Relationship between exposure, body burden and target tissue concentration after oral administration of a low-dose mixture of pyrethroid insecticides in young adult rats
Pyrethroids (PYRs) are synthetic insecticides increasingly used in agricultural and household pest control. Little is known on how the toxicity of highly effective bolus doses of single compounds compares to more realistic scenarios of low-level exposure to PYR mixtures. In this study, we examined a...
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todo:paper_0300483X_v409_n_p53_MosqueraOrtega2023-10-03T15:17:37Z Relationship between exposure, body burden and target tissue concentration after oral administration of a low-dose mixture of pyrethroid insecticides in young adult rats Mosquera Ortega, M.E. Romero, D.M. Pato, A.M. Sosa-Holt, C.S. Ridolfi, A. Villaamil Lepori, E. Wolansky, M.J. Body temperature Cumulative toxicity Mixture Pyrethroids Rat Toxicokinetics bifenthrin corn oil cypermethrin deltamethrin pyrethroid tefluthrin unclassified drug adult animal experiment animal model animal tissue Article body burden body temperature cerebellum controlled study gas chromatography lethality liver locomotion neurotoxicity nonhuman priority journal rat target tissue young adult Pyrethroids (PYRs) are synthetic insecticides increasingly used in agricultural and household pest control. Little is known on how the toxicity of highly effective bolus doses of single compounds compares to more realistic scenarios of low-level exposure to PYR mixtures. In this study, we examined a quaternary mixture of two noncyano (tefluthrin, TEF; bifenthrin, BIF) and two cyano (α-cypermethrin, α-CPM; deltamethrin, DTM) PYRs in young adult rats. These compounds are mostly composed of PYR isomers ranking top ten in acute lethality in rats. Concurrently, we administered near-threshold levels of the four PYRs dissolved in corn oil by oral route. Six hours later blood was collected and the liver and cerebellum were dissected out to determine PYR concentrations in these tissues using Gas Chromatography with Electron Capture Detector (GC-ECD). The mixture caused mild-to-moderate changes in non-locomotor behaviors and subcutaneous body temperature (up to +1.2–1.5 °C increase at 2–4 h after dosing, respectively, compared to pre-dosing records). The most toxic PYRs BIF and TEF reached higher concentrations in the cerebellum than the cyano-compounds α-CPM and DTM. In addition, PYR concentrations in the cerebellum were correlated to single compound proportions in the dosing solution and changes in body temperature. Our results suggest that aggregate exposures resulting in a target tissue burden of ∼10−1 nmoles PYR/g may be toxicologically relevant, expanding the evidence on exposure-dose-effect relationships for PYRs, and serving to design convenient pharmacokinetic models for environmentally relevant exposures to PYR mixtures. © 2018 JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0300483X_v409_n_p53_MosqueraOrtega |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Body temperature Cumulative toxicity Mixture Pyrethroids Rat Toxicokinetics bifenthrin corn oil cypermethrin deltamethrin pyrethroid tefluthrin unclassified drug adult animal experiment animal model animal tissue Article body burden body temperature cerebellum controlled study gas chromatography lethality liver locomotion neurotoxicity nonhuman priority journal rat target tissue young adult |
| spellingShingle |
Body temperature Cumulative toxicity Mixture Pyrethroids Rat Toxicokinetics bifenthrin corn oil cypermethrin deltamethrin pyrethroid tefluthrin unclassified drug adult animal experiment animal model animal tissue Article body burden body temperature cerebellum controlled study gas chromatography lethality liver locomotion neurotoxicity nonhuman priority journal rat target tissue young adult Mosquera Ortega, M.E. Romero, D.M. Pato, A.M. Sosa-Holt, C.S. Ridolfi, A. Villaamil Lepori, E. Wolansky, M.J. Relationship between exposure, body burden and target tissue concentration after oral administration of a low-dose mixture of pyrethroid insecticides in young adult rats |
| topic_facet |
Body temperature Cumulative toxicity Mixture Pyrethroids Rat Toxicokinetics bifenthrin corn oil cypermethrin deltamethrin pyrethroid tefluthrin unclassified drug adult animal experiment animal model animal tissue Article body burden body temperature cerebellum controlled study gas chromatography lethality liver locomotion neurotoxicity nonhuman priority journal rat target tissue young adult |
| description |
Pyrethroids (PYRs) are synthetic insecticides increasingly used in agricultural and household pest control. Little is known on how the toxicity of highly effective bolus doses of single compounds compares to more realistic scenarios of low-level exposure to PYR mixtures. In this study, we examined a quaternary mixture of two noncyano (tefluthrin, TEF; bifenthrin, BIF) and two cyano (α-cypermethrin, α-CPM; deltamethrin, DTM) PYRs in young adult rats. These compounds are mostly composed of PYR isomers ranking top ten in acute lethality in rats. Concurrently, we administered near-threshold levels of the four PYRs dissolved in corn oil by oral route. Six hours later blood was collected and the liver and cerebellum were dissected out to determine PYR concentrations in these tissues using Gas Chromatography with Electron Capture Detector (GC-ECD). The mixture caused mild-to-moderate changes in non-locomotor behaviors and subcutaneous body temperature (up to +1.2–1.5 °C increase at 2–4 h after dosing, respectively, compared to pre-dosing records). The most toxic PYRs BIF and TEF reached higher concentrations in the cerebellum than the cyano-compounds α-CPM and DTM. In addition, PYR concentrations in the cerebellum were correlated to single compound proportions in the dosing solution and changes in body temperature. Our results suggest that aggregate exposures resulting in a target tissue burden of ∼10−1 nmoles PYR/g may be toxicologically relevant, expanding the evidence on exposure-dose-effect relationships for PYRs, and serving to design convenient pharmacokinetic models for environmentally relevant exposures to PYR mixtures. © 2018 |
| format |
JOUR |
| author |
Mosquera Ortega, M.E. Romero, D.M. Pato, A.M. Sosa-Holt, C.S. Ridolfi, A. Villaamil Lepori, E. Wolansky, M.J. |
| author_facet |
Mosquera Ortega, M.E. Romero, D.M. Pato, A.M. Sosa-Holt, C.S. Ridolfi, A. Villaamil Lepori, E. Wolansky, M.J. |
| author_sort |
Mosquera Ortega, M.E. |
| title |
Relationship between exposure, body burden and target tissue concentration after oral administration of a low-dose mixture of pyrethroid insecticides in young adult rats |
| title_short |
Relationship between exposure, body burden and target tissue concentration after oral administration of a low-dose mixture of pyrethroid insecticides in young adult rats |
| title_full |
Relationship between exposure, body burden and target tissue concentration after oral administration of a low-dose mixture of pyrethroid insecticides in young adult rats |
| title_fullStr |
Relationship between exposure, body burden and target tissue concentration after oral administration of a low-dose mixture of pyrethroid insecticides in young adult rats |
| title_full_unstemmed |
Relationship between exposure, body burden and target tissue concentration after oral administration of a low-dose mixture of pyrethroid insecticides in young adult rats |
| title_sort |
relationship between exposure, body burden and target tissue concentration after oral administration of a low-dose mixture of pyrethroid insecticides in young adult rats |
| url |
http://hdl.handle.net/20.500.12110/paper_0300483X_v409_n_p53_MosqueraOrtega |
| work_keys_str_mv |
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