Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells

Incubation of cells from a wild type strain of E. coli with 0.3 mg/ml rifampicin for 15 minutes lead to a complete inhibition of RNA synthesis measured as the uracil incorporation into the trichloroacetic acid insoluble fraction. In these rifampicin-treated cells [14C]uracil incorporation tended to...

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Autores principales: Judewicz, N.D., Torres, H.N.
Formato: JOUR
Materias:
cyclic amp
radioisotope
rifampicin
rna
uracil
cell membrane transport
dose time effect relation
escherichia coli
in vitro study
microorganism
rna synthesis
uracil c 14
Adenosine Monophosphate
Adenosine Triphosphate
Biological Transport, Active
Cyclic AMP
Escherichia coli
Kinetics
Rifampin
RNA, Bacterial
Transcription, Genetic
Uracil
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p135_Judewicz
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03008177_v16_n2-3_p135_Judewicz
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spelling todo:paper_03008177_v16_n2-3_p135_Judewicz2023-10-03T15:17:42Z Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells Judewicz, N.D. Torres, H.N. cyclic amp radioisotope rifampicin rna uracil cell membrane transport dose time effect relation escherichia coli in vitro study microorganism rna synthesis uracil c 14 Adenosine Monophosphate Adenosine Triphosphate Biological Transport, Active Cyclic AMP Escherichia coli Kinetics Rifampin RNA, Bacterial Transcription, Genetic Uracil Incubation of cells from a wild type strain of E. coli with 0.3 mg/ml rifampicin for 15 minutes lead to a complete inhibition of RNA synthesis measured as the uracil incorporation into the trichloroacetic acid insoluble fraction. In these rifampicin-treated cells [14C]uracil incorporation tended to decrease during a further incubation at 37°. Addition of cyclic AMP increased the inactivation of the system responsible for [14C]uracil uptake. The cyclic nucleotide effect seems to be specific since ATP or 5′AMP did not increase such inactivation. © 1977 Dr. W. Junk b.v. Publishers. Fil:Judewicz, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p135_Judewicz
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cyclic amp
radioisotope
rifampicin
rna
uracil
cell membrane transport
dose time effect relation
escherichia coli
in vitro study
microorganism
rna synthesis
uracil c 14
Adenosine Monophosphate
Adenosine Triphosphate
Biological Transport, Active
Cyclic AMP
Escherichia coli
Kinetics
Rifampin
RNA, Bacterial
Transcription, Genetic
Uracil
spellingShingle cyclic amp
radioisotope
rifampicin
rna
uracil
cell membrane transport
dose time effect relation
escherichia coli
in vitro study
microorganism
rna synthesis
uracil c 14
Adenosine Monophosphate
Adenosine Triphosphate
Biological Transport, Active
Cyclic AMP
Escherichia coli
Kinetics
Rifampin
RNA, Bacterial
Transcription, Genetic
Uracil
Judewicz, N.D.
Torres, H.N.
Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells
topic_facet cyclic amp
radioisotope
rifampicin
rna
uracil
cell membrane transport
dose time effect relation
escherichia coli
in vitro study
microorganism
rna synthesis
uracil c 14
Adenosine Monophosphate
Adenosine Triphosphate
Biological Transport, Active
Cyclic AMP
Escherichia coli
Kinetics
Rifampin
RNA, Bacterial
Transcription, Genetic
Uracil
description Incubation of cells from a wild type strain of E. coli with 0.3 mg/ml rifampicin for 15 minutes lead to a complete inhibition of RNA synthesis measured as the uracil incorporation into the trichloroacetic acid insoluble fraction. In these rifampicin-treated cells [14C]uracil incorporation tended to decrease during a further incubation at 37°. Addition of cyclic AMP increased the inactivation of the system responsible for [14C]uracil uptake. The cyclic nucleotide effect seems to be specific since ATP or 5′AMP did not increase such inactivation. © 1977 Dr. W. Junk b.v. Publishers.
format JOUR
author Judewicz, N.D.
Torres, H.N.
author_facet Judewicz, N.D.
Torres, H.N.
author_sort Judewicz, N.D.
title Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells
title_short Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells
title_full Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells
title_fullStr Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells
title_full_unstemmed Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells
title_sort influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated escherichia coli cells
url http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p135_Judewicz
work_keys_str_mv AT judewicznd influenceofcyclic35adenosinemonophosphateonuraciluptakebyrifampicintreatedescherichiacolicells
AT torreshn influenceofcyclic35adenosinemonophosphateonuraciluptakebyrifampicintreatedescherichiacolicells
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