Cyclic nucleotide phosphodiesterase activities in rat erythrocytes

Erythrocyte soluble phosphodiesterase activities show at least two interconvertible states: 'aggregated' and 'non-aggregated'. In the former state the existence of a high affinity component for cyclic AMP is favoured and the system is more active with cyclic AMP than with cyclic...

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Autores principales:	Téllez-Iñón, M.T., Torres, H.N.
Formato:	JOUR
Materias:	cyclic amp cyclic gmp cyclic nucleotide phosphodiesterase animal experiment blood and hemopoietic system erythrocyte rat 3',5'-Cyclic-GMP Phosphodiesterase 3',5'-Cyclic-Nucleotide Phosphodiesterase Animal Chromatography, Gel Erythrocytes Isoelectric Focusing Kinetics Magnesium Magnesium Chloride Male Rats Rats, Inbred Strains Support, Non-U.S. Gov't
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03008177_v43_n3_p161_TellezInon
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03008177_v43_n3_p161_TellezInon
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spelling	todo:paper_03008177_v43_n3_p161_TellezInon2023-10-03T15:17:49Z Cyclic nucleotide phosphodiesterase activities in rat erythrocytes Téllez-Iñón, M.T. Torres, H.N. cyclic amp cyclic gmp cyclic nucleotide phosphodiesterase animal experiment blood and hemopoietic system erythrocyte rat 3',5'-Cyclic-GMP Phosphodiesterase 3',5'-Cyclic-Nucleotide Phosphodiesterase Animal Chromatography, Gel Erythrocytes Isoelectric Focusing Kinetics Magnesium Magnesium Chloride Male Rats Rats, Inbred Strains Support, Non-U.S. Gov't Erythrocyte soluble phosphodiesterase activities show at least two interconvertible states: 'aggregated' and 'non-aggregated'. In the former state the existence of a high affinity component for cyclic AMP is favoured and the system is more active with cyclic AMP than with cyclic GMP as substrate. When the system is in the 'non-aggregated state', no activity with cyclic GMP is detected. Conversion to the 'non-aggregated state' is enhanced by dilution or by addition of magnesium ions. Upon isoelectric focusing of erythrocyte soluble extracts, phosphodiesterase activities can be resolved in two peaks: one specific for cyclic AMP located at pH 4.66 and the other specific for cyclic GMP focused at pH 4.95. Evidence would indicate that aggregation affects the enzyme specific for cyclic AMP. © 1982 Martinus Nijhoff/Dr W. Junk Publishers. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03008177_v43_n3_p161_TellezInon
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cyclic amp cyclic gmp cyclic nucleotide phosphodiesterase animal experiment blood and hemopoietic system erythrocyte rat 3',5'-Cyclic-GMP Phosphodiesterase 3',5'-Cyclic-Nucleotide Phosphodiesterase Animal Chromatography, Gel Erythrocytes Isoelectric Focusing Kinetics Magnesium Magnesium Chloride Male Rats Rats, Inbred Strains Support, Non-U.S. Gov't
spellingShingle	cyclic amp cyclic gmp cyclic nucleotide phosphodiesterase animal experiment blood and hemopoietic system erythrocyte rat 3',5'-Cyclic-GMP Phosphodiesterase 3',5'-Cyclic-Nucleotide Phosphodiesterase Animal Chromatography, Gel Erythrocytes Isoelectric Focusing Kinetics Magnesium Magnesium Chloride Male Rats Rats, Inbred Strains Support, Non-U.S. Gov't Téllez-Iñón, M.T. Torres, H.N. Cyclic nucleotide phosphodiesterase activities in rat erythrocytes
topic_facet	cyclic amp cyclic gmp cyclic nucleotide phosphodiesterase animal experiment blood and hemopoietic system erythrocyte rat 3',5'-Cyclic-GMP Phosphodiesterase 3',5'-Cyclic-Nucleotide Phosphodiesterase Animal Chromatography, Gel Erythrocytes Isoelectric Focusing Kinetics Magnesium Magnesium Chloride Male Rats Rats, Inbred Strains Support, Non-U.S. Gov't
description	Erythrocyte soluble phosphodiesterase activities show at least two interconvertible states: 'aggregated' and 'non-aggregated'. In the former state the existence of a high affinity component for cyclic AMP is favoured and the system is more active with cyclic AMP than with cyclic GMP as substrate. When the system is in the 'non-aggregated state', no activity with cyclic GMP is detected. Conversion to the 'non-aggregated state' is enhanced by dilution or by addition of magnesium ions. Upon isoelectric focusing of erythrocyte soluble extracts, phosphodiesterase activities can be resolved in two peaks: one specific for cyclic AMP located at pH 4.66 and the other specific for cyclic GMP focused at pH 4.95. Evidence would indicate that aggregation affects the enzyme specific for cyclic AMP. © 1982 Martinus Nijhoff/Dr W. Junk Publishers.
format	JOUR
author	Téllez-Iñón, M.T. Torres, H.N.
author_facet	Téllez-Iñón, M.T. Torres, H.N.
author_sort	Téllez-Iñón, M.T.
title	Cyclic nucleotide phosphodiesterase activities in rat erythrocytes
title_short	Cyclic nucleotide phosphodiesterase activities in rat erythrocytes
title_full	Cyclic nucleotide phosphodiesterase activities in rat erythrocytes
title_fullStr	Cyclic nucleotide phosphodiesterase activities in rat erythrocytes
title_full_unstemmed	Cyclic nucleotide phosphodiesterase activities in rat erythrocytes
title_sort	cyclic nucleotide phosphodiesterase activities in rat erythrocytes
url	http://hdl.handle.net/20.500.12110/paper_03008177_v43_n3_p161_TellezInon
work_keys_str_mv	AT tellezinonmt cyclicnucleotidephosphodiesteraseactivitiesinraterythrocytes AT torreshn cyclicnucleotidephosphodiesteraseactivitiesinraterythrocytes
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Cyclic nucleotide phosphodiesterase activities in rat erythrocytes

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