Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo

Background: Human sperm decondense in vitro upon exposure to heparin and glutathione. The aim of this study was to evaluate whether this decondensing ability of heparin in vitro is related to structural characteristics of the molecule and to test the in-vitro decondensing ability of other glycosamin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Romanato, M., Cameo, M.S., Bertolesi, G., Baldini, C., Calvo, J.C., Calvo, L.
Formato:	JOUR
Materias:	Fertilization Gamete biology Sperm dermatan sulfate glutaraldehyde glutathione glycosaminoglycan heparan sulfate heparin heparin derivative hyaluronic acid article controlled study dose response drug activity drug effect drug sulfation human human cell in vitro study in vivo study male molecular weight normal human phase contrast microscopy sperm spermatozoon structure activity relation
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_02681161_v18_n9_p1868_Romanato
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_02681161_v18_n9_p1868_Romanato
record_format	dspace
spelling	todo:paper_02681161_v18_n9_p1868_Romanato2023-10-03T15:13:55Z Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo Romanato, M. Cameo, M.S. Bertolesi, G. Baldini, C. Calvo, J.C. Calvo, L. Fertilization Gamete biology Sperm dermatan sulfate glutaraldehyde glutathione glycosaminoglycan heparan sulfate heparin heparin derivative hyaluronic acid article controlled study dose response drug activity drug effect drug sulfation human human cell in vitro study in vivo study male molecular weight normal human phase contrast microscopy sperm spermatozoon structure activity relation Background: Human sperm decondense in vitro upon exposure to heparin and glutathione. The aim of this study was to evaluate whether this decondensing ability of heparin in vitro is related to structural characteristics of the molecule and to test the in-vitro decondensing ability of other glycosaminoglycans. Methods: Capacitated sperm obtained from normospermic semen samples were decondensed in the presence of heparin (or its equivalent) and glutathione. After fixation with glutaraldehyde, the percentage of decondensed sperm was determined under phase contrast. Results: The decondensing ability of heparin was related to sulphation characteristics of the molecule: heparin, O-desulphated heparin and N-desulphated-N-acetylated heparin had similar decondensing abilities; N-desulphated was less active and O/N-desulphated-N-acetylated heparin was completely inactive. On the other hand, the decondensing ability of heparin was not affected by molecular weight, within the range 3000-18 000 kDa. When the decondensing ability of different glycosaminoglycans was tested, heparin and heparin sulphate were equally active, while chondroitin sulphate and hyaluronic acid were completely inactive and dermatan sulphate was slightly active. Conclusions: Our results indicate that heparin's decondensing ability in vitro is related to sulphation characteristics of the molecule and suggest that heparan sulphate, a structural analogue of heparin, could be a sperm-decondensing agent in vivo. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02681161_v18_n9_p1868_Romanato
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Fertilization Gamete biology Sperm dermatan sulfate glutaraldehyde glutathione glycosaminoglycan heparan sulfate heparin heparin derivative hyaluronic acid article controlled study dose response drug activity drug effect drug sulfation human human cell in vitro study in vivo study male molecular weight normal human phase contrast microscopy sperm spermatozoon structure activity relation
spellingShingle	Fertilization Gamete biology Sperm dermatan sulfate glutaraldehyde glutathione glycosaminoglycan heparan sulfate heparin heparin derivative hyaluronic acid article controlled study dose response drug activity drug effect drug sulfation human human cell in vitro study in vivo study male molecular weight normal human phase contrast microscopy sperm spermatozoon structure activity relation Romanato, M. Cameo, M.S. Bertolesi, G. Baldini, C. Calvo, J.C. Calvo, L. Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo
topic_facet	Fertilization Gamete biology Sperm dermatan sulfate glutaraldehyde glutathione glycosaminoglycan heparan sulfate heparin heparin derivative hyaluronic acid article controlled study dose response drug activity drug effect drug sulfation human human cell in vitro study in vivo study male molecular weight normal human phase contrast microscopy sperm spermatozoon structure activity relation
description	Background: Human sperm decondense in vitro upon exposure to heparin and glutathione. The aim of this study was to evaluate whether this decondensing ability of heparin in vitro is related to structural characteristics of the molecule and to test the in-vitro decondensing ability of other glycosaminoglycans. Methods: Capacitated sperm obtained from normospermic semen samples were decondensed in the presence of heparin (or its equivalent) and glutathione. After fixation with glutaraldehyde, the percentage of decondensed sperm was determined under phase contrast. Results: The decondensing ability of heparin was related to sulphation characteristics of the molecule: heparin, O-desulphated heparin and N-desulphated-N-acetylated heparin had similar decondensing abilities; N-desulphated was less active and O/N-desulphated-N-acetylated heparin was completely inactive. On the other hand, the decondensing ability of heparin was not affected by molecular weight, within the range 3000-18 000 kDa. When the decondensing ability of different glycosaminoglycans was tested, heparin and heparin sulphate were equally active, while chondroitin sulphate and hyaluronic acid were completely inactive and dermatan sulphate was slightly active. Conclusions: Our results indicate that heparin's decondensing ability in vitro is related to sulphation characteristics of the molecule and suggest that heparan sulphate, a structural analogue of heparin, could be a sperm-decondensing agent in vivo.
format	JOUR
author	Romanato, M. Cameo, M.S. Bertolesi, G. Baldini, C. Calvo, J.C. Calvo, L.
author_facet	Romanato, M. Cameo, M.S. Bertolesi, G. Baldini, C. Calvo, J.C. Calvo, L.
author_sort	Romanato, M.
title	Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo
title_short	Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo
title_full	Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo
title_fullStr	Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo
title_full_unstemmed	Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo
title_sort	heparan sulphate: a putative decondensing agent for human spermatozoa in vivo
url	http://hdl.handle.net/20.500.12110/paper_02681161_v18_n9_p1868_Romanato
work_keys_str_mv	AT romanatom heparansulphateaputativedecondensingagentforhumanspermatozoainvivo AT cameoms heparansulphateaputativedecondensingagentforhumanspermatozoainvivo AT bertolesig heparansulphateaputativedecondensingagentforhumanspermatozoainvivo AT baldinic heparansulphateaputativedecondensingagentforhumanspermatozoainvivo AT calvojc heparansulphateaputativedecondensingagentforhumanspermatozoainvivo AT calvol heparansulphateaputativedecondensingagentforhumanspermatozoainvivo
_version_	1782025216743964672

Heparan sulphate: A putative decondensing agent for human spermatozoa in vivo

Ejemplares similares