Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis
Fusarium oxysporum f. sp. melonis produces cellulase and β-glucosidase activities in a medium with glucose and avicel as carbon source. A β-glucosidase from this crude material was purified by gel filtration and ion exchange chromatography successively. This enzyme is a unique band of protein in SDS...
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todo:paper_02668254_v22_n2_p106_Alconada2023-10-03T15:13:24Z Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis Alconada, T.M. Martínez, M.J. beta glucosidase article controlled study enzyme activity enzyme analysis enzyme purification Fusarium oxysporum nonhuman pH temperature Fungi Fusarium oxysporum f. sp. melonis Fusarium sp. Melonis Fusarium oxysporum f. sp. melonis produces cellulase and β-glucosidase activities in a medium with glucose and avicel as carbon source. A β-glucosidase from this crude material was purified by gel filtration and ion exchange chromatography successively. This enzyme is a unique band of protein in SDS-PAGE and isoelectric focussing. It had a molecular weight of 66,000 and a pI of 5. Using p-nitrophenyl-β-D-glucopyranoside as substrate β-glucosidase shows a K(m), of 210 μmol l -1 , an optimum pH of 5.5 and an optimum reaction temperature of 60°C, being stable in a pH range of 5-7 for 48 h at room temperature. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02668254_v22_n2_p106_Alconada |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
beta glucosidase article controlled study enzyme activity enzyme analysis enzyme purification Fusarium oxysporum nonhuman pH temperature Fungi Fusarium oxysporum f. sp. melonis Fusarium sp. Melonis |
| spellingShingle |
beta glucosidase article controlled study enzyme activity enzyme analysis enzyme purification Fusarium oxysporum nonhuman pH temperature Fungi Fusarium oxysporum f. sp. melonis Fusarium sp. Melonis Alconada, T.M. Martínez, M.J. Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis |
| topic_facet |
beta glucosidase article controlled study enzyme activity enzyme analysis enzyme purification Fusarium oxysporum nonhuman pH temperature Fungi Fusarium oxysporum f. sp. melonis Fusarium sp. Melonis |
| description |
Fusarium oxysporum f. sp. melonis produces cellulase and β-glucosidase activities in a medium with glucose and avicel as carbon source. A β-glucosidase from this crude material was purified by gel filtration and ion exchange chromatography successively. This enzyme is a unique band of protein in SDS-PAGE and isoelectric focussing. It had a molecular weight of 66,000 and a pI of 5. Using p-nitrophenyl-β-D-glucopyranoside as substrate β-glucosidase shows a K(m), of 210 μmol l -1 , an optimum pH of 5.5 and an optimum reaction temperature of 60°C, being stable in a pH range of 5-7 for 48 h at room temperature. |
| format |
JOUR |
| author |
Alconada, T.M. Martínez, M.J. |
| author_facet |
Alconada, T.M. Martínez, M.J. |
| author_sort |
Alconada, T.M. |
| title |
Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis |
| title_short |
Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis |
| title_full |
Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis |
| title_fullStr |
Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis |
| title_full_unstemmed |
Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis |
| title_sort |
purification and characterization of a β-glucosidase from the phytopathogenic fungus fusarium oxysporum f. sp. melonis |
| url |
http://hdl.handle.net/20.500.12110/paper_02668254_v22_n2_p106_Alconada |
| work_keys_str_mv |
AT alconadatm purificationandcharacterizationofabglucosidasefromthephytopathogenicfungusfusariumoxysporumfspmelonis AT martinezmj purificationandcharacterizationofabglucosidasefromthephytopathogenicfungusfusariumoxysporumfspmelonis |
| _version_ |
1807317773205897216 |