Expression of gelatinise/type IV collagenase in tumor necrosis correlates with cell detachment and tumor invasion
We have previously observed that acellular extracts from necrotic areas (NE) of the non-metastatic murine mammary adenocarcinoma M3, enhance in vitro cell detachment and spontaneous lung metastases. In the present study, using different proteinase inhibitors along with NE, only the calcium chelator...
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todo:paper_02620898_v10_n3_p211_DanielBonfil2023-10-03T15:12:33Z Expression of gelatinise/type IV collagenase in tumor necrosis correlates with cell detachment and tumor invasion Daniel Bonfil, R. Medina, P.A. Gómez, D.E. Farias, E. Lazarowski, A. Gritti, M.F.L. Meiss, R.P. Bustuoabad, O.D. gelatinase metalloproteinases tumor invasion tumor necrosis type IV collagenase antibody collagenase edetic acid gelatinase proteinase inhibitor animal model article breast adenocarcinoma cancer invasion cell division cell line controlled study enzyme activity female metastasis mouse nonhuman tumor necrosis Animal Cell Adhesion Collagen Extracellular Matrix Female Gelatinases Mice Mice, Inbred BALB C Microbial Collagenase Necrosis Neoplasm Invasiveness Neoplasms, Experimental Pepsin A Protease Inhibitors Support, Non-U.S. Gov't We have previously observed that acellular extracts from necrotic areas (NE) of the non-metastatic murine mammary adenocarcinoma M3, enhance in vitro cell detachment and spontaneous lung metastases. In the present study, using different proteinase inhibitors along with NE, only the calcium chelator EDTA could significantly abrogate the enhanced cell detachment from M3 produced by NE. The typical cleavage products of type IV collagenase were detected inside the tumor necrotic area, mainly in association with necrobiotic cells, as evaluated by Western blot analysis and immunohistochemical assays. Zymography revealed the presence of 72- and 92-kDa gelatinise/type IV collagenase in NE. Moreover, NE increased the in vitro invasive ability of cultured M3 cells. The use of specific antibodies against both 72- and 92-kDa type IV collagenases in the invasion assay showed that only the latter was able to revert the enhanced invasiveness to the baseline. It can be concluded that tumor necrosis is an important source of gelatinise/type IV collagenase, mainly in its 92 kDa form, and plays a major role in tumor invasion. © 1992 Rapid Communications of Oxford Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02620898_v10_n3_p211_DanielBonfil |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
gelatinase metalloproteinases tumor invasion tumor necrosis type IV collagenase antibody collagenase edetic acid gelatinase proteinase inhibitor animal model article breast adenocarcinoma cancer invasion cell division cell line controlled study enzyme activity female metastasis mouse nonhuman tumor necrosis Animal Cell Adhesion Collagen Extracellular Matrix Female Gelatinases Mice Mice, Inbred BALB C Microbial Collagenase Necrosis Neoplasm Invasiveness Neoplasms, Experimental Pepsin A Protease Inhibitors Support, Non-U.S. Gov't |
| spellingShingle |
gelatinase metalloproteinases tumor invasion tumor necrosis type IV collagenase antibody collagenase edetic acid gelatinase proteinase inhibitor animal model article breast adenocarcinoma cancer invasion cell division cell line controlled study enzyme activity female metastasis mouse nonhuman tumor necrosis Animal Cell Adhesion Collagen Extracellular Matrix Female Gelatinases Mice Mice, Inbred BALB C Microbial Collagenase Necrosis Neoplasm Invasiveness Neoplasms, Experimental Pepsin A Protease Inhibitors Support, Non-U.S. Gov't Daniel Bonfil, R. Medina, P.A. Gómez, D.E. Farias, E. Lazarowski, A. Gritti, M.F.L. Meiss, R.P. Bustuoabad, O.D. Expression of gelatinise/type IV collagenase in tumor necrosis correlates with cell detachment and tumor invasion |
| topic_facet |
gelatinase metalloproteinases tumor invasion tumor necrosis type IV collagenase antibody collagenase edetic acid gelatinase proteinase inhibitor animal model article breast adenocarcinoma cancer invasion cell division cell line controlled study enzyme activity female metastasis mouse nonhuman tumor necrosis Animal Cell Adhesion Collagen Extracellular Matrix Female Gelatinases Mice Mice, Inbred BALB C Microbial Collagenase Necrosis Neoplasm Invasiveness Neoplasms, Experimental Pepsin A Protease Inhibitors Support, Non-U.S. Gov't |
| description |
We have previously observed that acellular extracts from necrotic areas (NE) of the non-metastatic murine mammary adenocarcinoma M3, enhance in vitro cell detachment and spontaneous lung metastases. In the present study, using different proteinase inhibitors along with NE, only the calcium chelator EDTA could significantly abrogate the enhanced cell detachment from M3 produced by NE. The typical cleavage products of type IV collagenase were detected inside the tumor necrotic area, mainly in association with necrobiotic cells, as evaluated by Western blot analysis and immunohistochemical assays. Zymography revealed the presence of 72- and 92-kDa gelatinise/type IV collagenase in NE. Moreover, NE increased the in vitro invasive ability of cultured M3 cells. The use of specific antibodies against both 72- and 92-kDa type IV collagenases in the invasion assay showed that only the latter was able to revert the enhanced invasiveness to the baseline. It can be concluded that tumor necrosis is an important source of gelatinise/type IV collagenase, mainly in its 92 kDa form, and plays a major role in tumor invasion. © 1992 Rapid Communications of Oxford Ltd. |
| format |
JOUR |
| author |
Daniel Bonfil, R. Medina, P.A. Gómez, D.E. Farias, E. Lazarowski, A. Gritti, M.F.L. Meiss, R.P. Bustuoabad, O.D. |
| author_facet |
Daniel Bonfil, R. Medina, P.A. Gómez, D.E. Farias, E. Lazarowski, A. Gritti, M.F.L. Meiss, R.P. Bustuoabad, O.D. |
| author_sort |
Daniel Bonfil, R. |
| title |
Expression of gelatinise/type IV collagenase in tumor necrosis correlates with cell detachment and tumor invasion |
| title_short |
Expression of gelatinise/type IV collagenase in tumor necrosis correlates with cell detachment and tumor invasion |
| title_full |
Expression of gelatinise/type IV collagenase in tumor necrosis correlates with cell detachment and tumor invasion |
| title_fullStr |
Expression of gelatinise/type IV collagenase in tumor necrosis correlates with cell detachment and tumor invasion |
| title_full_unstemmed |
Expression of gelatinise/type IV collagenase in tumor necrosis correlates with cell detachment and tumor invasion |
| title_sort |
expression of gelatinise/type iv collagenase in tumor necrosis correlates with cell detachment and tumor invasion |
| url |
http://hdl.handle.net/20.500.12110/paper_02620898_v10_n3_p211_DanielBonfil |
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