Vaccination with dendritic cells charged with apoptotic/necrotic B16 melanoma induces the formation of subcutaneous lymphoid tissue
Antigen presentation by dendritic cells (DC) is of key importance for the initiation of the primary immune response. Mice vaccinated with DC charged with apoptotic/necrotic B16 cells (DC-Apo/Nec) are protected against B16 challenge. The aim of this study was to assess vaccine cell migration in our s...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0264410X_v28_n51_p8162_Keon |
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todo:paper_0264410X_v28_n51_p8162_Keon2023-10-03T15:12:50Z Vaccination with dendritic cells charged with apoptotic/necrotic B16 melanoma induces the formation of subcutaneous lymphoid tissue Keon, S.M. Gazzaniga, S. Mallerman, J. Bravo, A.I. Mordoh, J. Wainstok, R. Apoptotic cell Dendritic cell Lymphoid tissue Melanoma Vaccination addressin dendritic cell vaccine animal experiment animal model animal tissue apoptosis article CD4+ T lymphocyte CD8+ T lymphocyte cell migration cell population controlled study immune response immunity lymphoid tissue macrophage male melanoma mouse nonhuman polymorphonuclear cell priority journal protein expression subcutaneous lymphoid tissue vaccination venule Animals Antigens, Neoplasm Cancer Vaccines Dendritic Cells Histocytochemistry Injections, Subcutaneous Lymphoid Tissue Male Melanoma, Experimental Mice Mice, Inbred C57BL Subcutaneous Tissue Survival Analysis Vaccination Antigen presentation by dendritic cells (DC) is of key importance for the initiation of the primary immune response. Mice vaccinated with DC charged with apoptotic/necrotic B16 cells (DC-Apo/Nec) are protected against B16 challenge. The aim of this study was to assess vaccine cell migration in our system and to find out if there is an immunological response taking place at the vaccination site. The formation of a pseudocapsule, peripheral node addresin expression in small venules, and the recruitment of a wide variety of cellular populations, including macrophages, polymorphonuclear lymphocytes, and CD8+ and CD4+ T lymphocytes found in association with DC, evidenced the formation of tertiary lymphoid tissue in the vaccination site in our experimental system. © 2010 Elsevier Ltd. Fil:Gazzaniga, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mallerman, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0264410X_v28_n51_p8162_Keon |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptotic cell Dendritic cell Lymphoid tissue Melanoma Vaccination addressin dendritic cell vaccine animal experiment animal model animal tissue apoptosis article CD4+ T lymphocyte CD8+ T lymphocyte cell migration cell population controlled study immune response immunity lymphoid tissue macrophage male melanoma mouse nonhuman polymorphonuclear cell priority journal protein expression subcutaneous lymphoid tissue vaccination venule Animals Antigens, Neoplasm Cancer Vaccines Dendritic Cells Histocytochemistry Injections, Subcutaneous Lymphoid Tissue Male Melanoma, Experimental Mice Mice, Inbred C57BL Subcutaneous Tissue Survival Analysis Vaccination |
| spellingShingle |
Apoptotic cell Dendritic cell Lymphoid tissue Melanoma Vaccination addressin dendritic cell vaccine animal experiment animal model animal tissue apoptosis article CD4+ T lymphocyte CD8+ T lymphocyte cell migration cell population controlled study immune response immunity lymphoid tissue macrophage male melanoma mouse nonhuman polymorphonuclear cell priority journal protein expression subcutaneous lymphoid tissue vaccination venule Animals Antigens, Neoplasm Cancer Vaccines Dendritic Cells Histocytochemistry Injections, Subcutaneous Lymphoid Tissue Male Melanoma, Experimental Mice Mice, Inbred C57BL Subcutaneous Tissue Survival Analysis Vaccination Keon, S.M. Gazzaniga, S. Mallerman, J. Bravo, A.I. Mordoh, J. Wainstok, R. Vaccination with dendritic cells charged with apoptotic/necrotic B16 melanoma induces the formation of subcutaneous lymphoid tissue |
| topic_facet |
Apoptotic cell Dendritic cell Lymphoid tissue Melanoma Vaccination addressin dendritic cell vaccine animal experiment animal model animal tissue apoptosis article CD4+ T lymphocyte CD8+ T lymphocyte cell migration cell population controlled study immune response immunity lymphoid tissue macrophage male melanoma mouse nonhuman polymorphonuclear cell priority journal protein expression subcutaneous lymphoid tissue vaccination venule Animals Antigens, Neoplasm Cancer Vaccines Dendritic Cells Histocytochemistry Injections, Subcutaneous Lymphoid Tissue Male Melanoma, Experimental Mice Mice, Inbred C57BL Subcutaneous Tissue Survival Analysis Vaccination |
| description |
Antigen presentation by dendritic cells (DC) is of key importance for the initiation of the primary immune response. Mice vaccinated with DC charged with apoptotic/necrotic B16 cells (DC-Apo/Nec) are protected against B16 challenge. The aim of this study was to assess vaccine cell migration in our system and to find out if there is an immunological response taking place at the vaccination site. The formation of a pseudocapsule, peripheral node addresin expression in small venules, and the recruitment of a wide variety of cellular populations, including macrophages, polymorphonuclear lymphocytes, and CD8+ and CD4+ T lymphocytes found in association with DC, evidenced the formation of tertiary lymphoid tissue in the vaccination site in our experimental system. © 2010 Elsevier Ltd. |
| format |
JOUR |
| author |
Keon, S.M. Gazzaniga, S. Mallerman, J. Bravo, A.I. Mordoh, J. Wainstok, R. |
| author_facet |
Keon, S.M. Gazzaniga, S. Mallerman, J. Bravo, A.I. Mordoh, J. Wainstok, R. |
| author_sort |
Keon, S.M. |
| title |
Vaccination with dendritic cells charged with apoptotic/necrotic B16 melanoma induces the formation of subcutaneous lymphoid tissue |
| title_short |
Vaccination with dendritic cells charged with apoptotic/necrotic B16 melanoma induces the formation of subcutaneous lymphoid tissue |
| title_full |
Vaccination with dendritic cells charged with apoptotic/necrotic B16 melanoma induces the formation of subcutaneous lymphoid tissue |
| title_fullStr |
Vaccination with dendritic cells charged with apoptotic/necrotic B16 melanoma induces the formation of subcutaneous lymphoid tissue |
| title_full_unstemmed |
Vaccination with dendritic cells charged with apoptotic/necrotic B16 melanoma induces the formation of subcutaneous lymphoid tissue |
| title_sort |
vaccination with dendritic cells charged with apoptotic/necrotic b16 melanoma induces the formation of subcutaneous lymphoid tissue |
| url |
http://hdl.handle.net/20.500.12110/paper_0264410X_v28_n51_p8162_Keon |
| work_keys_str_mv |
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| _version_ |
1807323709729406976 |