Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain
Aim: To maximize biomass production of an ochratoxigenic mould-controlling strain of Lachancea thermotolerans employing response surface methodology (RSM). Methods and Results: Using Plackett-Burman screening designs (PBSD) and central composite designs (CCD), an optimized culture medium containing...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | JOUR |
| Lenguaje: | English |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_02668254_v54_n5_p377_Pelinski |
| Aporte de: |
| id |
todo:paper_02668254_v54_n5_p377_Pelinski |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_02668254_v54_n5_p377_Pelinski2023-10-03T15:13:26Z Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain Pelinski, R. Cerrutti, P. Ponsone, M.L. Chulze, S. Galvagno, M. Biocontrol agent Cane molasses Experimental designs Fermentation optimization Lachancea thermotolerans molasses sugar biocontrol agent biomass bioreactor experimental design fermentation optimization sugar cane sustainability temperature tolerance yeast article batch fermentation biological pest control biomass bioreactor culture medium culture optimization dry weight fungal strain Lachancea thermotolerans mould nonhuman productivity response surface method sugarcane yeast Antibiosis Biological Control Agents Biomass Culture Media Fermentation Fungi Statistics as Topic Yeasts Lachancea Saccharum Aim: To maximize biomass production of an ochratoxigenic mould-controlling strain of Lachancea thermotolerans employing response surface methodology (RSM). Methods and Results: Using Plackett-Burman screening designs (PBSD) and central composite designs (CCD), an optimized culture medium containing (gl -1): fermentable sugars (FS), 139·2, provided by sugar cane molasses (CMz), (NH 4) 2HPO 4 (DAP), 9·0, and yeast extract (YE), 2·5, was formulated. Maximal cell concentration obtained after 24h at 28°C was 24·2gl -1cell dry weight (CDW). The mathematical model obtained was validated in experiments performed in shaken-flask cultures and also in aerated bioreactors. Maximum yield and productivity values achieved were, respectively, of 0·23g CDW/g FS in a medium containing (gl -1): FS, 87·0; DAP, 7·0; YE, 1·0; and of 0·96g CDW l -1 h -1 in a medium containing (gl -1): FS, 150·8 plus DAP, 6·9. Conclusions: Optimized culture conditions for maximizing yeast biomass production determined in flask cultures were applicable at a larger scale. The highest yield values were attained in media containing relatively low-CMz concentrations supplemented with DAP and YE. Yeast extract would not be necessary if higher productivity is the aim. Significance and Impact of the Study: Cells of L. thermotolerans produced aerobically could be sustainably produced in a medium just containing cheap carbon, nitrogen and phosphorus sources. Response surface methodology allowed the fine-tuning of cultural conditions. © 2012 The Authors. Letters in Applied Microbiology © 2012 The Society for Applied Microbiology. Fil:Cerrutti, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Galvagno, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02668254_v54_n5_p377_Pelinski |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
English |
| orig_language_str_mv |
English |
| topic |
Biocontrol agent Cane molasses Experimental designs Fermentation optimization Lachancea thermotolerans molasses sugar biocontrol agent biomass bioreactor experimental design fermentation optimization sugar cane sustainability temperature tolerance yeast article batch fermentation biological pest control biomass bioreactor culture medium culture optimization dry weight fungal strain Lachancea thermotolerans mould nonhuman productivity response surface method sugarcane yeast Antibiosis Biological Control Agents Biomass Culture Media Fermentation Fungi Statistics as Topic Yeasts Lachancea Saccharum |
| spellingShingle |
Biocontrol agent Cane molasses Experimental designs Fermentation optimization Lachancea thermotolerans molasses sugar biocontrol agent biomass bioreactor experimental design fermentation optimization sugar cane sustainability temperature tolerance yeast article batch fermentation biological pest control biomass bioreactor culture medium culture optimization dry weight fungal strain Lachancea thermotolerans mould nonhuman productivity response surface method sugarcane yeast Antibiosis Biological Control Agents Biomass Culture Media Fermentation Fungi Statistics as Topic Yeasts Lachancea Saccharum Pelinski, R. Cerrutti, P. Ponsone, M.L. Chulze, S. Galvagno, M. Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain |
| topic_facet |
Biocontrol agent Cane molasses Experimental designs Fermentation optimization Lachancea thermotolerans molasses sugar biocontrol agent biomass bioreactor experimental design fermentation optimization sugar cane sustainability temperature tolerance yeast article batch fermentation biological pest control biomass bioreactor culture medium culture optimization dry weight fungal strain Lachancea thermotolerans mould nonhuman productivity response surface method sugarcane yeast Antibiosis Biological Control Agents Biomass Culture Media Fermentation Fungi Statistics as Topic Yeasts Lachancea Saccharum |
| description |
Aim: To maximize biomass production of an ochratoxigenic mould-controlling strain of Lachancea thermotolerans employing response surface methodology (RSM). Methods and Results: Using Plackett-Burman screening designs (PBSD) and central composite designs (CCD), an optimized culture medium containing (gl -1): fermentable sugars (FS), 139·2, provided by sugar cane molasses (CMz), (NH 4) 2HPO 4 (DAP), 9·0, and yeast extract (YE), 2·5, was formulated. Maximal cell concentration obtained after 24h at 28°C was 24·2gl -1cell dry weight (CDW). The mathematical model obtained was validated in experiments performed in shaken-flask cultures and also in aerated bioreactors. Maximum yield and productivity values achieved were, respectively, of 0·23g CDW/g FS in a medium containing (gl -1): FS, 87·0; DAP, 7·0; YE, 1·0; and of 0·96g CDW l -1 h -1 in a medium containing (gl -1): FS, 150·8 plus DAP, 6·9. Conclusions: Optimized culture conditions for maximizing yeast biomass production determined in flask cultures were applicable at a larger scale. The highest yield values were attained in media containing relatively low-CMz concentrations supplemented with DAP and YE. Yeast extract would not be necessary if higher productivity is the aim. Significance and Impact of the Study: Cells of L. thermotolerans produced aerobically could be sustainably produced in a medium just containing cheap carbon, nitrogen and phosphorus sources. Response surface methodology allowed the fine-tuning of cultural conditions. © 2012 The Authors. Letters in Applied Microbiology © 2012 The Society for Applied Microbiology. |
| format |
JOUR |
| author |
Pelinski, R. Cerrutti, P. Ponsone, M.L. Chulze, S. Galvagno, M. |
| author_facet |
Pelinski, R. Cerrutti, P. Ponsone, M.L. Chulze, S. Galvagno, M. |
| author_sort |
Pelinski, R. |
| title |
Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain |
| title_short |
Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain |
| title_full |
Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain |
| title_fullStr |
Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain |
| title_full_unstemmed |
Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain |
| title_sort |
statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain |
| url |
http://hdl.handle.net/20.500.12110/paper_02668254_v54_n5_p377_Pelinski |
| work_keys_str_mv |
AT pelinskir statisticaloptimizationofsimplecultureconditionstoproducebiomassofanochratoxigenicmouldbiocontrolyeaststrain AT cerruttip statisticaloptimizationofsimplecultureconditionstoproducebiomassofanochratoxigenicmouldbiocontrolyeaststrain AT ponsoneml statisticaloptimizationofsimplecultureconditionstoproducebiomassofanochratoxigenicmouldbiocontrolyeaststrain AT chulzes statisticaloptimizationofsimplecultureconditionstoproducebiomassofanochratoxigenicmouldbiocontrolyeaststrain AT galvagnom statisticaloptimizationofsimplecultureconditionstoproducebiomassofanochratoxigenicmouldbiocontrolyeaststrain |
| _version_ |
1807324536469716992 |