A "synaptoplasmic cistern" mediates rapid inhibition of cochlear hair cells
Cochlear hair cells are inhibited by cholinergic efferent neurons. The acetylcholine (ACh) receptor of the hair cell is a ligand-gated cation channel through which calcium enters to activate potassium channels and hyperpolarize the cell. It has been proposed that calcium-induced calcium release (CIC...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_02706474_v24_n49_p11160_Lioudyno |
| Aporte de: |
| id |
todo:paper_02706474_v24_n49_p11160_Lioudyno |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_02706474_v24_n49_p11160_Lioudyno2023-10-03T15:14:38Z A "synaptoplasmic cistern" mediates rapid inhibition of cochlear hair cells Lioudyno, M. Hiel, H. Kong, J.-H. Katz, E. Waldman, E. Parameshwaran-Iyer, S. Glowatzki, E. Fuchs, P.A. Acetylcholine Calcium Cochlea Hair cell Inhibition Nicotinic acetylcholine ryanodine receptor ryanodine receptor 1 unclassified drug amplitude modulation animal experiment article cochlea controlled study hair cell nonhuman priority journal protein expression rat sarcoplasmic reticulum synaptic membrane Acetylcholine Animals Calcium Electrophysiology Endoplasmic Reticulum Evoked Potentials Hair Cells Neural Inhibition Patch-Clamp Techniques Potassium Rats Rats, Sprague-Dawley Receptors, Nicotinic Ryanodine Receptor Calcium Release Channel Synapses Cochlear hair cells are inhibited by cholinergic efferent neurons. The acetylcholine (ACh) receptor of the hair cell is a ligand-gated cation channel through which calcium enters to activate potassium channels and hyperpolarize the cell. It has been proposed that calcium-induced calcium release (CICR) from a near-membrane postsynaptic store supplements this process. Here, we demonstrate expression of type I ryanodine receptors in outer hair cells in the apical turn of the rat cochlea. Consistent with this finding, ryanodine and other store-active compounds alter the amplitude of transient currents produced by synaptic release of ACh, as well as the response of the hair cell to exogenous ACh. Like the sarcoplasmic reticulum of muscle, the "synaptoplasmic" cistern of the hair cell efficiently couples synaptic input to CICR. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02706474_v24_n49_p11160_Lioudyno |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acetylcholine Calcium Cochlea Hair cell Inhibition Nicotinic acetylcholine ryanodine receptor ryanodine receptor 1 unclassified drug amplitude modulation animal experiment article cochlea controlled study hair cell nonhuman priority journal protein expression rat sarcoplasmic reticulum synaptic membrane Acetylcholine Animals Calcium Electrophysiology Endoplasmic Reticulum Evoked Potentials Hair Cells Neural Inhibition Patch-Clamp Techniques Potassium Rats Rats, Sprague-Dawley Receptors, Nicotinic Ryanodine Receptor Calcium Release Channel Synapses |
| spellingShingle |
Acetylcholine Calcium Cochlea Hair cell Inhibition Nicotinic acetylcholine ryanodine receptor ryanodine receptor 1 unclassified drug amplitude modulation animal experiment article cochlea controlled study hair cell nonhuman priority journal protein expression rat sarcoplasmic reticulum synaptic membrane Acetylcholine Animals Calcium Electrophysiology Endoplasmic Reticulum Evoked Potentials Hair Cells Neural Inhibition Patch-Clamp Techniques Potassium Rats Rats, Sprague-Dawley Receptors, Nicotinic Ryanodine Receptor Calcium Release Channel Synapses Lioudyno, M. Hiel, H. Kong, J.-H. Katz, E. Waldman, E. Parameshwaran-Iyer, S. Glowatzki, E. Fuchs, P.A. A "synaptoplasmic cistern" mediates rapid inhibition of cochlear hair cells |
| topic_facet |
Acetylcholine Calcium Cochlea Hair cell Inhibition Nicotinic acetylcholine ryanodine receptor ryanodine receptor 1 unclassified drug amplitude modulation animal experiment article cochlea controlled study hair cell nonhuman priority journal protein expression rat sarcoplasmic reticulum synaptic membrane Acetylcholine Animals Calcium Electrophysiology Endoplasmic Reticulum Evoked Potentials Hair Cells Neural Inhibition Patch-Clamp Techniques Potassium Rats Rats, Sprague-Dawley Receptors, Nicotinic Ryanodine Receptor Calcium Release Channel Synapses |
| description |
Cochlear hair cells are inhibited by cholinergic efferent neurons. The acetylcholine (ACh) receptor of the hair cell is a ligand-gated cation channel through which calcium enters to activate potassium channels and hyperpolarize the cell. It has been proposed that calcium-induced calcium release (CICR) from a near-membrane postsynaptic store supplements this process. Here, we demonstrate expression of type I ryanodine receptors in outer hair cells in the apical turn of the rat cochlea. Consistent with this finding, ryanodine and other store-active compounds alter the amplitude of transient currents produced by synaptic release of ACh, as well as the response of the hair cell to exogenous ACh. Like the sarcoplasmic reticulum of muscle, the "synaptoplasmic" cistern of the hair cell efficiently couples synaptic input to CICR. |
| format |
JOUR |
| author |
Lioudyno, M. Hiel, H. Kong, J.-H. Katz, E. Waldman, E. Parameshwaran-Iyer, S. Glowatzki, E. Fuchs, P.A. |
| author_facet |
Lioudyno, M. Hiel, H. Kong, J.-H. Katz, E. Waldman, E. Parameshwaran-Iyer, S. Glowatzki, E. Fuchs, P.A. |
| author_sort |
Lioudyno, M. |
| title |
A "synaptoplasmic cistern" mediates rapid inhibition of cochlear hair cells |
| title_short |
A "synaptoplasmic cistern" mediates rapid inhibition of cochlear hair cells |
| title_full |
A "synaptoplasmic cistern" mediates rapid inhibition of cochlear hair cells |
| title_fullStr |
A "synaptoplasmic cistern" mediates rapid inhibition of cochlear hair cells |
| title_full_unstemmed |
A "synaptoplasmic cistern" mediates rapid inhibition of cochlear hair cells |
| title_sort |
"synaptoplasmic cistern" mediates rapid inhibition of cochlear hair cells |
| url |
http://hdl.handle.net/20.500.12110/paper_02706474_v24_n49_p11160_Lioudyno |
| work_keys_str_mv |
AT lioudynom asynaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT hielh asynaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT kongjh asynaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT katze asynaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT waldmane asynaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT parameshwaraniyers asynaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT glowatzkie asynaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT fuchspa asynaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT lioudynom synaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT hielh synaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT kongjh synaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT katze synaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT waldmane synaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT parameshwaraniyers synaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT glowatzkie synaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells AT fuchspa synaptoplasmiccisternmediatesrapidinhibitionofcochlearhaircells |
| _version_ |
1807318605576011776 |