Involvement of membrane tubulin in erythrocyte deformability and blood pressure

Objective: To test the hypothesis that erythrocyte deformability is influenced by changes in the content of membrane tubulin (Mem-tub). Methods and Results: Human erythrocytes contain tubulin distributed in three pools (membrane, sedimentable, soluble). Erythrocytes from hypertensive humans have a h...

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Detalles Bibliográficos
Autores principales: Amaiden, M.R., Monesterolo, N.E., Santander, V.S., Campetelli, A.N., Arce, C.A., Pie, J., Hope, S.I., Vatta, M.S., Casale, C.H.
Formato: JOUR
Lenguaje:English
Materias:
blood pressure
deformability
human erythrocytes
hypertensive patients
K +-ATPase
membrane
Na +
tubulin
adenosine triphosphatase (potassium sodium)
adenosine triphosphatase (potassium)
nocodazole
paclitaxel
animal cell
animal experiment
animal model
article
cell free system
concentration (parameters)
controlled study
correlational study
erythrocyte deformability
erythrocyte membrane
female
human
human cell
hypertension
male
nonhuman
priority journal
protein localization
protein modification
rat
spontaneously hypertensive rat
Adult
Animals
Blood Pressure
Cell Membrane
Erythrocyte Deformability
Humans
Hypertension
Male
Membrane Proteins
Microscopy, Fluorescence
Nocodazole
Paclitaxel
Rats
Rats, Inbred SHR
Rats, Wistar
Tubulin
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02636352_v30_n7_p1414_Amaiden
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Objective: To test the hypothesis that erythrocyte deformability is influenced by changes in the content of membrane tubulin (Mem-tub). Methods and Results: Human erythrocytes contain tubulin distributed in three pools (membrane, sedimentable, soluble). Erythrocytes from hypertensive humans have a higher proportion of Mem-tub. Increased Mem-tub content in hypertensive patients was correlated with decreased erythrocyte deformability. Treatment of erythrocytes from normotensive individuals with taxol increased Mem-tub content and reduced deformability, whereas treatment of hypertensive patients erythrocytes with nocodazole had the opposite effect. In-vivo experiments with rats were performed to examine the possible relationship between Mem-tub content, erythrocyte deformability, and blood pressure. Spontaneously hypertensive rats (SHRs) showed lower erythrocyte deformability than normotensive Wistar rats. During the development of hypertension in SHR, tubulin in erythrocytes is translocated to the membrane, and this process is correlated with decreased deformability. In-vivo treatment (intraperitoneal injection) of SHR with nocodazole decreased Mem-tub content, increased erythrocyte deformability, and decreased blood pressure, whereas treatment of Wistar rats with taxol had the opposite effects. Conclusion: These findings indicate that increased Mem-tub content contributes to reduced erythrocyte deformability in hypertensive animals. © 2012 Wolters Kluwer Health | Lippincott Williams & Wilkins.

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