β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation

The aim of this work was to build, to isolate and to characterize, core-shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core-shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and fi...

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Autores principales:	Carpineti, L., Martinez, M.J., Pilosof, A.M.R., Pérez, O.E.
Formato:	JOUR
Materias:	β-Lactoglobulin Carboximethylcellulose Core-shell microparticles Electrostatic deposition Lyophilization
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_02608774_v131_n_p65_Carpineti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_02608774_v131_n_p65_Carpineti
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spelling	todo:paper_02608774_v131_n_p65_Carpineti2023-10-03T15:11:57Z β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation Carpineti, L. Martinez, M.J. Pilosof, A.M.R. Pérez, O.E. β-Lactoglobulin Carboximethylcellulose Core-shell microparticles Electrostatic deposition Lyophilization The aim of this work was to build, to isolate and to characterize, core-shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core-shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and finally, decreasing the pH up to 4, promoting the adsorption of CMC on the protein core due their opposite electric charge. The core-shell microparticles were characterized by static laser light scattering (SLS), optical microscopy and atomic force microscopy (AFM). At pH 4, (β-lg)n showed a diameter ∼200 nm, but after adding the polysaccharide had a diameter ∼1 μm. The microscopy corroborated the data obtained by SLS measurements. Core-shell microparticles could be isolated by lyophilization and potentially applied as a fat replacement and/or a delivery systems for encapsulated substances in food formulations. © 2014 Elsevier Ltd. All rights reserved. Fil:Martinez, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérez, O.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02608774_v131_n_p65_Carpineti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	β-Lactoglobulin Carboximethylcellulose Core-shell microparticles Electrostatic deposition Lyophilization
spellingShingle	β-Lactoglobulin Carboximethylcellulose Core-shell microparticles Electrostatic deposition Lyophilization Carpineti, L. Martinez, M.J. Pilosof, A.M.R. Pérez, O.E. β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation
topic_facet	β-Lactoglobulin Carboximethylcellulose Core-shell microparticles Electrostatic deposition Lyophilization
description	The aim of this work was to build, to isolate and to characterize, core-shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core-shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and finally, decreasing the pH up to 4, promoting the adsorption of CMC on the protein core due their opposite electric charge. The core-shell microparticles were characterized by static laser light scattering (SLS), optical microscopy and atomic force microscopy (AFM). At pH 4, (β-lg)n showed a diameter ∼200 nm, but after adding the polysaccharide had a diameter ∼1 μm. The microscopy corroborated the data obtained by SLS measurements. Core-shell microparticles could be isolated by lyophilization and potentially applied as a fat replacement and/or a delivery systems for encapsulated substances in food formulations. © 2014 Elsevier Ltd. All rights reserved.
format	JOUR
author	Carpineti, L. Martinez, M.J. Pilosof, A.M.R. Pérez, O.E.
author_facet	Carpineti, L. Martinez, M.J. Pilosof, A.M.R. Pérez, O.E.
author_sort	Carpineti, L.
title	β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation
title_short	β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation
title_full	β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation
title_fullStr	β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation
title_full_unstemmed	β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation
title_sort	β-lactoglobulin-carboxymethylcellulose core-shell microparticles: construction, characterization and isolation
url	http://hdl.handle.net/20.500.12110/paper_02608774_v131_n_p65_Carpineti
work_keys_str_mv	AT carpinetil blactoglobulincarboxymethylcellulosecoreshellmicroparticlesconstructioncharacterizationandisolation AT martinezmj blactoglobulincarboxymethylcellulosecoreshellmicroparticlesconstructioncharacterizationandisolation AT pilosofamr blactoglobulincarboxymethylcellulosecoreshellmicroparticlesconstructioncharacterizationandisolation AT perezoe blactoglobulincarboxymethylcellulosecoreshellmicroparticlesconstructioncharacterizationandisolation
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β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation

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