Effect of chromium on the fatty acid composition of two strains of Euglena gracilis

The effect of hexavalent chromium on fatty acid composition was studied in two strains of Euglena gracilis; UTEX 753 (from the Culture Collection of Algae of Texas University, USA) and MAT (isolated from a highly polluted River). Both were grown in photoauxotrophic and photoheterotrophic conditions...

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Autores principales:	Rocchetta, I., Mazzuca, M., Conforti, V., Ruiz, L., Balzaretti, V., De Molina, M.D.C.R.
Formato:	JOUR
Materias:	Euglena gracilis Fatty acids Heavy metal Hexavalent chromium Lipids Malondialdehyde MAT Photoheterotrophic Algae Cells Chlorophyll Chromium Heavy metals Metals Oxidation Peroxides Photosynthesis Water pollution Microorganisms chromium polyunsaturated fatty acid fatty acid article auxotrophy chloroplast controlled study heterotrophy IC 50 lipid analysis lipid composition lipid peroxidation nonhuman thylakoid transmission electron microscopy ultrastructure Animals Carcinogens, Environmental Chloroplasts Culture Media Fatty Acids Microscopy, Electron Rivers Water Pollutants, Chemical algae
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_02697491_v141_n2_p353_Rocchetta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_02697491_v141_n2_p353_Rocchetta
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spelling	todo:paper_02697491_v141_n2_p353_Rocchetta2023-10-03T15:14:14Z Effect of chromium on the fatty acid composition of two strains of Euglena gracilis Rocchetta, I. Mazzuca, M. Conforti, V. Ruiz, L. Balzaretti, V. De Molina, M.D.C.R. Euglena gracilis Fatty acids Heavy metal Hexavalent chromium Lipids Euglena gracilis Hexavalent chromium Malondialdehyde MAT Photoheterotrophic Algae Cells Chlorophyll Chromium Fatty acids Heavy metals Lipids Metals Oxidation Peroxides Photosynthesis Water pollution Microorganisms chromium polyunsaturated fatty acid chromium fatty acid article auxotrophy chloroplast controlled study Euglena gracilis heterotrophy IC 50 lipid analysis lipid composition lipid peroxidation nonhuman thylakoid transmission electron microscopy ultrastructure Animals Carcinogens, Environmental Chloroplasts Chromium Culture Media Euglena gracilis Fatty Acids Lipids Malondialdehyde Microscopy, Electron Rivers Water Pollutants, Chemical algae Euglena gracilis The effect of hexavalent chromium on fatty acid composition was studied in two strains of Euglena gracilis; UTEX 753 (from the Culture Collection of Algae of Texas University, USA) and MAT (isolated from a highly polluted River). Both were grown in photoauxotrophic and photoheterotrophic conditions and exposed to two metal concentrations, one below and one above IC50. The high malondialdehyde (MDA) levels (3 to 7-fold) obtained with chromium concentration above IC50, suggested the existence of metal-induced lipid peroxidation. Total lipid content increased only with concentration below IC50, whereas it was inhibited by higher metal concentration. Photoheterotrophic control strains exhibited a significantly higher proportion of saturated and polyunsaturated fatty acids. Polyunsaturated acids were most affected by chromium, especially those related to chloroplast structures. Ultra-structure studies showed clear thylakoid disorganization in all treated cells. The results indicate that hexavalent chromium affects levels of fatty acids, especially those related to photosynthetic activity. © 2005 Elsevier Ltd. All rights reserved. Fil:Rocchetta, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Conforti, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruiz, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02697491_v141_n2_p353_Rocchetta
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Euglena gracilis Fatty acids Heavy metal Hexavalent chromium Lipids Euglena gracilis Hexavalent chromium Malondialdehyde MAT Photoheterotrophic Algae Cells Chlorophyll Chromium Fatty acids Heavy metals Lipids Metals Oxidation Peroxides Photosynthesis Water pollution Microorganisms chromium polyunsaturated fatty acid chromium fatty acid article auxotrophy chloroplast controlled study Euglena gracilis heterotrophy IC 50 lipid analysis lipid composition lipid peroxidation nonhuman thylakoid transmission electron microscopy ultrastructure Animals Carcinogens, Environmental Chloroplasts Chromium Culture Media Euglena gracilis Fatty Acids Lipids Malondialdehyde Microscopy, Electron Rivers Water Pollutants, Chemical algae Euglena gracilis
spellingShingle	Euglena gracilis Fatty acids Heavy metal Hexavalent chromium Lipids Euglena gracilis Hexavalent chromium Malondialdehyde MAT Photoheterotrophic Algae Cells Chlorophyll Chromium Fatty acids Heavy metals Lipids Metals Oxidation Peroxides Photosynthesis Water pollution Microorganisms chromium polyunsaturated fatty acid chromium fatty acid article auxotrophy chloroplast controlled study Euglena gracilis heterotrophy IC 50 lipid analysis lipid composition lipid peroxidation nonhuman thylakoid transmission electron microscopy ultrastructure Animals Carcinogens, Environmental Chloroplasts Chromium Culture Media Euglena gracilis Fatty Acids Lipids Malondialdehyde Microscopy, Electron Rivers Water Pollutants, Chemical algae Euglena gracilis Rocchetta, I. Mazzuca, M. Conforti, V. Ruiz, L. Balzaretti, V. De Molina, M.D.C.R. Effect of chromium on the fatty acid composition of two strains of Euglena gracilis
topic_facet	Euglena gracilis Fatty acids Heavy metal Hexavalent chromium Lipids Euglena gracilis Hexavalent chromium Malondialdehyde MAT Photoheterotrophic Algae Cells Chlorophyll Chromium Fatty acids Heavy metals Lipids Metals Oxidation Peroxides Photosynthesis Water pollution Microorganisms chromium polyunsaturated fatty acid chromium fatty acid article auxotrophy chloroplast controlled study Euglena gracilis heterotrophy IC 50 lipid analysis lipid composition lipid peroxidation nonhuman thylakoid transmission electron microscopy ultrastructure Animals Carcinogens, Environmental Chloroplasts Chromium Culture Media Euglena gracilis Fatty Acids Lipids Malondialdehyde Microscopy, Electron Rivers Water Pollutants, Chemical algae Euglena gracilis
description	The effect of hexavalent chromium on fatty acid composition was studied in two strains of Euglena gracilis; UTEX 753 (from the Culture Collection of Algae of Texas University, USA) and MAT (isolated from a highly polluted River). Both were grown in photoauxotrophic and photoheterotrophic conditions and exposed to two metal concentrations, one below and one above IC50. The high malondialdehyde (MDA) levels (3 to 7-fold) obtained with chromium concentration above IC50, suggested the existence of metal-induced lipid peroxidation. Total lipid content increased only with concentration below IC50, whereas it was inhibited by higher metal concentration. Photoheterotrophic control strains exhibited a significantly higher proportion of saturated and polyunsaturated fatty acids. Polyunsaturated acids were most affected by chromium, especially those related to chloroplast structures. Ultra-structure studies showed clear thylakoid disorganization in all treated cells. The results indicate that hexavalent chromium affects levels of fatty acids, especially those related to photosynthetic activity. © 2005 Elsevier Ltd. All rights reserved.
format	JOUR
author	Rocchetta, I. Mazzuca, M. Conforti, V. Ruiz, L. Balzaretti, V. De Molina, M.D.C.R.
author_facet	Rocchetta, I. Mazzuca, M. Conforti, V. Ruiz, L. Balzaretti, V. De Molina, M.D.C.R.
author_sort	Rocchetta, I.
title	Effect of chromium on the fatty acid composition of two strains of Euglena gracilis
title_short	Effect of chromium on the fatty acid composition of two strains of Euglena gracilis
title_full	Effect of chromium on the fatty acid composition of two strains of Euglena gracilis
title_fullStr	Effect of chromium on the fatty acid composition of two strains of Euglena gracilis
title_full_unstemmed	Effect of chromium on the fatty acid composition of two strains of Euglena gracilis
title_sort	effect of chromium on the fatty acid composition of two strains of euglena gracilis
url	http://hdl.handle.net/20.500.12110/paper_02697491_v141_n2_p353_Rocchetta
work_keys_str_mv	AT rocchettai effectofchromiumonthefattyacidcompositionoftwostrainsofeuglenagracilis AT mazzucam effectofchromiumonthefattyacidcompositionoftwostrainsofeuglenagracilis AT confortiv effectofchromiumonthefattyacidcompositionoftwostrainsofeuglenagracilis AT ruizl effectofchromiumonthefattyacidcompositionoftwostrainsofeuglenagracilis AT balzarettiv effectofchromiumonthefattyacidcompositionoftwostrainsofeuglenagracilis AT demolinamdcr effectofchromiumonthefattyacidcompositionoftwostrainsofeuglenagracilis
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Effect of chromium on the fatty acid composition of two strains of Euglena gracilis

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