Metabolism of Klebsiella pneumoniae freeze-dried cultures for the design of BOD bioassays

Aims: The survival rate of freeze-dried cultures is not enough information for technological applications of micro-organisms. There could be serious metabolic/structural damage in the survivors, leading to a delay time that can jeopardize the design of a rapid biochemical oxygen demand (BOD) metabol...

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Autores principales:	Bonetto, M.C., Sacco, N.J., Hilding Ohlsson, A., Cortón, E.
Formato:	JOUR
Materias:	Amperometric assays Ferricyanide Freezedrying Glutamic acid solution OECDstd ferricyanide glutamate sodium ferrocyanide oxygen bioassay biochemical oxygen demand colony structure dry density metabolism microbial activity microbial community survival article bacterial metabolism bacterial survival bacterium culture colony forming unit electrochemical analysis freeze drying Klebsiella pneumoniae milk nonhuman survival rate animal chemistry growth, development and aging procedures Article electrochemistry Animals Biological Oxygen Demand Analysis Ferrocyanides Freeze Drying Milk Oxygen Sodium Glutamate
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_02668254_v55_n5_p370_Bonetto
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_02668254_v55_n5_p370_Bonetto
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spelling	todo:paper_02668254_v55_n5_p370_Bonetto2023-10-03T15:13:27Z Metabolism of Klebsiella pneumoniae freeze-dried cultures for the design of BOD bioassays Bonetto, M.C. Sacco, N.J. Hilding Ohlsson, A. Cortón, E. Amperometric assays Ferricyanide Freezedrying Glutamic acid solution OECDstd ferricyanide glutamate sodium ferrocyanide oxygen bioassay biochemical oxygen demand colony structure dry density metabolism microbial activity microbial community survival article bacterial metabolism bacterial survival bacterium culture bioassay biochemical oxygen demand colony forming unit electrochemical analysis freeze drying Klebsiella pneumoniae milk nonhuman survival rate animal biochemical oxygen demand chemistry freeze drying growth, development and aging Klebsiella pneumoniae metabolism procedures Article bacterial metabolism bioassay electrochemistry Klebsiella pneumoniae Klebsiella pneumoniae Animals Biological Oxygen Demand Analysis Ferrocyanides Freeze Drying Klebsiella pneumoniae Milk Oxygen Sodium Glutamate Aims: The survival rate of freeze-dried cultures is not enough information for technological applications of micro-organisms. There could be serious metabolic/structural damage in the survivors, leading to a delay time that can jeopardize the design of a rapid biochemical oxygen demand (BOD) metabolicbased bioassay. Therefore, we will study the metabolic activity (as ferricyanide reduction activity) and the survival rate (as colony-forming units, CFU) of different Klebsiella pneumoniae freeze-dried cultures looking for stable metabolic conditions after 35 days of storage. Method and Results: Here, we tried several simple freeze-drying processes of Kl. pneumoniae. Electrochemical measurements of ferrocyanide and survival rates obtained with the different freeze-dried cultures were used to choose the best freeze-drying process that leads to a rapid metabolic-based bioassay. Conclusions: The use of milk plus monosodium glutamate was the best choice to obtain a Kl. pneumoniae freeze-dried culture with metabolic stable conditions after storage at )20°C without the need of vacuum storage and ready to use after 20 min of rehydration. We also demonstrate that the viability and the metabolic activity are not always directly correlated. Significance and Impact of the Study: This study shows that the use of this Kl. pneumoniae freeze-dried culture is appropriate for the design of a rapid BOD bioassay. © 2012 The Authors. © 2012 The Society for Applied Microbiology. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02668254_v55_n5_p370_Bonetto
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Amperometric assays Ferricyanide Freezedrying Glutamic acid solution OECDstd ferricyanide glutamate sodium ferrocyanide oxygen bioassay biochemical oxygen demand colony structure dry density metabolism microbial activity microbial community survival article bacterial metabolism bacterial survival bacterium culture bioassay biochemical oxygen demand colony forming unit electrochemical analysis freeze drying Klebsiella pneumoniae milk nonhuman survival rate animal biochemical oxygen demand chemistry freeze drying growth, development and aging Klebsiella pneumoniae metabolism procedures Article bacterial metabolism bioassay electrochemistry Klebsiella pneumoniae Klebsiella pneumoniae Animals Biological Oxygen Demand Analysis Ferrocyanides Freeze Drying Klebsiella pneumoniae Milk Oxygen Sodium Glutamate
spellingShingle	Amperometric assays Ferricyanide Freezedrying Glutamic acid solution OECDstd ferricyanide glutamate sodium ferrocyanide oxygen bioassay biochemical oxygen demand colony structure dry density metabolism microbial activity microbial community survival article bacterial metabolism bacterial survival bacterium culture bioassay biochemical oxygen demand colony forming unit electrochemical analysis freeze drying Klebsiella pneumoniae milk nonhuman survival rate animal biochemical oxygen demand chemistry freeze drying growth, development and aging Klebsiella pneumoniae metabolism procedures Article bacterial metabolism bioassay electrochemistry Klebsiella pneumoniae Klebsiella pneumoniae Animals Biological Oxygen Demand Analysis Ferrocyanides Freeze Drying Klebsiella pneumoniae Milk Oxygen Sodium Glutamate Bonetto, M.C. Sacco, N.J. Hilding Ohlsson, A. Cortón, E. Metabolism of Klebsiella pneumoniae freeze-dried cultures for the design of BOD bioassays
topic_facet	Amperometric assays Ferricyanide Freezedrying Glutamic acid solution OECDstd ferricyanide glutamate sodium ferrocyanide oxygen bioassay biochemical oxygen demand colony structure dry density metabolism microbial activity microbial community survival article bacterial metabolism bacterial survival bacterium culture bioassay biochemical oxygen demand colony forming unit electrochemical analysis freeze drying Klebsiella pneumoniae milk nonhuman survival rate animal biochemical oxygen demand chemistry freeze drying growth, development and aging Klebsiella pneumoniae metabolism procedures Article bacterial metabolism bioassay electrochemistry Klebsiella pneumoniae Klebsiella pneumoniae Animals Biological Oxygen Demand Analysis Ferrocyanides Freeze Drying Klebsiella pneumoniae Milk Oxygen Sodium Glutamate
description	Aims: The survival rate of freeze-dried cultures is not enough information for technological applications of micro-organisms. There could be serious metabolic/structural damage in the survivors, leading to a delay time that can jeopardize the design of a rapid biochemical oxygen demand (BOD) metabolicbased bioassay. Therefore, we will study the metabolic activity (as ferricyanide reduction activity) and the survival rate (as colony-forming units, CFU) of different Klebsiella pneumoniae freeze-dried cultures looking for stable metabolic conditions after 35 days of storage. Method and Results: Here, we tried several simple freeze-drying processes of Kl. pneumoniae. Electrochemical measurements of ferrocyanide and survival rates obtained with the different freeze-dried cultures were used to choose the best freeze-drying process that leads to a rapid metabolic-based bioassay. Conclusions: The use of milk plus monosodium glutamate was the best choice to obtain a Kl. pneumoniae freeze-dried culture with metabolic stable conditions after storage at )20°C without the need of vacuum storage and ready to use after 20 min of rehydration. We also demonstrate that the viability and the metabolic activity are not always directly correlated. Significance and Impact of the Study: This study shows that the use of this Kl. pneumoniae freeze-dried culture is appropriate for the design of a rapid BOD bioassay. © 2012 The Authors. © 2012 The Society for Applied Microbiology.
format	JOUR
author	Bonetto, M.C. Sacco, N.J. Hilding Ohlsson, A. Cortón, E.
author_facet	Bonetto, M.C. Sacco, N.J. Hilding Ohlsson, A. Cortón, E.
author_sort	Bonetto, M.C.
title	Metabolism of Klebsiella pneumoniae freeze-dried cultures for the design of BOD bioassays
title_short	Metabolism of Klebsiella pneumoniae freeze-dried cultures for the design of BOD bioassays
title_full	Metabolism of Klebsiella pneumoniae freeze-dried cultures for the design of BOD bioassays
title_fullStr	Metabolism of Klebsiella pneumoniae freeze-dried cultures for the design of BOD bioassays
title_full_unstemmed	Metabolism of Klebsiella pneumoniae freeze-dried cultures for the design of BOD bioassays
title_sort	metabolism of klebsiella pneumoniae freeze-dried cultures for the design of bod bioassays
url	http://hdl.handle.net/20.500.12110/paper_02668254_v55_n5_p370_Bonetto
work_keys_str_mv	AT bonettomc metabolismofklebsiellapneumoniaefreezedriedculturesforthedesignofbodbioassays AT sacconj metabolismofklebsiellapneumoniaefreezedriedculturesforthedesignofbodbioassays AT hildingohlssona metabolismofklebsiellapneumoniaefreezedriedculturesforthedesignofbodbioassays AT cortone metabolismofklebsiellapneumoniaefreezedriedculturesforthedesignofbodbioassays
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Metabolism of Klebsiella pneumoniae freeze-dried cultures for the design of BOD bioassays

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