Interactions of Junin and Tacaribe viruses during mixed infections

The interaction between Junin virus (JV) and Tacaribe virus (TACV) during mixed infections of RK13 cells was examined. The effects of a prior infection with JV upon TACV replication depended on the time between the two inoculations. Simultaneous infection of RK13 cells with TACV and JV did not alter...

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Autores principales: Damonte, E.B., Coto, C.E.
Formato: JOUR
Materias:
cell culture
in vitro study
Junin virus
mixed virus infection
nonhuman
priority journal
tacaribe arenavirus
virus replication
Arenavirus
Tacaribe virus
Vesicular stomatitis virus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03005526_v23_n1_p1_Damonte
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03005526_v23_n1_p1_Damonte
record_format dspace
spelling todo:paper_03005526_v23_n1_p1_Damonte2023-10-03T15:17:39Z Interactions of Junin and Tacaribe viruses during mixed infections Damonte, E.B. Coto, C.E. cell culture in vitro study Junin virus mixed virus infection nonhuman priority journal tacaribe arenavirus virus replication Arenavirus Junin virus Tacaribe virus Vesicular stomatitis virus The interaction between Junin virus (JV) and Tacaribe virus (TACV) during mixed infections of RK13 cells was examined. The effects of a prior infection with JV upon TACV replication depended on the time between the two inoculations. Simultaneous infection of RK13 cells with TACV and JV did not alter the plaquing efficiency of TACV; but if there was a 1- to 24-hour delay between JV preinfection and TACV superinfection, a variable increase of TACV replication was observed. The enhancement of TACV replication by preinfection with JV was dependent on several factors, such as the MOI of both viruses and the integrity of the JV genome. This effect was also highly specific, as the plaquing efficiencies of the arenavirus Pichinde and the unrelated vesicular stomatitis virus were not affected by preinfection with JV at any multiplicity assayed. The majority of the progeny formed in cells superinfected with TACV 1 or 24 hr after JV infection was partially neutralized by antisera to both viruses. This suggested that phenotypic mixing, with JV or TACV genomes enclosed within an envelope containing TACV and JV glycoproteins, has occurred. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03005526_v23_n1_p1_Damonte
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cell culture
in vitro study
Junin virus
mixed virus infection
nonhuman
priority journal
tacaribe arenavirus
virus replication
Arenavirus
Junin virus
Tacaribe virus
Vesicular stomatitis virus
spellingShingle cell culture
in vitro study
Junin virus
mixed virus infection
nonhuman
priority journal
tacaribe arenavirus
virus replication
Arenavirus
Junin virus
Tacaribe virus
Vesicular stomatitis virus
Damonte, E.B.
Coto, C.E.
Interactions of Junin and Tacaribe viruses during mixed infections
topic_facet cell culture
in vitro study
Junin virus
mixed virus infection
nonhuman
priority journal
tacaribe arenavirus
virus replication
Arenavirus
Junin virus
Tacaribe virus
Vesicular stomatitis virus
description The interaction between Junin virus (JV) and Tacaribe virus (TACV) during mixed infections of RK13 cells was examined. The effects of a prior infection with JV upon TACV replication depended on the time between the two inoculations. Simultaneous infection of RK13 cells with TACV and JV did not alter the plaquing efficiency of TACV; but if there was a 1- to 24-hour delay between JV preinfection and TACV superinfection, a variable increase of TACV replication was observed. The enhancement of TACV replication by preinfection with JV was dependent on several factors, such as the MOI of both viruses and the integrity of the JV genome. This effect was also highly specific, as the plaquing efficiencies of the arenavirus Pichinde and the unrelated vesicular stomatitis virus were not affected by preinfection with JV at any multiplicity assayed. The majority of the progeny formed in cells superinfected with TACV 1 or 24 hr after JV infection was partially neutralized by antisera to both viruses. This suggested that phenotypic mixing, with JV or TACV genomes enclosed within an envelope containing TACV and JV glycoproteins, has occurred.
format JOUR
author Damonte, E.B.
Coto, C.E.
author_facet Damonte, E.B.
Coto, C.E.
author_sort Damonte, E.B.
title Interactions of Junin and Tacaribe viruses during mixed infections
title_short Interactions of Junin and Tacaribe viruses during mixed infections
title_full Interactions of Junin and Tacaribe viruses during mixed infections
title_fullStr Interactions of Junin and Tacaribe viruses during mixed infections
title_full_unstemmed Interactions of Junin and Tacaribe viruses during mixed infections
title_sort interactions of junin and tacaribe viruses during mixed infections
url http://hdl.handle.net/20.500.12110/paper_03005526_v23_n1_p1_Damonte
work_keys_str_mv AT damonteeb interactionsofjuninandtacaribevirusesduringmixedinfections
AT cotoce interactionsofjuninandtacaribevirusesduringmixedinfections
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