Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus
A galactofuranosyl-containing glycopeptide has been isolated from mycelium of Ascobolus furfuraceus by extraction with water. The glycoconjugate was purified by DEAE-cellulose chromatography followed by gel filtration. A molecular weight of about 20 000 was determined by the latter method using stan...
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todo:paper_02820080_v3_n1_p45_Groisman2023-10-03T15:17:14Z Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus Groisman, J.F. Mercuri, O.A. De Lederkremer, R.M. Ascobolus furfuraceus galactofuranosyl-containing glycopeptide glycopeptide beta galactofuranose fungus nonhuman A galactofuranosyl-containing glycopeptide has been isolated from mycelium of Ascobolus furfuraceus by extraction with water. The glycoconjugate was purified by DEAE-cellulose chromatography followed by gel filtration. A molecular weight of about 20 000 was determined by the latter method using standard dextrans. Neutral sugars accounted for 94.5% of the glycopeptide and were characterized as mannose, galactose, and glucose. Glucosamine was estimated colorimetrically (1.8%). The molar ratio of Man:Gal:Glc:GlcNH2 was 68:32:16:2. A trace amount of total phosphorus (0.2%) was found. The predominant amino acids were threonine and serine. The peptide moiety was labeled with [14C]formaldehyde and the elution of radioactivity was coincident with sugar on gel filtration in the presence of sodium dodecyl sulfate. The peak of radioactivity was retarded on release of galactose by mild acid hydrolysis. These results confirm the sugar-peptide linkage. © 1986 Glycoconjugate Journal. Fil:Groisman, J.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mercuri, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02820080_v3_n1_p45_Groisman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Ascobolus furfuraceus galactofuranosyl-containing glycopeptide glycopeptide beta galactofuranose fungus nonhuman |
| spellingShingle |
Ascobolus furfuraceus galactofuranosyl-containing glycopeptide glycopeptide beta galactofuranose fungus nonhuman Groisman, J.F. Mercuri, O.A. De Lederkremer, R.M. Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus |
| topic_facet |
Ascobolus furfuraceus galactofuranosyl-containing glycopeptide glycopeptide beta galactofuranose fungus nonhuman |
| description |
A galactofuranosyl-containing glycopeptide has been isolated from mycelium of Ascobolus furfuraceus by extraction with water. The glycoconjugate was purified by DEAE-cellulose chromatography followed by gel filtration. A molecular weight of about 20 000 was determined by the latter method using standard dextrans. Neutral sugars accounted for 94.5% of the glycopeptide and were characterized as mannose, galactose, and glucose. Glucosamine was estimated colorimetrically (1.8%). The molar ratio of Man:Gal:Glc:GlcNH2 was 68:32:16:2. A trace amount of total phosphorus (0.2%) was found. The predominant amino acids were threonine and serine. The peptide moiety was labeled with [14C]formaldehyde and the elution of radioactivity was coincident with sugar on gel filtration in the presence of sodium dodecyl sulfate. The peak of radioactivity was retarded on release of galactose by mild acid hydrolysis. These results confirm the sugar-peptide linkage. © 1986 Glycoconjugate Journal. |
| format |
JOUR |
| author |
Groisman, J.F. Mercuri, O.A. De Lederkremer, R.M. |
| author_facet |
Groisman, J.F. Mercuri, O.A. De Lederkremer, R.M. |
| author_sort |
Groisman, J.F. |
| title |
Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus |
| title_short |
Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus |
| title_full |
Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus |
| title_fullStr |
Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus |
| title_full_unstemmed |
Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus |
| title_sort |
isolation and structural studies on a galactofuranosyl-containing glycopeptide from ascobolus furfuraceus |
| url |
http://hdl.handle.net/20.500.12110/paper_02820080_v3_n1_p45_Groisman |
| work_keys_str_mv |
AT groismanjf isolationandstructuralstudiesonagalactofuranosylcontainingglycopeptidefromascobolusfurfuraceus AT mercurioa isolationandstructuralstudiesonagalactofuranosylcontainingglycopeptidefromascobolusfurfuraceus AT delederkremerrm isolationandstructuralstudiesonagalactofuranosylcontainingglycopeptidefromascobolusfurfuraceus |
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1807324184729092096 |