Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes
The existence of a GTP-binding protein of the N(s) type in Trypanosoma cruzi was explored. Epimastigote membranes were labelled by cholera toxin in the presence of [adenine-14C]NAD+. After SDS/polyacrylamide-gel electrophoresis of extracted membrane proteins, a single labelled polypeptide band of ap...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_02646021_v237_n3_p913_Eisenschlos |
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todo:paper_02646021_v237_n3_p913_Eisenschlos2023-10-03T15:12:54Z Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes Eisenschlos, C.D. Paladini, A.A. Molina y Vedia, L. cholera toxin polypeptide adenylate cyclase cholera toxin GTP Binding Proteins guanine nucleotide binding protein animal cell electrofusion epimastigote membrane nonhuman parasite priority journal protozoon Trypanosoma cruzi article cell fusion cell membrane drug effect electricity hybrid cell lymphoma metabolism Trypanosoma cruzi Animalia Protozoa Trypanosoma Trypanosoma cruzi Adenylate Cyclase Cell Fusion Cell Membrane Cholera Toxin Electricity GTP-Binding Proteins Hybrid Cells Lymphoma Support, Non-U.S. Gov't Trypanosoma cruzi The existence of a GTP-binding protein of the N(s) type in Trypanosoma cruzi was explored. Epimastigote membranes were labelled by cholera toxin in the presence of [adenine-14C]NAD+. After SDS/polyacrylamide-gel electrophoresis of extracted membrane proteins, a single labelled polypeptide band of apparent M(r) approx. 45000 was detected. Epimastigote cells were treated with N-ethylmaleimide and electrofused to lymphoma S49 cells lacking the N(s) protein. Evidence indicates that in such electrofusion-generated cell hybrids a heterologous adenylate cyclase system was reconstituted with the N(s) protein provided by T. cruzi epimastigotes. Fil:Molina y Vedia, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02646021_v237_n3_p913_Eisenschlos |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cholera toxin polypeptide adenylate cyclase cholera toxin GTP Binding Proteins guanine nucleotide binding protein animal cell electrofusion epimastigote membrane nonhuman parasite priority journal protozoon Trypanosoma cruzi article cell fusion cell membrane drug effect electricity hybrid cell lymphoma metabolism Trypanosoma cruzi Animalia Protozoa Trypanosoma Trypanosoma cruzi Adenylate Cyclase Cell Fusion Cell Membrane Cholera Toxin Electricity GTP-Binding Proteins Hybrid Cells Lymphoma Support, Non-U.S. Gov't Trypanosoma cruzi |
| spellingShingle |
cholera toxin polypeptide adenylate cyclase cholera toxin GTP Binding Proteins guanine nucleotide binding protein animal cell electrofusion epimastigote membrane nonhuman parasite priority journal protozoon Trypanosoma cruzi article cell fusion cell membrane drug effect electricity hybrid cell lymphoma metabolism Trypanosoma cruzi Animalia Protozoa Trypanosoma Trypanosoma cruzi Adenylate Cyclase Cell Fusion Cell Membrane Cholera Toxin Electricity GTP-Binding Proteins Hybrid Cells Lymphoma Support, Non-U.S. Gov't Trypanosoma cruzi Eisenschlos, C.D. Paladini, A.A. Molina y Vedia, L. Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes |
| topic_facet |
cholera toxin polypeptide adenylate cyclase cholera toxin GTP Binding Proteins guanine nucleotide binding protein animal cell electrofusion epimastigote membrane nonhuman parasite priority journal protozoon Trypanosoma cruzi article cell fusion cell membrane drug effect electricity hybrid cell lymphoma metabolism Trypanosoma cruzi Animalia Protozoa Trypanosoma Trypanosoma cruzi Adenylate Cyclase Cell Fusion Cell Membrane Cholera Toxin Electricity GTP-Binding Proteins Hybrid Cells Lymphoma Support, Non-U.S. Gov't Trypanosoma cruzi |
| description |
The existence of a GTP-binding protein of the N(s) type in Trypanosoma cruzi was explored. Epimastigote membranes were labelled by cholera toxin in the presence of [adenine-14C]NAD+. After SDS/polyacrylamide-gel electrophoresis of extracted membrane proteins, a single labelled polypeptide band of apparent M(r) approx. 45000 was detected. Epimastigote cells were treated with N-ethylmaleimide and electrofused to lymphoma S49 cells lacking the N(s) protein. Evidence indicates that in such electrofusion-generated cell hybrids a heterologous adenylate cyclase system was reconstituted with the N(s) protein provided by T. cruzi epimastigotes. |
| format |
JOUR |
| author |
Eisenschlos, C.D. Paladini, A.A. Molina y Vedia, L. |
| author_facet |
Eisenschlos, C.D. Paladini, A.A. Molina y Vedia, L. |
| author_sort |
Eisenschlos, C.D. |
| title |
Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes |
| title_short |
Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes |
| title_full |
Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes |
| title_fullStr |
Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes |
| title_full_unstemmed |
Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes |
| title_sort |
evidence for the existence of an n(s)-type regulatory protein in trypanosoma cruzi membranes |
| url |
http://hdl.handle.net/20.500.12110/paper_02646021_v237_n3_p913_Eisenschlos |
| work_keys_str_mv |
AT eisenschloscd evidencefortheexistenceofannstyperegulatoryproteinintrypanosomacruzimembranes AT paladiniaa evidencefortheexistenceofannstyperegulatoryproteinintrypanosomacruzimembranes AT molinayvedial evidencefortheexistenceofannstyperegulatoryproteinintrypanosomacruzimembranes |
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1807320061296246784 |