Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum

A particulate enzyme preparation from Acetobacter xylinum synthesizes ficaprenol-monophosphate-β-galactose from ficaprenol monophosphate (FMP) and UDP-galactose in the presence of detergent. The product has the same properties as those previously reported for the compound formed with the endogenous...

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Autores principales: Romero, P., Garcia, R.C., Dankert, M.
Formato: JOUR
Materias:
acetobacter xylinum
beta galactoside derivative
in vitro study
microorganism
polyprenol derivative
theoretical study
Acetobacter
Hexosephosphates
Kinetics
Polyethylene Glycols
Polysaccharides
Support, U.S. Gov't, P.H.S.
Terpenes
Uridine Diphosphate Galactose
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p205_Romero
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03008177_v16_n2-3_p205_Romero
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spelling todo:paper_03008177_v16_n2-3_p205_Romero2023-10-03T15:17:44Z Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum Romero, P. Garcia, R.C. Dankert, M. acetobacter xylinum beta galactoside derivative in vitro study microorganism polyprenol derivative theoretical study Acetobacter Hexosephosphates Kinetics Polyethylene Glycols Polysaccharides Support, U.S. Gov't, P.H.S. Terpenes Uridine Diphosphate Galactose A particulate enzyme preparation from Acetobacter xylinum synthesizes ficaprenol-monophosphate-β-galactose from ficaprenol monophosphate (FMP) and UDP-galactose in the presence of detergent. The product has the same properties as those previously reported for the compound formed with the endogenous acceptor. Dolichol-monophosphate (DolMP) is also a good galactose acceptor but the product obtained has different properties. Lipid extracts from Acetobacter contain galactose acceptor capacity which is lost by mild acid treatment. FMP behaves in a similar manner but DolMP is resistant to this treatment. It is concluded that the endogeneous acceptor is an allylic phosphate ester of a polyprenol. © 1977 Dr. W. Junk b.v. Publishers. Fil:Romero, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Garcia, R.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dankert, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p205_Romero
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic acetobacter xylinum
beta galactoside derivative
in vitro study
microorganism
polyprenol derivative
theoretical study
Acetobacter
Hexosephosphates
Kinetics
Polyethylene Glycols
Polysaccharides
Support, U.S. Gov't, P.H.S.
Terpenes
Uridine Diphosphate Galactose
spellingShingle acetobacter xylinum
beta galactoside derivative
in vitro study
microorganism
polyprenol derivative
theoretical study
Acetobacter
Hexosephosphates
Kinetics
Polyethylene Glycols
Polysaccharides
Support, U.S. Gov't, P.H.S.
Terpenes
Uridine Diphosphate Galactose
Romero, P.
Garcia, R.C.
Dankert, M.
Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum
topic_facet acetobacter xylinum
beta galactoside derivative
in vitro study
microorganism
polyprenol derivative
theoretical study
Acetobacter
Hexosephosphates
Kinetics
Polyethylene Glycols
Polysaccharides
Support, U.S. Gov't, P.H.S.
Terpenes
Uridine Diphosphate Galactose
description A particulate enzyme preparation from Acetobacter xylinum synthesizes ficaprenol-monophosphate-β-galactose from ficaprenol monophosphate (FMP) and UDP-galactose in the presence of detergent. The product has the same properties as those previously reported for the compound formed with the endogenous acceptor. Dolichol-monophosphate (DolMP) is also a good galactose acceptor but the product obtained has different properties. Lipid extracts from Acetobacter contain galactose acceptor capacity which is lost by mild acid treatment. FMP behaves in a similar manner but DolMP is resistant to this treatment. It is concluded that the endogeneous acceptor is an allylic phosphate ester of a polyprenol. © 1977 Dr. W. Junk b.v. Publishers.
format JOUR
author Romero, P.
Garcia, R.C.
Dankert, M.
author_facet Romero, P.
Garcia, R.C.
Dankert, M.
author_sort Romero, P.
title Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum
title_short Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum
title_full Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum
title_fullStr Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum
title_full_unstemmed Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum
title_sort synthesis of polyprenol-monophosphate-β-galactose by acetobacter xylinum
url http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p205_Romero
work_keys_str_mv AT romerop synthesisofpolyprenolmonophosphatebgalactosebyacetobacterxylinum
AT garciarc synthesisofpolyprenolmonophosphatebgalactosebyacetobacterxylinum
AT dankertm synthesisofpolyprenolmonophosphatebgalactosebyacetobacterxylinum
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