β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation
The aim of this work was to build, to isolate and to characterize, core-shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core-shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and fi...
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| Autores principales: | , , , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_02608774_v131_n_p65_Carpineti |
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| Sumario: | The aim of this work was to build, to isolate and to characterize, core-shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core-shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and finally, decreasing the pH up to 4, promoting the adsorption of CMC on the protein core due their opposite electric charge. The core-shell microparticles were characterized by static laser light scattering (SLS), optical microscopy and atomic force microscopy (AFM). At pH 4, (β-lg)n showed a diameter ∼200 nm, but after adding the polysaccharide had a diameter ∼1 μm. The microscopy corroborated the data obtained by SLS measurements. Core-shell microparticles could be isolated by lyophilization and potentially applied as a fat replacement and/or a delivery systems for encapsulated substances in food formulations. © 2014 Elsevier Ltd. All rights reserved. |
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