Heterogeneous distribution of G protein alpha subunits in the main olfactory and vomeronasal systems of Rhinella (Bufo) arenarum tadpoles.

We evaluated the presence of G protein subtypes Galpha(o), Galpha(i2), and Galpha(olf) in the main olfactory system (MOS) and accessory or vomeronasal system (VNS) of Rhinella (Bufo) arenarum tadpoles, and here describe the fine structure of the sensory cells in the olfactory epithelium (OE) and vom...

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Autores principales: Jungblut, L.D., Paz, D.A., López-Costa, J.J., Pozzi, A.G.
Formato: JOUR
Materias:
guanine nucleotide binding protein alpha subunit
animal
article
larva
metabolism
olfactory system
toad
vomeronasal organ
Animals
Bufonidae
GTP-Binding Protein alpha Subunits
Larva
Olfactory Pathways
Vomeronasal Organ
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02890003_v26_n10_p722_Jungblut
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_02890003_v26_n10_p722_Jungblut
record_format dspace
spelling todo:paper_02890003_v26_n10_p722_Jungblut2023-10-03T15:17:15Z Heterogeneous distribution of G protein alpha subunits in the main olfactory and vomeronasal systems of Rhinella (Bufo) arenarum tadpoles. Jungblut, L.D. Paz, D.A. López-Costa, J.J. Pozzi, A.G. guanine nucleotide binding protein alpha subunit animal article larva metabolism olfactory system toad vomeronasal organ Animals Bufonidae GTP-Binding Protein alpha Subunits Larva Olfactory Pathways Vomeronasal Organ We evaluated the presence of G protein subtypes Galpha(o), Galpha(i2), and Galpha(olf) in the main olfactory system (MOS) and accessory or vomeronasal system (VNS) of Rhinella (Bufo) arenarum tadpoles, and here describe the fine structure of the sensory cells in the olfactory epithelium (OE) and vomeronasal organ (VNO). The OE shows olfactory receptor neurons (ORNs) with cilia in the apical surface, and the vomeronasal receptor neurons (VRNs) of the VNO are covered with microvilli. Immunohistochemistry detected the presence of at least two segregated populations of ORNs throughout the OE, coupled to Galpha(olf) and Galpha(o). An antiserum against Galpha(i2) was ineffective in staining the ORNs. In the VNO, Galpha(o) neurons stained strongly but lacked immunoreactivity to any other Galpha subunit in all larval stages analyzed. Western blot analyses and preabsorption experiments confirmed the specificity of the commercial antisera used. The functional significance of the heterogeneous G-protein distribution in R. arenarum tadpoles is not clear, but the study of G- protein distributions in various amphibian species is important, since this vertebrate group played a key role in the evolution of tetrapods. A more complete knowledge of the amphibian MOS and VNS would help to understand the functional organization and evolution of vertebrate chemosensory systems. This work demonstrates, for the first time, the existence of a segregated distribution of G-proteins in the OE of R. arenarum tadpoles. Fil:Jungblut, L.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pozzi, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02890003_v26_n10_p722_Jungblut
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic guanine nucleotide binding protein alpha subunit
animal
article
larva
metabolism
olfactory system
toad
vomeronasal organ
Animals
Bufonidae
GTP-Binding Protein alpha Subunits
Larva
Olfactory Pathways
Vomeronasal Organ
spellingShingle guanine nucleotide binding protein alpha subunit
animal
article
larva
metabolism
olfactory system
toad
vomeronasal organ
Animals
Bufonidae
GTP-Binding Protein alpha Subunits
Larva
Olfactory Pathways
Vomeronasal Organ
Jungblut, L.D.
Paz, D.A.
López-Costa, J.J.
Pozzi, A.G.
Heterogeneous distribution of G protein alpha subunits in the main olfactory and vomeronasal systems of Rhinella (Bufo) arenarum tadpoles.
topic_facet guanine nucleotide binding protein alpha subunit
animal
article
larva
metabolism
olfactory system
toad
vomeronasal organ
Animals
Bufonidae
GTP-Binding Protein alpha Subunits
Larva
Olfactory Pathways
Vomeronasal Organ
description We evaluated the presence of G protein subtypes Galpha(o), Galpha(i2), and Galpha(olf) in the main olfactory system (MOS) and accessory or vomeronasal system (VNS) of Rhinella (Bufo) arenarum tadpoles, and here describe the fine structure of the sensory cells in the olfactory epithelium (OE) and vomeronasal organ (VNO). The OE shows olfactory receptor neurons (ORNs) with cilia in the apical surface, and the vomeronasal receptor neurons (VRNs) of the VNO are covered with microvilli. Immunohistochemistry detected the presence of at least two segregated populations of ORNs throughout the OE, coupled to Galpha(olf) and Galpha(o). An antiserum against Galpha(i2) was ineffective in staining the ORNs. In the VNO, Galpha(o) neurons stained strongly but lacked immunoreactivity to any other Galpha subunit in all larval stages analyzed. Western blot analyses and preabsorption experiments confirmed the specificity of the commercial antisera used. The functional significance of the heterogeneous G-protein distribution in R. arenarum tadpoles is not clear, but the study of G- protein distributions in various amphibian species is important, since this vertebrate group played a key role in the evolution of tetrapods. A more complete knowledge of the amphibian MOS and VNS would help to understand the functional organization and evolution of vertebrate chemosensory systems. This work demonstrates, for the first time, the existence of a segregated distribution of G-proteins in the OE of R. arenarum tadpoles.
format JOUR
author Jungblut, L.D.
Paz, D.A.
López-Costa, J.J.
Pozzi, A.G.
author_facet Jungblut, L.D.
Paz, D.A.
López-Costa, J.J.
Pozzi, A.G.
author_sort Jungblut, L.D.
title Heterogeneous distribution of G protein alpha subunits in the main olfactory and vomeronasal systems of Rhinella (Bufo) arenarum tadpoles.
title_short Heterogeneous distribution of G protein alpha subunits in the main olfactory and vomeronasal systems of Rhinella (Bufo) arenarum tadpoles.
title_full Heterogeneous distribution of G protein alpha subunits in the main olfactory and vomeronasal systems of Rhinella (Bufo) arenarum tadpoles.
title_fullStr Heterogeneous distribution of G protein alpha subunits in the main olfactory and vomeronasal systems of Rhinella (Bufo) arenarum tadpoles.
title_full_unstemmed Heterogeneous distribution of G protein alpha subunits in the main olfactory and vomeronasal systems of Rhinella (Bufo) arenarum tadpoles.
title_sort heterogeneous distribution of g protein alpha subunits in the main olfactory and vomeronasal systems of rhinella (bufo) arenarum tadpoles.
url http://hdl.handle.net/20.500.12110/paper_02890003_v26_n10_p722_Jungblut
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