Functional compensation of P/Q by N-type channels blocks short-term plasticity at the calyx of Held presynaptic terminal

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Calcium channels of the P/Q subtype mediate transmitter release at the neuromuscular junction and at many central synapses, such as the calyx of Held. Transgenic mice in which α1A channels are ablated provide a powerful tool with which to test compensatory mechanisms at the synapse and to explore me...

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Autores principales:	González Inchauspe, C., Martini, F.J., Forsythe, I.D., Uchitel, O.D.
Formato:	JOUR
Materias:	Calcium currents Calyx of Held Facilitation Knock-out mice P/Q channels Synaptic transmission calcium channel N type calcium channel P type calcium channel Q type calcium ion animal tissue article calcium current channel gating facilitation knockout mouse mouse nerve cell plasticity nonhuman presynaptic membrane priority journal synaptosome transgenic mouse Animals Brain Stem Calcium Calcium Channels, N-Type Calcium Channels, P-Type Calcium Channels, Q-Type Evoked Potentials Mice Mice, Knockout Neuronal Plasticity Presynaptic Terminals Protein Subunits Synapses Synaptic Transmission
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_02706474_v24_n46_p10379_GonzalezInchauspe
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_02706474_v24_n46_p10379_GonzalezInchauspe
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spelling	todo:paper_02706474_v24_n46_p10379_GonzalezInchauspe2023-10-03T15:14:37Z Functional compensation of P/Q by N-type channels blocks short-term plasticity at the calyx of Held presynaptic terminal González Inchauspe, C. Martini, F.J. Forsythe, I.D. Uchitel, O.D. Calcium currents Calyx of Held Facilitation Knock-out mice P/Q channels Synaptic transmission calcium channel N type calcium channel P type calcium channel Q type calcium ion animal tissue article calcium current channel gating facilitation knockout mouse mouse nerve cell plasticity nonhuman presynaptic membrane priority journal synaptosome transgenic mouse Animals Brain Stem Calcium Calcium Channels, N-Type Calcium Channels, P-Type Calcium Channels, Q-Type Evoked Potentials Mice Mice, Knockout Neuronal Plasticity Presynaptic Terminals Protein Subunits Synapses Synaptic Transmission Calcium channels of the P/Q subtype mediate transmitter release at the neuromuscular junction and at many central synapses, such as the calyx of Held. Transgenic mice in which α1A channels are ablated provide a powerful tool with which to test compensatory mechanisms at the synapse and to explore mechanisms of presynaptic regulation associated with expression of P/Q channels. Using the calyx of Held preparation from the knock-out (KO) mice, we show here that N-type channels functionally compensate for the absence of P/Q subunits at the calyx and evoke giant synaptic currents [approximately two-thirds of the magnitude of wild-type (WT) responses]. However, although evoked paired-pulse facilitation is prominent in WT, this facilitation is greatly diminished in the KO. In addition, direct recording of presynaptic calcium currents revealed that the major functional difference was the absence of calcium-dependent facilitation at the calyx in the P/Q KO animals. We conclude that one physiological function of P/Q channels is to provide additional facilitatory drive, so contributing to maintenance of transmission as vesicles are depleted during high throughput synaptic transmission. Fil:González Inchauspe, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Uchitel, O.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02706474_v24_n46_p10379_GonzalezInchauspe
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Calcium currents Calyx of Held Facilitation Knock-out mice P/Q channels Synaptic transmission calcium channel N type calcium channel P type calcium channel Q type calcium ion animal tissue article calcium current channel gating facilitation knockout mouse mouse nerve cell plasticity nonhuman presynaptic membrane priority journal synaptosome transgenic mouse Animals Brain Stem Calcium Calcium Channels, N-Type Calcium Channels, P-Type Calcium Channels, Q-Type Evoked Potentials Mice Mice, Knockout Neuronal Plasticity Presynaptic Terminals Protein Subunits Synapses Synaptic Transmission
spellingShingle	Calcium currents Calyx of Held Facilitation Knock-out mice P/Q channels Synaptic transmission calcium channel N type calcium channel P type calcium channel Q type calcium ion animal tissue article calcium current channel gating facilitation knockout mouse mouse nerve cell plasticity nonhuman presynaptic membrane priority journal synaptosome transgenic mouse Animals Brain Stem Calcium Calcium Channels, N-Type Calcium Channels, P-Type Calcium Channels, Q-Type Evoked Potentials Mice Mice, Knockout Neuronal Plasticity Presynaptic Terminals Protein Subunits Synapses Synaptic Transmission González Inchauspe, C. Martini, F.J. Forsythe, I.D. Uchitel, O.D. Functional compensation of P/Q by N-type channels blocks short-term plasticity at the calyx of Held presynaptic terminal
topic_facet	Calcium currents Calyx of Held Facilitation Knock-out mice P/Q channels Synaptic transmission calcium channel N type calcium channel P type calcium channel Q type calcium ion animal tissue article calcium current channel gating facilitation knockout mouse mouse nerve cell plasticity nonhuman presynaptic membrane priority journal synaptosome transgenic mouse Animals Brain Stem Calcium Calcium Channels, N-Type Calcium Channels, P-Type Calcium Channels, Q-Type Evoked Potentials Mice Mice, Knockout Neuronal Plasticity Presynaptic Terminals Protein Subunits Synapses Synaptic Transmission
description	Calcium channels of the P/Q subtype mediate transmitter release at the neuromuscular junction and at many central synapses, such as the calyx of Held. Transgenic mice in which α1A channels are ablated provide a powerful tool with which to test compensatory mechanisms at the synapse and to explore mechanisms of presynaptic regulation associated with expression of P/Q channels. Using the calyx of Held preparation from the knock-out (KO) mice, we show here that N-type channels functionally compensate for the absence of P/Q subunits at the calyx and evoke giant synaptic currents [approximately two-thirds of the magnitude of wild-type (WT) responses]. However, although evoked paired-pulse facilitation is prominent in WT, this facilitation is greatly diminished in the KO. In addition, direct recording of presynaptic calcium currents revealed that the major functional difference was the absence of calcium-dependent facilitation at the calyx in the P/Q KO animals. We conclude that one physiological function of P/Q channels is to provide additional facilitatory drive, so contributing to maintenance of transmission as vesicles are depleted during high throughput synaptic transmission.
format	JOUR
author	González Inchauspe, C. Martini, F.J. Forsythe, I.D. Uchitel, O.D.
author_facet	González Inchauspe, C. Martini, F.J. Forsythe, I.D. Uchitel, O.D.
author_sort	González Inchauspe, C.
title	Functional compensation of P/Q by N-type channels blocks short-term plasticity at the calyx of Held presynaptic terminal
title_short	Functional compensation of P/Q by N-type channels blocks short-term plasticity at the calyx of Held presynaptic terminal
title_full	Functional compensation of P/Q by N-type channels blocks short-term plasticity at the calyx of Held presynaptic terminal
title_fullStr	Functional compensation of P/Q by N-type channels blocks short-term plasticity at the calyx of Held presynaptic terminal
title_full_unstemmed	Functional compensation of P/Q by N-type channels blocks short-term plasticity at the calyx of Held presynaptic terminal
title_sort	functional compensation of p/q by n-type channels blocks short-term plasticity at the calyx of held presynaptic terminal
url	http://hdl.handle.net/20.500.12110/paper_02706474_v24_n46_p10379_GonzalezInchauspe
work_keys_str_mv	AT gonzalezinchauspec functionalcompensationofpqbyntypechannelsblocksshorttermplasticityatthecalyxofheldpresynapticterminal AT martinifj functionalcompensationofpqbyntypechannelsblocksshorttermplasticityatthecalyxofheldpresynapticterminal AT forsytheid functionalcompensationofpqbyntypechannelsblocksshorttermplasticityatthecalyxofheldpresynapticterminal AT uchitelod functionalcompensationofpqbyntypechannelsblocksshorttermplasticityatthecalyxofheldpresynapticterminal
_version_	1807316553363881984

Functional compensation of P/Q by N-type channels blocks short-term plasticity at the calyx of Held presynaptic terminal

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