Involvement of membrane tubulin in erythrocyte deformability and blood pressure
Objective: To test the hypothesis that erythrocyte deformability is influenced by changes in the content of membrane tubulin (Mem-tub). Methods and Results: Human erythrocytes contain tubulin distributed in three pools (membrane, sedimentable, soluble). Erythrocytes from hypertensive humans have a h...
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| Lenguaje: | English |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_02636352_v30_n7_p1414_Amaiden |
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todo:paper_02636352_v30_n7_p1414_Amaiden2023-10-03T15:12:41Z Involvement of membrane tubulin in erythrocyte deformability and blood pressure Amaiden, M.R. Monesterolo, N.E. Santander, V.S. Campetelli, A.N. Arce, C.A. Pie, J. Hope, S.I. Vatta, M.S. Casale, C.H. blood pressure deformability human erythrocytes hypertensive patients K +-ATPase membrane Na + tubulin adenosine triphosphatase (potassium sodium) adenosine triphosphatase (potassium) nocodazole paclitaxel tubulin animal cell animal experiment animal model article blood pressure cell free system concentration (parameters) controlled study correlational study erythrocyte deformability erythrocyte membrane female human human cell hypertension male nonhuman priority journal protein localization protein modification rat spontaneously hypertensive rat Adult Animals Blood Pressure Cell Membrane Erythrocyte Deformability Humans Hypertension Male Membrane Proteins Microscopy, Fluorescence Nocodazole Paclitaxel Rats Rats, Inbred SHR Rats, Wistar Tubulin Objective: To test the hypothesis that erythrocyte deformability is influenced by changes in the content of membrane tubulin (Mem-tub). Methods and Results: Human erythrocytes contain tubulin distributed in three pools (membrane, sedimentable, soluble). Erythrocytes from hypertensive humans have a higher proportion of Mem-tub. Increased Mem-tub content in hypertensive patients was correlated with decreased erythrocyte deformability. Treatment of erythrocytes from normotensive individuals with taxol increased Mem-tub content and reduced deformability, whereas treatment of hypertensive patients erythrocytes with nocodazole had the opposite effect. In-vivo experiments with rats were performed to examine the possible relationship between Mem-tub content, erythrocyte deformability, and blood pressure. Spontaneously hypertensive rats (SHRs) showed lower erythrocyte deformability than normotensive Wistar rats. During the development of hypertension in SHR, tubulin in erythrocytes is translocated to the membrane, and this process is correlated with decreased deformability. In-vivo treatment (intraperitoneal injection) of SHR with nocodazole decreased Mem-tub content, increased erythrocyte deformability, and decreased blood pressure, whereas treatment of Wistar rats with taxol had the opposite effects. Conclusion: These findings indicate that increased Mem-tub content contributes to reduced erythrocyte deformability in hypertensive animals. © 2012 Wolters Kluwer Health | Lippincott Williams & Wilkins. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02636352_v30_n7_p1414_Amaiden |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
English |
| orig_language_str_mv |
English |
| topic |
blood pressure deformability human erythrocytes hypertensive patients K +-ATPase membrane Na + tubulin adenosine triphosphatase (potassium sodium) adenosine triphosphatase (potassium) nocodazole paclitaxel tubulin animal cell animal experiment animal model article blood pressure cell free system concentration (parameters) controlled study correlational study erythrocyte deformability erythrocyte membrane female human human cell hypertension male nonhuman priority journal protein localization protein modification rat spontaneously hypertensive rat Adult Animals Blood Pressure Cell Membrane Erythrocyte Deformability Humans Hypertension Male Membrane Proteins Microscopy, Fluorescence Nocodazole Paclitaxel Rats Rats, Inbred SHR Rats, Wistar Tubulin |
| spellingShingle |
blood pressure deformability human erythrocytes hypertensive patients K +-ATPase membrane Na + tubulin adenosine triphosphatase (potassium sodium) adenosine triphosphatase (potassium) nocodazole paclitaxel tubulin animal cell animal experiment animal model article blood pressure cell free system concentration (parameters) controlled study correlational study erythrocyte deformability erythrocyte membrane female human human cell hypertension male nonhuman priority journal protein localization protein modification rat spontaneously hypertensive rat Adult Animals Blood Pressure Cell Membrane Erythrocyte Deformability Humans Hypertension Male Membrane Proteins Microscopy, Fluorescence Nocodazole Paclitaxel Rats Rats, Inbred SHR Rats, Wistar Tubulin Amaiden, M.R. Monesterolo, N.E. Santander, V.S. Campetelli, A.N. Arce, C.A. Pie, J. Hope, S.I. Vatta, M.S. Casale, C.H. Involvement of membrane tubulin in erythrocyte deformability and blood pressure |
| topic_facet |
blood pressure deformability human erythrocytes hypertensive patients K +-ATPase membrane Na + tubulin adenosine triphosphatase (potassium sodium) adenosine triphosphatase (potassium) nocodazole paclitaxel tubulin animal cell animal experiment animal model article blood pressure cell free system concentration (parameters) controlled study correlational study erythrocyte deformability erythrocyte membrane female human human cell hypertension male nonhuman priority journal protein localization protein modification rat spontaneously hypertensive rat Adult Animals Blood Pressure Cell Membrane Erythrocyte Deformability Humans Hypertension Male Membrane Proteins Microscopy, Fluorescence Nocodazole Paclitaxel Rats Rats, Inbred SHR Rats, Wistar Tubulin |
| description |
Objective: To test the hypothesis that erythrocyte deformability is influenced by changes in the content of membrane tubulin (Mem-tub). Methods and Results: Human erythrocytes contain tubulin distributed in three pools (membrane, sedimentable, soluble). Erythrocytes from hypertensive humans have a higher proportion of Mem-tub. Increased Mem-tub content in hypertensive patients was correlated with decreased erythrocyte deformability. Treatment of erythrocytes from normotensive individuals with taxol increased Mem-tub content and reduced deformability, whereas treatment of hypertensive patients erythrocytes with nocodazole had the opposite effect. In-vivo experiments with rats were performed to examine the possible relationship between Mem-tub content, erythrocyte deformability, and blood pressure. Spontaneously hypertensive rats (SHRs) showed lower erythrocyte deformability than normotensive Wistar rats. During the development of hypertension in SHR, tubulin in erythrocytes is translocated to the membrane, and this process is correlated with decreased deformability. In-vivo treatment (intraperitoneal injection) of SHR with nocodazole decreased Mem-tub content, increased erythrocyte deformability, and decreased blood pressure, whereas treatment of Wistar rats with taxol had the opposite effects. Conclusion: These findings indicate that increased Mem-tub content contributes to reduced erythrocyte deformability in hypertensive animals. © 2012 Wolters Kluwer Health | Lippincott Williams & Wilkins. |
| format |
JOUR |
| author |
Amaiden, M.R. Monesterolo, N.E. Santander, V.S. Campetelli, A.N. Arce, C.A. Pie, J. Hope, S.I. Vatta, M.S. Casale, C.H. |
| author_facet |
Amaiden, M.R. Monesterolo, N.E. Santander, V.S. Campetelli, A.N. Arce, C.A. Pie, J. Hope, S.I. Vatta, M.S. Casale, C.H. |
| author_sort |
Amaiden, M.R. |
| title |
Involvement of membrane tubulin in erythrocyte deformability and blood pressure |
| title_short |
Involvement of membrane tubulin in erythrocyte deformability and blood pressure |
| title_full |
Involvement of membrane tubulin in erythrocyte deformability and blood pressure |
| title_fullStr |
Involvement of membrane tubulin in erythrocyte deformability and blood pressure |
| title_full_unstemmed |
Involvement of membrane tubulin in erythrocyte deformability and blood pressure |
| title_sort |
involvement of membrane tubulin in erythrocyte deformability and blood pressure |
| url |
http://hdl.handle.net/20.500.12110/paper_02636352_v30_n7_p1414_Amaiden |
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