Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells

We examined the role of protein kinase Cα (PKCα) in the stimulation of DNA synthesis of Swiss 3T3 cells induced by bombesin, platelet-derived growth factor (PDGF) and phorbol 12-myristate 13-acetate (PMA). We found that cells in which this kinase had been down-regulated showed a partially abrogated...

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Autores principales:	Florin-Christensen, J., Florin-Christensen, M., Meinardi, E., Calle, R.
Formato:	JOUR
Materias:	2 [1 (3 amidinothiopropyl) 1h indol 3 yl] 3 (1 methyl 1h indol 3 yl)maleimide bombesin DNA phorbol 13 acetate 12 myristate platelet derived growth factor protein kinase C protein kinase C inhibitor animal cell article cell proliferation cell strain 3T3 controlled study DNA synthesis enzyme activation enzyme subunit mitogenesis mouse nonhuman priority journal protein transport Animalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_02646021_v315_n2_p513_FlorinChristensen
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_02646021_v315_n2_p513_FlorinChristensen
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spelling	todo:paper_02646021_v315_n2_p513_FlorinChristensen2023-10-03T15:12:57Z Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells Florin-Christensen, J. Florin-Christensen, M. Meinardi, E. Calle, R. 2 [1 (3 amidinothiopropyl) 1h indol 3 yl] 3 (1 methyl 1h indol 3 yl)maleimide bombesin DNA phorbol 13 acetate 12 myristate platelet derived growth factor protein kinase C protein kinase C inhibitor animal cell article cell proliferation cell strain 3T3 controlled study DNA synthesis enzyme activation enzyme subunit mitogenesis mouse nonhuman priority journal protein transport Animalia We examined the role of protein kinase Cα (PKCα) in the stimulation of DNA synthesis of Swiss 3T3 cells induced by bombesin, platelet-derived growth factor (PDGF) and phorbol 12-myristate 13-acetate (PMA). We found that cells in which this kinase had been down-regulated showed a partially abrogated mitogenic response to bombesin. The response to PDGF was unaltered; however, the response to PMA was completely suppressed. The mitogenic effect of maximal doses of bombesin and PMA combined was greater than that of either agent alone, suggesting that bombesin does not fully activate the PKC pathway. Accordingly, bombesin-induced PKCα translocation from cytosol to membranes was partial, while that observed with PMA was essentially complete. Moreover, exposure to Ro-31-8220, a PKC inhibitor, had signficantly greater effects on the response to PMA than on that to bombesin. Our findings point out different roles that PKCα may play in diversely activated cells: while, in the case of PMA, stimulation of this kinase may be necessary and sufficient to induce proliferation, it appears to be necessary only for a full response to bombesin, and redundant among the mechanisms triggered by PDGF. Fil:Florin-Christensen, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Florin-Christensen, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Meinardi, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02646021_v315_n2_p513_FlorinChristensen
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	2 [1 (3 amidinothiopropyl) 1h indol 3 yl] 3 (1 methyl 1h indol 3 yl)maleimide bombesin DNA phorbol 13 acetate 12 myristate platelet derived growth factor protein kinase C protein kinase C inhibitor animal cell article cell proliferation cell strain 3T3 controlled study DNA synthesis enzyme activation enzyme subunit mitogenesis mouse nonhuman priority journal protein transport Animalia
spellingShingle	2 [1 (3 amidinothiopropyl) 1h indol 3 yl] 3 (1 methyl 1h indol 3 yl)maleimide bombesin DNA phorbol 13 acetate 12 myristate platelet derived growth factor protein kinase C protein kinase C inhibitor animal cell article cell proliferation cell strain 3T3 controlled study DNA synthesis enzyme activation enzyme subunit mitogenesis mouse nonhuman priority journal protein transport Animalia Florin-Christensen, J. Florin-Christensen, M. Meinardi, E. Calle, R. Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells
topic_facet	2 [1 (3 amidinothiopropyl) 1h indol 3 yl] 3 (1 methyl 1h indol 3 yl)maleimide bombesin DNA phorbol 13 acetate 12 myristate platelet derived growth factor protein kinase C protein kinase C inhibitor animal cell article cell proliferation cell strain 3T3 controlled study DNA synthesis enzyme activation enzyme subunit mitogenesis mouse nonhuman priority journal protein transport Animalia
description	We examined the role of protein kinase Cα (PKCα) in the stimulation of DNA synthesis of Swiss 3T3 cells induced by bombesin, platelet-derived growth factor (PDGF) and phorbol 12-myristate 13-acetate (PMA). We found that cells in which this kinase had been down-regulated showed a partially abrogated mitogenic response to bombesin. The response to PDGF was unaltered; however, the response to PMA was completely suppressed. The mitogenic effect of maximal doses of bombesin and PMA combined was greater than that of either agent alone, suggesting that bombesin does not fully activate the PKC pathway. Accordingly, bombesin-induced PKCα translocation from cytosol to membranes was partial, while that observed with PMA was essentially complete. Moreover, exposure to Ro-31-8220, a PKC inhibitor, had signficantly greater effects on the response to PMA than on that to bombesin. Our findings point out different roles that PKCα may play in diversely activated cells: while, in the case of PMA, stimulation of this kinase may be necessary and sufficient to induce proliferation, it appears to be necessary only for a full response to bombesin, and redundant among the mechanisms triggered by PDGF.
format	JOUR
author	Florin-Christensen, J. Florin-Christensen, M. Meinardi, E. Calle, R.
author_facet	Florin-Christensen, J. Florin-Christensen, M. Meinardi, E. Calle, R.
author_sort	Florin-Christensen, J.
title	Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells
title_short	Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells
title_full	Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells
title_fullStr	Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells
title_full_unstemmed	Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells
title_sort	diversity of roles of protein kinase cα in the proliferation of swiss 3t3 cells
url	http://hdl.handle.net/20.500.12110/paper_02646021_v315_n2_p513_FlorinChristensen
work_keys_str_mv	AT florinchristensenj diversityofrolesofproteinkinasecaintheproliferationofswiss3t3cells AT florinchristensenm diversityofrolesofproteinkinasecaintheproliferationofswiss3t3cells AT meinardie diversityofrolesofproteinkinasecaintheproliferationofswiss3t3cells AT caller diversityofrolesofproteinkinasecaintheproliferationofswiss3t3cells
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Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells

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