Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes

The existence of a GTP-binding protein of the N(s) type in Trypanosoma cruzi was explored. Epimastigote membranes were labelled by cholera toxin in the presence of [adenine-14C]NAD+. After SDS/polyacrylamide-gel electrophoresis of extracted membrane proteins, a single labelled polypeptide band of ap...

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Autores principales: Eisenschlos, C.D., Paladini, A.A., Molina y Vedia, L.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02646021_v237_n3_p913_Eisenschlos
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Sumario:The existence of a GTP-binding protein of the N(s) type in Trypanosoma cruzi was explored. Epimastigote membranes were labelled by cholera toxin in the presence of [adenine-14C]NAD+. After SDS/polyacrylamide-gel electrophoresis of extracted membrane proteins, a single labelled polypeptide band of apparent M(r) approx. 45000 was detected. Epimastigote cells were treated with N-ethylmaleimide and electrofused to lymphoma S49 cells lacking the N(s) protein. Evidence indicates that in such electrofusion-generated cell hybrids a heterologous adenylate cyclase system was reconstituted with the N(s) protein provided by T. cruzi epimastigotes.