide as substrates. Most, if not all, of the cyclic AMP binding detected in the soluble fraction is associated with the protein kinase activity. Cyclic AMP-dependent protein kinase is completely dissociated by cyclic AMP into catalytic and regulatory subunits having an apparent molecular weight of 35 000 daltons as judged by sucrose gradient centrifugation. © 1984 Martinus Nijhoff Publishers.

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