Cyclic AMP-dependent protein kinase from Ustilago maydis

Ustilago maydis was surveyed for cyclic AMP-dependent protein kinase activity. Using a combination of ion-exchange and molecular filtration techniques, we demonstrate that there is only one form of cyclic AMP-dependent protein kinase in the cytosolic fraction of the fungus. The kinase activity is sp...

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Autores principales:	Kerner, N., Passeron, S.
Formato:	JOUR
Materias:	cyclic AMP protein kinase article Basidiomycetes comparative study density gradient centrifugation drug effect enzymology isolation and purification metabolism molecular weight protein conformation Ustilago Basidiomycota Centrifugation, Density Gradient Comparative Study Cyclic AMP Molecular Weight Protein Conformation Protein Kinases Support, Non-U.S. Gov't
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03008177_v60_n2_p115_Kerner
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03008177_v60_n2_p115_Kerner
record_format	dspace
spelling	todo:paper_03008177_v60_n2_p115_Kerner2023-10-03T15:17:52Z Cyclic AMP-dependent protein kinase from Ustilago maydis Kerner, N. Passeron, S. cyclic AMP protein kinase article Basidiomycetes comparative study density gradient centrifugation drug effect enzymology isolation and purification metabolism molecular weight protein conformation Ustilago Basidiomycota Centrifugation, Density Gradient Comparative Study Cyclic AMP Molecular Weight Protein Conformation Protein Kinases Support, Non-U.S. Gov't Ustilago Ustilago maydis was surveyed for cyclic AMP-dependent protein kinase activity. Using a combination of ion-exchange and molecular filtration techniques, we demonstrate that there is only one form of cyclic AMP-dependent protein kinase in the cytosolic fraction of the fungus. The kinase activity is specifically activated by cyclic AMP and utilizes protamine and kemptide as substrates. Most, if not all, of the cyclic AMP binding detected in the soluble fraction is associated with the protein kinase activity. Cyclic AMP-dependent protein kinase is completely dissociated by cyclic AMP into catalytic and regulatory subunits having an apparent molecular weight of 35 000 daltons as judged by sucrose gradient centrifugation. © 1984 Martinus Nijhoff Publishers. Fil:Kerner, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03008177_v60_n2_p115_Kerner
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cyclic AMP protein kinase article Basidiomycetes comparative study density gradient centrifugation drug effect enzymology isolation and purification metabolism molecular weight protein conformation Ustilago Basidiomycota Centrifugation, Density Gradient Comparative Study Cyclic AMP Molecular Weight Protein Conformation Protein Kinases Support, Non-U.S. Gov't Ustilago
spellingShingle	cyclic AMP protein kinase article Basidiomycetes comparative study density gradient centrifugation drug effect enzymology isolation and purification metabolism molecular weight protein conformation Ustilago Basidiomycota Centrifugation, Density Gradient Comparative Study Cyclic AMP Molecular Weight Protein Conformation Protein Kinases Support, Non-U.S. Gov't Ustilago Kerner, N. Passeron, S. Cyclic AMP-dependent protein kinase from Ustilago maydis
topic_facet	cyclic AMP protein kinase article Basidiomycetes comparative study density gradient centrifugation drug effect enzymology isolation and purification metabolism molecular weight protein conformation Ustilago Basidiomycota Centrifugation, Density Gradient Comparative Study Cyclic AMP Molecular Weight Protein Conformation Protein Kinases Support, Non-U.S. Gov't Ustilago
description	Ustilago maydis was surveyed for cyclic AMP-dependent protein kinase activity. Using a combination of ion-exchange and molecular filtration techniques, we demonstrate that there is only one form of cyclic AMP-dependent protein kinase in the cytosolic fraction of the fungus. The kinase activity is specifically activated by cyclic AMP and utilizes protamine and kemptide as substrates. Most, if not all, of the cyclic AMP binding detected in the soluble fraction is associated with the protein kinase activity. Cyclic AMP-dependent protein kinase is completely dissociated by cyclic AMP into catalytic and regulatory subunits having an apparent molecular weight of 35 000 daltons as judged by sucrose gradient centrifugation. © 1984 Martinus Nijhoff Publishers.
format	JOUR
author	Kerner, N. Passeron, S.
author_facet	Kerner, N. Passeron, S.
author_sort	Kerner, N.
title	Cyclic AMP-dependent protein kinase from Ustilago maydis
title_short	Cyclic AMP-dependent protein kinase from Ustilago maydis
title_full	Cyclic AMP-dependent protein kinase from Ustilago maydis
title_fullStr	Cyclic AMP-dependent protein kinase from Ustilago maydis
title_full_unstemmed	Cyclic AMP-dependent protein kinase from Ustilago maydis
title_sort	cyclic amp-dependent protein kinase from ustilago maydis
url	http://hdl.handle.net/20.500.12110/paper_03008177_v60_n2_p115_Kerner
work_keys_str_mv	AT kernern cyclicampdependentproteinkinasefromustilagomaydis AT passerons cyclicampdependentproteinkinasefromustilagomaydis
_version_	1782024020041924608

Cyclic AMP-dependent protein kinase from Ustilago maydis

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